Inflammation

, Volume 41, Issue 1, pp 114–121 | Cite as

Artesunate Inhibits Renal Ischemia Reperfusion-Stimulated Lung Inflammation in Rats by Activating HO-1 Pathway

  • Zhaohui Liu
  • Junjie Zhang
  • Shitong Li
  • Jihong Jiang
ORIGINAL ARTICLE
First Online:

ABSTRACT

Artesunate (AS), a semi-synthetic derivative of Artemisia, has been shown to exert a wide range of pharmacological effects, such as anti-inflammatory and antioxidant functions. However, the protective functions of AS on renal ischemia reperfusion injury (RIR)-stimulated lung inflammation remain unclear. In this research, acute lung injury (ALI) was stimulated by renal ischemia reperfusion injury (RIR). AS (15 mg/kg) was intraperitoneal administrated to rat 1 h before RIR stimulation. Serum and pulmonary NO, MDA, IL-6, MIP-2, and PGE2 levels, arterial blood gas and biochemistry, lung wet/dry weight ratio and MPO activity, total cell number and protein concentration in BALF, tissue histology, and NF-κB expression were determined. The results indicated that serum and pulmonary NO, MDA, IL-6, MIP-2, and PGE2 levels, lung wet/dry weight ratio and MPO activity, total cell number, and protein concentration in BALF enhanced after RIR stimulation. These alterations were mitigated by AS. AS attenuated lung wet/dry weight ratio and MPO activity, total cell number, and protein concentration in BALF. AS attenuated RIR-stimulated pulmonary NF-κB phosphorylation. In addition, these previously mentioned actions of AS were antagonized by suppressing HO-1 pathway. However, RIR-stimulated arterial blood gas and biochemistry and lung histopathology were also attenuated by AS. In summary, AS inhibited RIR-stimulated lung inflammation by activating HO-1 pathway.

KEY WORDS

artesunate renal ischemia reperfusion injury acute lung injury HO-1 NF-κB 
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Notes

Authors’ Contributions

Zhaohui Liu: conception, design, analysis and interpretation of data; writing the manuscript. Junjie Zhang: conception, design, analysis and interpretation of data; writing the manuscript. Shitong Li: analysis and interpretation of data; writing the manuscript. Jihong Jiang: conception, design, analysis and interpretation of data; writing the manuscript.

Compliance with Ethical Standards

All procedures were performed in accordance with the Declaration of Helsinki of the World Medical Association. The study was approved by the ethics committee of Shanghai First People’s Hospital, Shanghai, China.

Conflicts of Interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer Science+Business Media, LLC 2017

Authors and Affiliations

  1. 1.Department of Anesthesiology, Cangzhou Central HospitalCangzhouChina
  2. 2.Department of Anesthesiology, Shanghai First People’s HospitalShanghai Jiaotong University School of MedicineShanghaiChina

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