, Volume 36, Issue 2, pp 372–378 | Cite as

Inhibitory Mechanism of 10-Hydroxy-trans-2-decenoic Acid (Royal Jelly Acid) Against Lipopolysaccharide- and Interferon-β-Induced Nitric Oxide Production

  • Tsuyoshi SugiyamaEmail author
  • Keita Takahashi
  • Akihiro Kuzumaki
  • Shunji Tokoro
  • Paola Neri
  • Hiroshi Mori


Royal jelly acid, 10-hydroxy-trans-2-decenoic acid (10H2DA), is a major lipid component of royal jelly, which is the exclusive diet of queen honeybees. Previously, we showed partial inhibition of lipopolysaccharide (LPS)-induced NF-κB activation by 10H2DA. In this study, the ability of 10H2DA to inhibit LPS-induced nitric oxide (NO) production was investigated. LPS-induced NO production and inducible NO synthase (iNOS) gene transcription were inhibited by 10H2DA. LPS-stimulated interferon (IFN)-β production, IFN regulatory factor-1 induction and IFN-stimulated response element activation, which are required for iNOS induction, were unaffected by 10H2DA. IFN-β-induced NO production, however, was significantly inhibited by 10H2DA. Furthermore, IFN-β-induced nuclear factor (NF)-κB activation and tumour necrosis factor (TNF)-α production were significantly inhibited by 10H2DA, and TNF-α-induced NF-κB activation was also inhibited by 10H2DA. These results and our previous study suggest that 10H2DA inhibits LPS- and IFN-β-induced NO production via inhibition of NF-κB activation induced by LPS or IFN-β.


fatty acid lipopolysaccharide nitric oxide royal jelly 



This work was supported by a grant from the Japan Royal Jelly Fair Trade Council (Tokyo, Japan).

Conflict of Interest

The authors have no conflict of interest to declare.

Supplementary material

10753_2012_9556_MOESM1_ESM.doc (56 kb)
ESM 1 (DOC 56 kb)
10753_2012_9556_MOESM2_ESM.ppt (2.2 mb)
ESM 2 (PPT 2236 kb)


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Copyright information

© Springer Science+Business Media New York 2012

Authors and Affiliations

  • Tsuyoshi Sugiyama
    • 1
    Email author
  • Keita Takahashi
    • 1
  • Akihiro Kuzumaki
    • 1
  • Shunji Tokoro
    • 1
  • Paola Neri
    • 1
  • Hiroshi Mori
    • 1
  1. 1.Department of Biopharmaceutical Sciences, Laboratory of MicrobiologyGifu Pharmaceutical UniversityGifuJapan

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