Inflammation

, Volume 39, Issue 1, pp 425–433 | Cite as

Activation of Neutrophils via IP3 Pathway Following Exposure to Demodex-Associated Bacterial Proteins

  • Fred McMahon
  • Nessa Banville
  • David A. Bergin
  • Christian Smedman
  • Staffan Paulie
  • Emer Reeves
  • Kevin Kavanagh
Original Article
First Online:

Abstract

Rosacea is a chronic inflammatory condition that predominantly affects the skin of the face. Sera from rosacea patients display elevated reactivity to proteins from a bacterium (Bacillus oleronius) originally isolated from a Demodex mite from a rosacea patient suggesting a possible role for bacteria in the induction and persistence of this condition. This work investigated the ability of B. oleronius proteins to activate neutrophils and demonstrated activation via the IP3 pathway. Activated neutrophils displayed increased levels of IP1 production, F-actin formation, chemotaxis, and production of the pro-inflammatory cytokines IL-1β and IL-6 following stimulation by pure and crude B. oleronius protein preparations (2 μg/ml), respectively. In addition, neutrophils exposed to pure and crude B. oleronius proteins (2 μg/ml) demonstrated increased release of internally stored calcium (Ca2+), a hallmark of the IP3 pathway of neutrophil activation. Neutrophils play a significant role in the inflammation associated with rosacea, and this work demonstrates how B. oleronius proteins can induce neutrophil recruitment and activation.

KEY WORDS

Bacillus Demodex inflammation neutrophils rosacea 
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Notes

Acknowledgments

F. McM is funded by the Programme in BioAnalysis and Therapeutics (BioAT) by the Higher Education Authority of Ireland through PRTLI V.

Compliance with Ethical Standards

Conflict of interest

S. Paulie is employed by, and part-owner of, Mabtech AB. C. Smedman is employed by Mabtech AB. The other authors (F.McMahon, N. Banville, D. Bergin, E. Reeves, K. Kavanagh) have no conflicts of interest to declare.

Supplementary material

10753_2015_264_MOESM1_ESM.doc (432 kb)
ESM 1 (DOC 432 kb)

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Fred McMahon
    • 1
  • Nessa Banville
    • 2
  • David A. Bergin
    • 2
  • Christian Smedman
    • 3
  • Staffan Paulie
    • 3
  • Emer Reeves
    • 2
  • Kevin Kavanagh
    • 1
  1. 1.Department of BiologyMaynooth UniversityCo. KildareIreland
  2. 2.Respiratory Research Division, Department of Medicine, Royal College of Surgeons in IrelandBeaumont HospitalDublin 9Ireland
  3. 3.Mabtech ABNacka StrandSweden

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