Inflammation

, Volume 37, Issue 5, pp 1504–1512 | Cite as

Differential Association of S100A9, an Inflammatory Marker, and p53, a Cell Cycle Marker, Expression with Epicardial Adipocyte Size in Patients with Cardiovascular Disease

  • Rosa María Agra
  • Ángel Fernández-Trasancos
  • Juan Sierra
  • José Ramón González-Juanatey
  • Sonia Eiras
Article

ABSTRACT

S100A9 (calgranulin B) has inflammatory and oxidative stress properties and was found to be associated with atherosclerosis and obesity. One of the proteins that can regulate S100A9 transcription is p53, which is involved in cell cycle, apoptosis and adipogenesis. Thus, it triggers adipocyte enlargement and finally obesity. Because epicardial adipose tissue (EAT) volume and thickness is related to coronary artery disease (CAD), we studied the gene expression of this pathway in patients with cardiovascular disease and its association with obesity. Adipocytes and stromal cells from EAT and subcutaneous adipose tissue (SAT) from 48 patients who underwent coronary artery bypass graft and/or valve replacement were obtained after collagenase digestion and differential centrifugation. The expression levels of the involved genes on adipogenesis and cell cycle like fatty acid-binding protein (FABP) 4, retinol-binding protein (RBP)4, p53 and S100A9 were determined by real-time polymerase chain reaction (PCR). Adipocyte diameter was measured by optical microscopy. We found that epicardial adipocytes expressed significantly lower levels of adipogenic genes (FABP4 and RBP4) and cell cycle-related genes (S100A9 and p53) than subcutaneous adipocytes. However, in obese patients, upregulation of adipogenic and cell cycle-related genes in subcutaneous and epicardial adipocytes, respectively, was observed. The enlargement of adipocyte size was related to FABP4, S100A9 and p53 expression levels in stromal cells. But only the p53 association was maintained in epicardial stromal cells from obese patients (p = 0.003). The expression of p53, but not S100A9, in epicardial stromal cells is related to adipocyte enlargement in obese patients with cardiovascular disease. These findings suggest new mechanisms for understanding the relationship between epicardial fat thickness, obesity and cardiovascular disease.

KEY WORDS

S100A9 p53 epicardial adipose tissue adipocytes 

Supplementary material

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ESM 1(JPEG 52 kb)

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Rosa María Agra
    • 1
  • Ángel Fernández-Trasancos
    • 2
  • Juan Sierra
    • 3
  • José Ramón González-Juanatey
    • 1
    • 2
  • Sonia Eiras
    • 2
  1. 1.Department of Cardiology and Coronary UnitClinical Hospital of Santiago de CompostelaSantiago de CompostelaSpain
  2. 2.Health Research InstituteClinical Hospital of Santiago de CompostelaSantiago de CompostelaSpain
  3. 3.Department of Cardiac SurgeryClinical Hospital of Santiago de CompostelaSantiago de CompostelaSpain

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