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Inflammation

, Volume 37, Issue 2, pp 358–364 | Cite as

Protective Effect of p-Cymene on Lipopolysaccharide-Induced Acute Lung Injury in Mice

  • Lvyi Chen
  • Lei Zhao
  • Chunfeng ZhangEmail author
  • Zhou LanEmail author
Article

Abstract

In the previous study, the anti-inflammatory effect of p-cymene had been found. In this study, we investigated anti-inflammatory effects of p-cymene on acute lung injury using lipopolysaccharide (LPS)-induced acute lung injury (ALI) mouse model. The cell counting in the bronchoalveolar lavage fluid (BALF) was measured. The animal lung edema degree was evaluated by wet/dry weight (W/D) ratio. The superoxidase dismutase (SOD) activity and myeloperoxidase (MPO) activity was assayed by SOD and MPO kits, respectively. The levels of inflammatory mediators including tumor necrosis factor alpha (TNF-α), IL-1β, and IL-6 were assayed by enzyme-linked immunosorbent assay method. The pathological changes of the lung tissues were observed by hematoxylin and eosin staining. The inflammatory signal pathway-related protein levels of NF-κB were measured using Western blotting. The data showed that treatment with the p-cymene markedly attenuated inflammatory cell numbers in the BALF, decreased NF-κB protein level in the lungs, improved SOD activity, and inhibited MPO activity. Histological studies demonstrated that p-cymene substantially inhibited LPS-induced neutrophils in the lung tissue compared with the model group. The results indicated that p-cymene had a protective effect on LPS-induced ALI in mice.

KEY WORDS

p-cymene inflammation LPS-induced acute lung injury NF-κB 

Notes

Acknowledgments

The project was supported by Chinese National Project of “Twelfth Five-Year” Plan for Science & Technology Support (2012BAI27B06), the Special Fund for Basic Scientific Research of Central Colleges, South-Central University for Nationalities (no. CZY13016), the Natural Science Foundation of China (81303284) and the Planning of Deeply Participating into the Enterprises for Young Teachers (XD2012060).

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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  1. 1.College of PharmacySouth-Central University for NationalitiesWuhanPeople’s Republic of China
  2. 2.Key Laboratory of Chemistry and Quality for Traditional Chinese Medicines of the College of Gansu ProvinceGansu College of Traditional Chinese MedicineLanzhouPeople’s Republic of China
  3. 3.Key Laboratory of Modern Chinese Medicines, China Pharmaceutical UniversityMinistry of EducationNanjingPeople’s Republic of China
  4. 4.School of PharmacyHubei University of Chinese MedicineWuhanPeople’s Republic of China

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