Obesity Exacerbates Sepsis-Induced Oxidative Damage in Organs
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Sepsis progression is linked to the imbalance between reactive oxygen species and antioxidant enzymes. Sepsis affects multiple organs, but when associated with a chronic inflammatory disease, such as obesity, it may be exacerbated. We hypothesized that obesity could aggravate the oxidative damage to peripheral organs of rats submitted to an animal model of sepsis. Male Wistar rats aged 8 weeks received hypercaloric nutrition for 2 months to induce obesity. Sepsis was induced by cecal ligation and puncture (CLP) procedure, and sham-operated rats were considered as control group. The experimental groups were divided into sham + eutrophic, sham + obese, CLP + eutrophic, and CLP + obese. Twelve and 24 h after surgery, oxidative damage to lipids and proteins and superoxide dismutase (SOD) and catalase (CAT) activities were evaluated in the liver, lung, kidney, and heart. The data indicate that obese rats subjected to sepsis present oxidative stress mainly in the lung and liver. This alteration reflected an oxidative damage to lipids and proteins and an imbalance of SOD and CAT levels, especially 24 h after sepsis. It follows that obesity due to its pro-inflammatory phenotype can aggravate sepsis-induced damage in peripheral organs.
KEY WORDSsepsis obesity organs oxidative stress inflammation
This research was supported by the Programa de Pós-graduação em Ciências da Saúde–UNISUL and the CNPq.
Compliance with Ethical Standards
All studies were performed in compliance with the National Institutes of Health Guidelines and with the approval of the Animal Care and Experimentation Committee of UNISUL (protocol number 13.026.4.03.IV), Brazil.
Conflict of Interest
The authors declare that they have no conflict of interest.
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