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Inflammation

, Volume 37, Issue 6, pp 1976–1983 | Cite as

The Effects of Morin on Lipopolysaccharide-Induced Acute Lung Injury by Suppressing the Lung NLRP3 Inflammasome

  • Zhang TianzhuEmail author
  • Yang Shihai
  • Du Juan
Article

Abstract

In previous study, the anti-inflammatory effect of morin had been found. In this study, we investigated anti-inflammatory effects of morin on acute lung injury using lipopolysaccharide (LPS)-induced acute lung injury (ALI) mouse model. The cell counting in the bronchoalveolar lavage fluid (BALF) was measured. The animal lung edema degree was evaluated by wet/dry weight (W/D) ratio. The superoxidase dismutase (SOD) activity and myeloperoxidase (MPO) activity were assayed by SOD and MPO kits, respectively. The levels of inflammatory mediators including tumor necrosis factor-α (TNF-α), interleukin (IL)-1β, IL-18, and IL-6 were assayed by enzyme-linked immunosorbent assay method. Pathological changes of lung tissues were observed by hematoxylin and eosin (HE) staining. The protein level of lung NACHT, LRR, and PYD domains-containing protein 3 (NLRP3) inflammasome was measured by Western blotting. The data showed that treatment with the morin markedly attenuated inflammatory cell numbers in the BALF, decreased lung NLRP3 inflammasome protein level, and improved SOD activity and inhibited MPO activity. Histological studies demonstrated that morin substantially inhibited LPS-induced neutrophils in lung tissue compared with model group. The results indicated that the morin had a protective effect on LPS-induced ALI in mice.

KEY WORDS

morin NLRP3 inflammasome LPS-induced acute lung injury 

Notes

Acknowledgments

This work was supported by a project funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions.

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  1. 1.School of Pharmaceutical Sciences Changchun University of Chinese MedicineChanghcunChina
  2. 2.College of Chinese Medicinal Materials, Jilin Agricultural UniversityChangchunChina
  3. 3.School of Life SciencesPeking UniversityBeijingChina

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