, Volume 38, Issue 5, pp 1986–1995 | Cite as

The Neuroprotective Effect of Coumaric Acid on Spinal Cord Ischemia/Reperfusion Injury in Rats

  • Mustafa GuvenEmail author
  • Muserref Hilal Sehitoglu
  • Yasemin Yuksel
  • Mehmet Tokmak
  • Adem Bozkurt Aras
  • Tarik Akman
  • Umut Hatay Golge
  • Ergun Karavelioglu
  • Ercan Bal
  • Murat Cosar


The main causes of spinal cord ischemia are a variety of vascular pathologies causing acute arterial occlusions. We investigated neuroprotective effects of coumaric acid on spinal cord ischemia injury in rats. Rats were divided randomly into four groups of eight animals as follows: control, ischemia, ischemia + coumaric acid, and ischemia + methylprednisolone. In the control group, only a laparotomy was performed. In all other groups, the spinal cord ischemia was performed by the infrarenal aorta cross-clamping model. Levels of malondialdehyde and nuclear respiratory factor 1 were analyzed, as were the activity of superoxide dismutase. Histopathological and immunohistochemical evaluations were performed. Neurological evaluation was performed with the Tarlov scoring system. The ischemia + coumaric acid group was compared with the ischemia group, and a significant decrease in malondialdehyde and levels was observed. Nuclear respiratory factor 1 level and superoxide dismutase activity of the ischemia + coumaric acid group were significantly higher than in the ischemia group. In histopathological samples, the ischemia + coumaric acid group is compared with the ischemia group, and there was a significant increase in numbers of normal neurons. In immunohistochemical staining, hypoxia-inducible factor-1α and NF-kappa B immunopositive neurons were significantly decreased in the ischemia + coumaric acid group compared with that in the ischemia group. The neurological deficit scores of the ischemia + coumaric acid group were significantly higher than the ischemia group at 24 h. Our results revealed for the first time that coumaric acid exhibits meaningful neuroprotective activity following ischemia-reperfusion injury of the spinal cord.


coumaric acids spinal cord ischemia hypoxia-inducible factor 1 NF-kappa B 


Conflict of Interest

The authors declare that they have no competing interests and financial disclosure.


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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Mustafa Guven
    • 1
    Email author
  • Muserref Hilal Sehitoglu
    • 2
  • Yasemin Yuksel
    • 3
  • Mehmet Tokmak
    • 4
  • Adem Bozkurt Aras
    • 1
  • Tarik Akman
    • 1
  • Umut Hatay Golge
    • 5
  • Ergun Karavelioglu
    • 6
  • Ercan Bal
    • 7
  • Murat Cosar
    • 1
  1. 1.Department of Neurosurgery, Faculty of MedicineCanakkale Onsekiz Mart UniversityCanakkaleTurkey
  2. 2.Department of Medical Biochemistry, Faculty of MedicineCanakkale Onsekiz Mart UniversityCanakkaleTurkey
  3. 3.Department of Histology & Embryology, Faculty of MedicineAfyon Kocatepe UniversityAfyonTurkey
  4. 4.Department of Neurosurgery, Faculty of MedicineMedipol UniversityIstanbulTurkey
  5. 5.Department of Orthopaedics, Faculty of MedicineCanakkale Onsekiz Mart UniversityCanakkaleTurkey
  6. 6.Department of Neurosurgery, Faculty of MedicineAfyon Kocatepe UniversityAfyonTurkey
  7. 7.Department of NeurosurgeryAtaturk Education and Research HospitalAnkaraTurkey

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