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Inflammation

, Volume 39, Issue 1, pp 336–346 | Cite as

Effects of Administration of Amlodipine and Lacidipine on Inflammation-Induced Bone Loss in the Ovariectomized Rat

  • Emre KarakusEmail author
  • Zekai Halici
  • Abdulmecit Albayrak
  • Yasin Bayir
  • Elif Demirci
  • Ali Aydin
  • Berna Ozturk-Karagoz
  • Elif Cadirci
  • Arif Kursat Ayan
  • Ali Sahin
  • Deniz Unal
Original Article

Abstract

This study was performed to evaluate the possible protective effect of two calcium channel blocker’s “lacidipine (LAC) and amlodipine (AML)” on bone metabolism in an experimental ovariectomized and inflammation-induced osteoporosis rat model (OVXinf). For the purpose of this study, the rats were divided into eight groups, each containing eight rats: sham-operated control (group 1, SH), sham + inflammation (group 2, SHinf), ovariectomy (group 3, OVX), ovariectomy + inflammation (group 4, OVXinf), ovariectomy + LAC 4 mg/kg (group 5, OVX + LAC), ovariectomy + inflammation + LAC 4 mg/kg (group 6, OVXinf + LAC), ovariectomy + AML 5 mg/kg (group 7, OVX + AML), ovariectomy + inflammation + AML 5 mg/kg (group 8, OVXinf + AML). The levels of osteocalcin and osteopontin decreased in OVXinf + LAC and OVXinf + AML groups. The serum levels of TNF-α, IL-1β, and IL-6 were increased significantly in the OVXinf rats compared with the SH group. Gene expression levels of the osteogenic factor runt-related transcription factor 2 (Runx2) and type I collagen 1A1 (Col1A1) significantly decreased in the OVXinf group, when compared with the control group. AML or LAC administrations increased the levels of Runx2 and Col1A1. These results suggest that amlodipine and lacidipine may be a novel therapeutic target for radical osteoporosis treatment in hypertensive patients.

KEY WORDS

amlodipine lacidipine bone mineral density ovariectomized rat inflammation 

Notes

Acknowledgments

This article supported by TUBİTAK, project number 112S044, and Ataturk University Scientific Experimental Project Office, project number 2011/017.

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Emre Karakus
    • 1
    Email author
  • Zekai Halici
    • 2
  • Abdulmecit Albayrak
    • 2
  • Yasin Bayir
    • 3
  • Elif Demirci
    • 4
  • Ali Aydin
    • 5
  • Berna Ozturk-Karagoz
    • 6
  • Elif Cadirci
    • 2
  • Arif Kursat Ayan
    • 7
  • Ali Sahin
    • 7
  • Deniz Unal
    • 8
  1. 1.Department of Pharmacology and Toxicology, Faculty of Veterinary MedicineAtaturk UniversityErzurumTurkey
  2. 2.Department of Pharmacology, Faculty of MedicineAtaturk UniversityErzurumTurkey
  3. 3.Department of Biochemistry, Faculty of PharmacyAtaturk UniversityErzurumTurkey
  4. 4.Department of Pathology, Faculty of MedicineAtaturk UniversityErzurumTurkey
  5. 5.Department of Orthopedics and TraumatologyAtaturk University Faculty of MedicineErzurumTurkey
  6. 6.Department of Pharmacology, Faculty of PharmacyIbrahim Cecen UniversityAgrıTurkey
  7. 7.Department of Nuclear Medicine, Faculty of MedicineAtaturk UniversityErzurumTurkey
  8. 8.Department of Histology and Embryology, Faculty of MedicineAtaturk UniversityErzurumTurkey

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