Correlation Between the Expression of MicroRNA-301a-3p and the Proportion of Th17 Cells in Patients with Rheumatoid Arthritis
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Rheumatoid arthritis (RA) is characterized by chronic synovial inflammation and subsequent joint destruction. Previous studies have confirmed that Th17 cells play a critical role in the pathogenesis of RA. MicroRNA (miR)-301a-3p is a regulatory factor for Th17 cells differentiation that contributes to the pathogenesis of autoimmune diseases. The purposes of this study were to identify the alteration of Th17 cells and analyze the correlation between the expression of the miR-301a-3p and the proportion of Th17 cells in RA patients. The results showed that the frequency of Th17 cells and the expression of transcription factors (RORγt and STAT3) significantly increased in the peripheral blood mononuclear cells (PBMCs) from RA patients, and the associated proinflammatory cytokines were also upregulated. We also observed that the expression of protein inhibitor of activated STAT3 (PIAS3), the main cellular inhibitor of STAT3, was attenuated in RA patients and negatively correlated with the percentage of Th17 cells in RA. Interestingly, miR-301a-3p, an inhibitor of PIAS3 expression, was overexpressed in the PBMCs from RA patients and positively correlated with the frequency of Th17 cells in patients with RA. Taken together, these data indicated that miR-301a-3p and Th17 cells were augmented in peripheral blood, which may play an important role in the process of RA.
KEY WORDSrheumatoid arthritis Th17 cells microrna-301a
This work was financially supported through grants from the National Natural Science Foundation of China (grant no. 31170849, 31470881), Specialized Research Fund for the Doctoral Program of Higher School (grant no. 20133227110008), Health Department Foundation of Jiangsu Province (grant no. Z201312), Jiangsu Province “333” Project (grant no. BRA2015197), Summit of the Six Top Talents Program of Jiangsu Province (grant no. 2015-WSN-116), Jiangsu University Science Foundation (grant nos. 15JDG070, 11JDG093, FCJJ2015022), Jiangsu Key Laboratory of Laboratory Medicine Foundation (grant no. JSKLM-2014-010), and the Priority Academic Development Program of Jiangsu Higher Education Institutions.
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Conflict of Interest
The authors declare that they have no competing interests.
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