, Volume 41, Issue 5, pp 1671–1680 | Cite as

Astragaloside IV Inhibits Cigarette Smoke-Induced Pulmonary Inflammation in Mice

  • Zhou Meiqian
  • Zhuo Leying
  • Cai ChangEmail author


The aim of this study was to investigate the effects of Astragaloside IV (AS) on cigarette smoke (CS)-induced chronic obstructive pulmonary disease (COPD). Our results showed that AS alleviated CS-induced pathological injury in lung tissue. AS also increased superoxide dismutase (SOD) and reduced the level of malondialdehyde (MDA) in serum and lung. AS also reduced cytokines including tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and interleukin-1β (IL-1β) in serum and lung. More, AS significantly reduced the protein expression of JAK3/STAT3/NF-κB pathway in CS-induced mice. In vitro, cigarette smoke extract (CSE) stimulation exposed to normal human bronchial epithelial (HBE) cells. Results further confirmed that AS significantly inhibited the protein levels of JAK3/STAT3/NF-κB pathway in CSE-induced HBE. Our result showed that AS might effectively ameliorate COPD via JAK3/STAT3/NF-κB pathway.


Astragaloside IV COPD inflammation 


Funding Information

This work was supported by National Natural Science Project (81470225).

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflict of interest.


  1. 1.
    Johnschuster, G., et al. 2016. Inflammaging increases susceptibility to cigarette smoke-induced COPD. Oncotarget 7 (21): 30068–30083.Google Scholar
  2. 2.
    Magrone, T., and E. Jirillo. 2016. Cigarette smoke-mediated perturbations of the immune response: a new therapeutic approach with natural compounds. Endocrine Metabolic & Immune Disorders Drug Targets 16 (3): 158–167.Google Scholar
  3. 3.
    Vij, N., Chandramani-Shivalingappa, P., Van Westphal, C., Hole, R., Bodas, M. 2016. Cigarette smoke induced autophagy-impairment accelerates lung aging, COPD-emphysema exacerbations and pathogenesis. American Journal of Physiology Cell Physiology 314 (1): C73–C87.Google Scholar
  4. 4.
    Shao, P., L.H. Zhao, Zhi-Chen, and J.P. Pan. 2006. Regulation on maturation and function of dendritic cells by Astragalus mongholicus polysaccharides. International Immunopharmacology 6 (7): 1161–1166.CrossRefPubMedGoogle Scholar
  5. 5.
    Meng, L., J.W. Tang, Y. Wang, J.R. Zhao, M.Y. Shang, M. Zhang, S.Y. Liu, L. Qu, S.Q. Cai, and X.M. Li. 2011. Astragaloside IV synergizes with ferulic acid to inhibit renal tubulointerstitial fibrosis in rats with obstructive nephropathy. British Journal of Pharmacology 162 (8): 1805–1818.CrossRefPubMedPubMedCentralGoogle Scholar
  6. 6.
    Chung-Min, Y., et al. 2016. Epigenetic silencing of theNR4A3tumor suppressor, by aberrant JAK/STAT signaling, predicts prognosis in gastric cancer. Scientific Reports 6: 31690.CrossRefGoogle Scholar
  7. 7.
    Ahmad, S.F., M.A. Ansari, K.M.A. Zoheir, S.A. Bakheet, H.M. Korashy, A. Nadeem, A.E. Ashour, and S.M. Attia. 2015. Regulation of TNF-α and NF-κB activation through the JAK/STAT signaling pathway downstream of histamine 4 receptor in a rat model of LPS-induced joint inflammation. Immunobiology 220 (7): 889–898.CrossRefPubMedGoogle Scholar
  8. 8.
    Jia, Y., J. Jing, Y. Bai, Z. Li, L. Liu, J. Luo, M. Liu, and H. Chen. 2011. Amelioration of experimental autoimmune encephalomyelitis by plumbagin through down-regulation of JAK-STAT and NF-κB signaling pathways. PLoS One 6 (6): e27006.CrossRefPubMedPubMedCentralGoogle Scholar
  9. 9.
    Primack, B.A., M.V. Carroll, P.M. Weiss, A.L. Shihadeh, A. Shensa, S.T. Farley, M.J. Fine, T. Eissenberg, and S. Nayak. 2016. Systematic review and meta-analysis of inhaled toxicants from waterpipe and cigarette smoking. Public Health Reports 131 (1): 76–85.CrossRefPubMedPubMedCentralGoogle Scholar
  10. 10.
