, Volume 37, Issue 5, pp 1837–1846 | Cite as

Stevioside Plays an Anti-inflammatory Role by Regulating the NF-κB and MAPK Pathways in S. aureus-infected Mouse Mammary Glands

  • Tiancheng Wang
  • Mengyao Guo
  • Xiaojing Song
  • Zecai Zhang
  • Haichao Jiang
  • Wei Wang
  • Yunhe Fu
  • Yongguo Cao
  • Lianqin Zhu
  • Naisheng Zhang


Mastitis is an inflammatory disease caused by microbial infection. Staphylococcus aureus is one of the primary bacteria responsible for mastitis. Stevioside is isolated from Stevia rebaudiana and is known to have therapeutic functions. This study was designed to evaluate the effects of stevioside in a mouse model of S. aureus-induced mastitis. In this study, the mouse mammary gland was infected with S. aureus to induce the mastitis model. The stevioside was administered intraperitoneally after the S. aureus infection was established. Hematoxylin–eosin (HE) staining, ELISA, Western blot, and q-PCR methods were used. The results show that stevioside significantly reduced the inflammatory cell infiltration and the levels of TNF-α, IL-1β, and IL-6 and the respective expression of their messenger RNAs (mRNAs). Further studies revealed that stevioside downregulated the TLR2, NF-κB, and (mitogen-activated protein kinase) MAPK signaling pathways in the S. aureus-infected mouse mammary gland. Our results demonstrate that stevioside reduced the expression of TNF-α, IL-1β, and IL-6 by inhibiting the phosphorylation of proteins in the NF-κB and MAPK signaling pathways dose-dependently, but that their mRNA expression was not obviously changed.


stevioside TLR2 anti-inflammatory NF-κB MAPK 



This work was supported by a grant from the National Natural Science Foundation of China (Nos. 31272622, 31201925), the Research Fund for the Doctoral Program of Higher Education of China (Nos. 20110061130010, 20120061120098), and Jilin Province Science Foundation for Youths (No. 20130522087JH).


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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Tiancheng Wang
    • 1
  • Mengyao Guo
    • 1
  • Xiaojing Song
    • 1
  • Zecai Zhang
    • 1
  • Haichao Jiang
    • 1
  • Wei Wang
    • 1
  • Yunhe Fu
    • 1
  • Yongguo Cao
    • 1
  • Lianqin Zhu
    • 2
  • Naisheng Zhang
    • 1
  1. 1.Department of Clinical Veterinary MedicineCollege of Veterinary Medicine, Jilin UniversityChangchunPeople’s Republic of China
  2. 2.College of Animal Science and TechnologyQingdao Agriculture UniversityQingdaoPeople’s Republic of China

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