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Inflammation

, Volume 37, Issue 6, pp 2047–2055 | Cite as

Oxymatrine Lightened the Inflammatory Response of LPS-Induced Mastitis in Mice Through Affecting NF-κB and MAPKs Signaling Pathways

  • Zhengtao Yang
  • Ronglan Yin
  • Yunfeng Cong
  • Zhanqing Yang
  • Ershun Zhou
  • Zhengkai Wei
  • Zhicheng Liu
  • Yongguo Cao
  • Naisheng ZhangEmail author
Article

Abstract

Mastitis, an inflammatory reaction of the mammary gland, is recognized as one of the most costly diseases in dairy cattle. Oxymatrine, one of the alkaloids extracted from Chinese herb Sophora flavescens Ait, has been reported to have many biological activities, such as anti-inflammatory, anti-virus, and anti-hepatic fibrosis properties. The aim of this study was to investigate the protective effect and the anti-inflammatory mechanism of oxymatrine on lipopolysaccharide (LPS)-induced mastitis in mice. The mouse mastitis was induced by 10 μg of LPS for 24 h. Oxymatrine was intraperitoneally administered with the dose of 30, 60, and 120 mg/kg 1 h before and 12 h after LPS induction. The results showed that oxymatrine significantly attenuated the damage of the mammary gland induced by LPS. Oxymatrine inhibited the phosphorylation of NF-κB p65 and IκB in NF-κB signal pathway and reduced the phosphorylation of p38, ERK, and JNK in mitogen-activated protein kinase (MAPKs) signal pathway. The results showed that oxymatrine had a protective effect on LPS-induced mastitis, and the anti-inflammatory mechanism of oxymatrine was related to the inhibition of NF-κB and MAPKs signal pathways.

KEY WORDS

oxymatrine LPS mastitis NF-κB MAPKs 

Notes

Acknowledgments

This work was supported by a grant from the National Natural Science Foundation of China (No. 31272622, 31201926).

Conflict of Interests

The authors declare that they have no conflicts of interests.

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Zhengtao Yang
    • 1
  • Ronglan Yin
    • 2
  • Yunfeng Cong
    • 3
  • Zhanqing Yang
    • 1
  • Ershun Zhou
    • 1
  • Zhengkai Wei
    • 1
  • Zhicheng Liu
    • 1
  • Yongguo Cao
    • 1
  • Naisheng Zhang
    • 1
    Email author
  1. 1.Department of Clinical Veterinary Medicine, College of Veterinary MedicineJilin UniversityChangchunPeople’s Republic of China
  2. 2.Research Academy of Animal Husbandry and Veterinary Medicine Sciences of Jilin ProvinceChangchunPeople’s Republic of China
  3. 3.Department of Gynecology, The First Hospital of Jilin UniversityJilin UniversityChangchunPeople’s Republic of China

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