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Inflammation

, Volume 38, Issue 5, pp 1925–1932 | Cite as

Artesunate Attenuates Lipopolysaccharide-Stimulated Proinflammatory Responses by Suppressing TLR4, MyD88 Expression, and NF-κB Activation in Microglial Cells

  • Dunjing Wang
  • Jun Shi
  • Shuiqing Lv
  • Weiwei Xu
  • Jizhen Li
  • Wei Ge
  • Chenghua Xiao
  • Deqin GengEmail author
  • Yonghai LiuEmail author
Article

Abstract

Microglia are considered as a major target in the prevention of neuroinflammation by modulating the production of pro-inflammatory mediators. Artesunate, a water-soluble artemisinin derivative, exerts an anti-inflammatory effect. In the present study, we showed artesunate dose-dependently suppressed the lipopolysaccharide (LPS)-induced production of nitric oxide (NO), inducible nitric oxide synthase (iNOS), and interleukin-1beta (IL-1β) in BV2 microglial cells. In addition, artesunate inhibited LPS-induced expression of Toll-like receptor 4 (TLR4), myeloid differentiation factor 88 (MyD88), and activation of nuclear factor kappa B (NF-κB) by blockade of inhibitor of NF-κB (IκB) degradation. This data indicate that artesunate attenuates the generation of proinflammatory mediators on LPS-stimulated BV-2 microglial cells. And this effect may be associated with the suppression of TLR4/MyD88/NF-κB signaling pathways. Therefore, artesunate may be a potential anti-neuroinflammatory agent.

KEY WORDS

artesunate neuroinflammation microglia Toll-like receptor 4 NF-κB 

Notes

Acknowledgments

This research was funded by the affiliated hospital of Xuzhou Medical College (No. 2013104049). Additional support is provided by the neurobiology laboratory of Xuzhou Medical College.

Conflict of Interest

There is no conflict of interest to disclose.

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Dunjing Wang
    • 1
  • Jun Shi
    • 2
    • 3
  • Shuiqing Lv
    • 2
  • Weiwei Xu
    • 2
  • Jizhen Li
    • 4
  • Wei Ge
    • 1
  • Chenghua Xiao
    • 1
  • Deqin Geng
    • 1
    Email author
  • Yonghai Liu
    • 1
    Email author
  1. 1.Department of NeurologyAffiliated Hospital of Xuzhou Medical CollegeXuzhouChina
  2. 2.Postgraduate SchoolXuzhou Medical CollegeXuzhouChina
  3. 3.Department of NeurologyLiyang People’s HospitalLiyangChina
  4. 4.Department of NeurologySuzhou Kowloon Hospital of Shanghai Jiaotong University Medical SchoolSuzhouChina

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