Inflammation

, Volume 39, Issue 1, pp 107–112 | Cite as

Identification of Rab6a as a New Target of microRNA-155 Involved in Regulating Lipopolysaccharide-Induced TNF Secretion

Article
First Online:

Abstract

This study aims to provide experimental proof that Rab6a is an efficient target of microRNA-155 in regulating pro-inflammatory tumor necrosis factor (TNF) secretion stimulated by lipopolysaccharide (LPS) in macrophages. We identified Rab6a as a new target of microRNA-155 (miR-155) and found that overexpression of miR-155 decreased Rab6a expression at both protein and mRNA levels, which resulted in a significant reduction of TNF secretion induced by lipopolysaccharide stimulation. We have demonstrated that miR-155 can negatively regulate inflammatory TNF secretion in lipopolysaccharide stimulated macrophages, partly by targeting Rab6a, thereby providing new insights into the role of miR-155 in cytokine secretion in inflammatory macrophages.

KEY WORDS

microRNA-155 Rab6a inflammatory TNF LPS 
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Notes

Acknowledgments

We would like to thank all members of the Cardiology Department of Kunming General Hospital, Kunming, China; and the Medical Research Center of the Southwest Hospital of the Third Military Medical University, Sichuan, China for their assistance. We also thank Professor Jiang Zheng for the equipment support.

Conflict of Interest

The authors declare that they have no competing interests.

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  1. 1.Department of PostgraduateThird Military Medical UniversityChongqingChina
  2. 2.Department of CardiologyKunming General Hospital of Chengdu Military AreaYunnanChina

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