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Inflammation

, Volume 39, Issue 3, pp 1025–1038 | Cite as

Piperine Augments the Protective Effect of Curcumin Against Lipopolysaccharide-Induced Neurobehavioral and Neurochemical Deficits in Mice

  • Ashok JangraEmail author
  • Mohit Kwatra
  • Tavleen Singh
  • Rajat Pant
  • Pawan Kushwah
  • Yogita Sharma
  • Babita Saroha
  • Ashok Kumar Datusalia
  • Babul Kumar Bezbaruah
ORIGINAL ARTICLE

Abstract

The aim of the present study was to investigate the protective effects of curcumin alone and in combination with piperine against lipopolysaccharide (LPS)-induced neurobehavioral and neurochemical deficits in the mice hippocampus. Mice were treated with curcumin (100, 200, and 400 mg/kg, p.o.) and piperine (20 mg/kg, p.o.) for 7 days followed by LPS (0.83 mg/kg, i.p.) administration. Animals exhibited anxiety and depressive-like phenotype after 3 and 24 h of LPS exposure, respectively. LPS administration increased the oxido-nitrosative stress as evident by elevated levels of malondialdehyde, nitrite, and depletion of glutathione level in the hippocampus. Furthermore, we found raised level of pro-inflammatory cytokines (IL-1β and TNF-α) in the hippocampus of LPS-treated mice. Pretreatment with curcumin alleviated LPS-induced neurobehavioral and neurochemical deficits. Furthermore, co-administration of curcumin with piperine significantly potentiated the neuroprotective effect of curcumin. These results demonstrate that piperine enhanced the neuroprotective effect of curcumin against LPS-induced neurobehavioral and neurochemical deficits.

KEY WORDS

depression anxiety curcumin piperine oxido-nitrostative stress 

Notes

Acknowledgements

We would like to thank the Department of Pharmaceuticals, Ministry of Chemicals and Fertilizers, Government of India, for financial support. The authors are immensely thankful to the Institutional Level Biotech hub, NIPER Guwahati for providing technical support.

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Ashok Jangra
    • 1
    Email author
  • Mohit Kwatra
    • 1
  • Tavleen Singh
    • 1
  • Rajat Pant
    • 1
  • Pawan Kushwah
    • 1
  • Yogita Sharma
    • 1
  • Babita Saroha
    • 2
  • Ashok Kumar Datusalia
    • 3
  • Babul Kumar Bezbaruah
    • 4
  1. 1.Department of Pharmacology and ToxicologyLaboratory of Neuroscience, National Institute of Pharmaceutical Education and ResearchGuwahatiIndia
  2. 2.Department of BiotechnologyUniversity Institute of Engineering & Technology (UIET), Maharishi Dayanand UniversityRohtakIndia
  3. 3.National Brain Research CentreManesar, GurgaonIndia
  4. 4.Department of PharmacologyGauhati Medical CollegeGuwahatiIndia

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