    Wu, Y.P., C. Cao, Y.F. Wu, M. Li, T.W. Lai, C. Zhu, Y. Wang, S.M. Ying, Z.H. Chen, H.H. Shen, and W. Li. 2017. Activating transcription factor 3 represses cigarette smoke-induced IL6 and IL8 expression via suppressing NF-κB activation. Toxicology Letters 270: 17–24.CrossRefPubMedGoogle Scholar
  11. 11.
    XY, Z., et al. 2017. Plumbagin suppresses chronic periodontitis in rats via down-regulation of TNF-α, IL-1β and IL-6 expression. Acta Pharmacologica Sinica.Google Scholar
  12. 12.
    Li, Y., et al. 2017. Effects of ketamine on levels of inflammatory cytokines IL-6, IL-1β, and TNF-α in the hippocampus of mice following acute or chronic administration. Frontiers in Pharmacology 8: 139.PubMedPubMedCentralGoogle Scholar
  13. 13.
    Khan, J., N. Noboru, A. Young, and D. Thomas. 2017. Pro and anti-inflammatory cytokine levels (TNF-α, IL-1β, IL-6 and IL-10) in rat model of neuroma. Pathophysiology 24: 155–159.CrossRefPubMedGoogle Scholar
  14. 14.
    Athanasios, V., et al. 2013. Pulmonary oxidative stress, inflammation and cancer: respirable particulate matter, fibrous dusts and ozone as major causes of lung carcinogenesis through reactive oxygen species mechanisms. International Journal of Environmental Research and Public Health 10 (9): 3886–3907.CrossRefGoogle Scholar
  15. 15.
    Kang, K.A., Z.H. Wang, R. Zhang, M.J. Piao, K.C. Kim, S.S. Kang, Y.W. Kim, J. Lee, D. Park, and J.W. Hyun. 2010. Myricetin protects cells against oxidative stress-induced apoptosis via regulation of PI3K/Akt and MAPK signaling pathways. International Journal of Molecular Sciences 11 (11): 4348–4360.CrossRefPubMedPubMedCentralGoogle Scholar
  16. 16.
    Zhang, H., Z. Ji, T. Xia, H. Meng, C. Low-Kam, R. Liu, S. Pokhrel, S. Lin, X. Wang, Y.P. Liao, M. Wang, L. Li, R. Rallo, R. Damoiseaux, D. Telesca, L. Mädler, Y. Cohen, J.I. Zink, and A.E. Nel. 2012. Use of metal oxide nanoparticle band gap to develop a predictive paradigm for oxidative stress and acute pulmonary inflammation. ACS Nano 6 (5): 4349–4368.CrossRefPubMedPubMedCentralGoogle Scholar
  17. 17.
    Yuan, L., et al. 2017. Pirfenidone attenuates bleomycin-induced pulmonary fibrosis in mice by regulating Nrf2/Bach1 equilibrium. BMC Pulmonary Medicine 17 (1): 63.CrossRefGoogle Scholar
  18. 18.
    Yan, L., S.M. Yeligar, and A.S. Lou Brown. 2012. Chronic-alcohol-abuse-induced oxidative stress in the development of acute respiratory distress syndrome. TheScientificWorldJournal 2012 (2): 740308.Google Scholar
  19. 19.
    Miyazaki, T., et al. 2014. Video-assisted thoracic surgery attenuates perioperative oxidative stress response in lung cancer patients: a preliminary study. Acta Medica Nagasakiensia 59 (1): 19–25.Google Scholar
  20. 20.
    Xu, P., J. Xu, S. Liu, and Z. Yang. 2012. Nano copper induced apoptosis in podocytes via increasing oxidative stress. Journal of Hazardous Materials 241-242 (4): 279–286.CrossRefPubMedGoogle Scholar
  21. 21.
    SB, L., et al. 2017. Xanthotoxin suppresses LPS-induced expression of iNOS, COX-2, TNF-α, and IL-6 via AP-1, NF-κB, and JAK-STAT inactivation in RAW 264.7 macrophages. International Immunopharmacology 49: 21–29.CrossRefGoogle Scholar
  22. 22.
    Luo, K. 2017. Signaling cross talk between TGF-β/Smad and other signaling pathways. Cold Spring Harbor Perspectives in Biology 9 (1): a022137.CrossRefPubMedGoogle Scholar
  23. 23.
    Li, J., Y. Wei, X. Li, D. Zhu, B. Nie, J. Zhou, L. Lou, B. Dong, A. Wu, Y. Che, M. Chen, L. Zhu, M. Mu, and L. Chai. 2017. Herbal formula Xian-Fang-Huo-Ming-Yin regulates differentiation of lymphocytes and production of pro-inflammatory cytokines in collagen-induced arthritis mice. BMC Complementary and Alternative Medicine 17 (1): 12.CrossRefPubMedPubMedCentralGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of RespirationFirst Affiliated Hospital of Wenzhou Medical UniversityWenzhouPeople’s Republic of China

Personalised recommendations