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Inflammation

, Volume 38, Issue 6, pp 2259–2268 | Cite as

Anti-inflammation Effects of Oxysophoridine on Cerebral Ischemia–Reperfusion Injury in Mice

  • Yong-Sheng Wang
  • Yu-Xiang Li
  • Peng Zhao
  • Hong-Bo Wang
  • Ru Zhou
  • Yin-Ju Hao
  • Jie Wang
  • Shu-Jing Wang
  • Juan Du
  • Lin Ma
  • Tao Sun
  • Jian-Qiang YuEmail author
Article

Abstract

Oxysophoridine (OSR) is a bioactive alkaloid extracted from the Sophora alopecuroides Linn. Our aim is to explore the potential anti-inflammation mechanism of OSR in cerebral ischemic injury. Mice were intraperitoneally pretreated with OSR (62.5, 125, and 250 mg/kg) or nimodipine (Nim) (6 mg/kg) for 7 days followed by cerebral ischemia. The inflammatory-related cytokines in cerebral ischemic hemisphere tissue were determined by immunohistochemistry staining, Western blot and enzyme-like immunosorbent assay (ELISA). OSR-treated groups observably suppressed the nuclear factor kappa B (NF-κB), intercellular adhesion molecule-1 (ICAM-1), inducible nitric oxide synthase (iNOS), and cyclooxygenase-2 (COX-2). OSR-treated group (250 mg/kg) markedly reduced the inflammatory-related protein prostaglandin E2 (PGE2), tumor necrosis factor alpha (TNF-α), interleukin-1β (IL-1β), interleukin-6 (IL-6), and interleukin-8 (IL-8). Meanwhile, it dramatically increased the interleukin-10 (IL-10). Our study revealed that OSR protected neurons from ischemia-induced injury in mice by downregulating the proinflammatory cytokines and blocking the NF-κB pathway.

KEY WORDS

oxysophoridine anti-inflammation cerebral ischemia NF-κB 

Notes

Acknowledgments

We are grateful to Dr. Margaret for editing and polishing the manuscript. The study was supported by the National Natural Science Foundation of China (Grant No. 309605060) and the Natural Science Foundation of Ningxia (Grant No. NZ11212).

Conflict of Interest

There is no conflict of interest.

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Yong-Sheng Wang
    • 1
  • Yu-Xiang Li
    • 2
  • Peng Zhao
    • 1
  • Hong-Bo Wang
    • 1
  • Ru Zhou
    • 1
  • Yin-Ju Hao
    • 3
  • Jie Wang
    • 4
  • Shu-Jing Wang
    • 4
  • Juan Du
    • 1
  • Lin Ma
    • 5
  • Tao Sun
    • 5
  • Jian-Qiang Yu
    • 1
    • 6
    • 7
    Email author
  1. 1.Department of PharmacologyNingxia Medical UniversityYinchuanChina
  2. 2.College of NursingNingxia Medical UniversityYinchuanChina
  3. 3.Ningxia Medical UniversityYinchuanChina
  4. 4.Medical Sci-Tech Research CenterNingxia Medical UniversityYinchuanChina
  5. 5.Ningxia Key Lab of Craniocerebral Diseases of Ningxia Hui Autonomous RegionYinchuanChina
  6. 6.Ningxia Hui Medicines Collaborative Innovation CenterYinchuanChina
  7. 7.Department of PharmacologyNingxia Medical University and Ningxia Hui Medicines Collaborative Innovation CenterYinchuanChina

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