Combination Therapy with Curcumin Alone Plus Piperine Ameliorates Ovalbumin-Induced Chronic Asthma in Mice
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Allergic asthma is an inflammatory condition accompanied by inflammation as well as oxidative stress. Supplementation of an anti-inflammatory agent having antioxidant properties may have therapeutic effects against this disease. Over the recent decades, the interest in combination therapy as new alternative medication has increased and it offers numerous benefits along with noticeable lack of toxicity as well as side effects. In this study, protective effects of curcumin alone and in combination with piperine were evaluated in mouse model of allergic asthma. Balb/c mice were sensitized on days 0, 7, and 14 and challenged from days 16–30 on alternate days with ovalbumin (OVA). Mice were pretreated with curcumin (Cur; 10 and 20 mg/kg) and piperine (Pip; 5 mg/kg) alone and in combination via the intraperitoneal route on days 16–30 and compared with intranasal curcumin (5 mg/kg) treatment. Blood, bronchoalveolar lavage fluid (BALF), and lungs were collected after mice were sacrificed on day 31st. Mice immunized with OVA have shown significant increase in airway inflammation and oxidative stress as determined by oxidative stress markers. A significant suppression was observed with all the treatments, but intranasal curcumin treatment group has shown maximum suppression. So, among all the treatment strategies utilized, intranasal curcumin administration was most appropriate in reducing inflammation and oxidative stress and possesses therapeutic potential against allergic asthma. Present study may prove the possibility of development of curcumin nasal drops towards treatment of allergic asthma.
KEY WORDSinflammation oxidative stress ovalbumin curcumin piperine asthma
The authors are thankful to Prof. Dash for providing spectrofluorometer facility to analyze reactive oxygen species (ROS) by using DCF-DA fluorescence at the Department of Biochemistry, Institute of Medical Sciences, Banaras Hindu University, Varanasi. We are also thankful to University Grants Commission (UGC), DST-Science and Engineering Research Board (DST-SERB), and Council of Scientific and Industrial Research (CSIR), New Delhi, India for financial assistance.
Compliance with Ethical Standards
The study design was approved by the Institutional Animal Ethical Committee, Banaras Hindu University, Varanasi.
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