, Volume 36, Issue 3, pp 616–624

Zinc Sulfate Inhibited Inflammation of Der p2-Induced Airway Smooth Muscle Cells by Suppressing ERK1/2 and NF-κB Phosphorylation



Inflammation of airway smooth muscle cells (ASMCs) is believed to be important in causing airway hyperresponsiveness. However, zinc has been reported to be implicated in many kinds of cell inflammation. Little is known about the effect of zinc treatment on Der p2 (group II Dermatophagoides pteronyssinus)-induced inflammation from ASMCs. This study investigated effects and mechanisms of zinc in Der p2-treated ASMCs. Der p2-treated primary ASMCs were cultured with various concentrations of zinc sulfate (ZnSO4) 6 μM, 12 μM, 24 μM, and 96 μM. The proteins and mRNAs of cytokines in ASMCs were examined by ELISA and real-time PCR. Intracellular zinc was stained with Zinquin fluorescence. The cell signaling protein expression was detected by Western blot. Der p2 was used to induce interleukin (IL)-6, IL-8, IL-1, and monocyte chemotactic protein-1 production of ASMCs. However, we found that 24 μM ZnSO4 reduced these inflammatory mediators production of Der p2-treated primary ASMCs. Der p2-induced extracellular signal-regulated kinases (ERK) and nuclear factor-kappa B (NF-κB) phosphorylation were suppressed by supplementation of 24 μM ZnSO4. Zinc is an anti-inflammatory agent that reduces inflammation of Der p2-treated ASMCs through the suppression of the ERK and NF-κB pathway. The results may be helpful for the development of effective treatments.


zinc asthma airway smooth muscle cells Der p2 inflammation 



Airway smooth muscle cells


Airway hyperresponsiveness

Der p2

Group II Dermatophagoides pteronyssinus


Extracellular signal-regulated kinases


Nuclear factor-kappa B


Hanks’ balanced salt solution


Dulbecco’s modified Eagle’s medium


Fetal bovine serum




Monocyte chemotactic protein


House dust mite


Bovine serum albumin


Fetal bovine serum


Sodium dodecyl sulfate


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Copyright information

© Springer Science+Business Media New York 2012

Authors and Affiliations

  1. 1.Department of Medical Research, Kuang Tien General HospitalTaichungRepublic of China
  2. 2.Department of Nutrition & Institute of BioMedical NutritionHungkuang UniversityTaichungRepublic of China

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