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Protective Effect of Magnolol on Lipopolysaccharide-Induced Acute Lung Injury in Mice

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Abstract

Magnolol, a tradition Chinese herb, displays an array of activities including antifungal, antibacterial, and antioxidant effects. To investigate the protective effect of magnolol on lipopolysaccharide (LPS)-induced acute lung injury (ALI) in mice. ALI was induced in mice by intratracheal instillation of LPS (1 mg/kg). The mice received intratracheal instillation of magnolol (5 μg/kg) 30 min before LPS administration. Pulmonary histological changes were evaluated by hematoxylin-eosin stain and lung wet/dry weight ratios were observed. Concentrations of tumor necrosis factor (TNF)-α and interleukin (IL)-1β, and myeloperoxidase (MPO) activity were measured by enzyme-linked immunosorbent assay. Expression of cyclooxygenase (COX)-2 in lung tissues was determined by Western blot analysis. Magnolol pretreatment significantly attenuated the severity of lung injury and inhibited the production of TNF-α and IL-1β in mice with ALI. After LPS administration, the lung wet/dry weight ratios, as an index of lung edema, and MPO activity were also markedly reduced by magnolol pretreatment. The expression of COX-2 was significantly suppressed by magnolol pretreatment. Magnolol potently protected against LPS-induced ALI and the protective effects of magnolol may attribute partly to the suppression of COX-2 expression.

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Authors and Affiliations

  1. Department of Thoracic Surgery, Tangdu Hospital, The Fourth Military Medical University, Xi’an, 710038, China

    Yun Feng Ni, Tao Jiang, Qing Shu Cheng, Zhong Ping Gu, Yi Fang Zhu, Zhi Pei Zhang, Jian Wang, Xiao Long Yan, Wu Ping Wang, Chang Kang Ke, Yong Han & Xiao Fei Li

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  1. Yun Feng Ni
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  2. Tao Jiang
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  3. Qing Shu Cheng
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Correspondence to Yong Han or Xiao Fei Li.

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Yun Feng Ni and Tao Jiang authors contributed equally to this work and should be regarded as co-first authors.

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Ni, Y.F., Jiang, T., Cheng, Q.S. et al. Protective Effect of Magnolol on Lipopolysaccharide-Induced Acute Lung Injury in Mice. Inflammation 35, 1860–1866 (2012). https://doi.org/10.1007/s10753-012-9507-9

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  • DOI: https://doi.org/10.1007/s10753-012-9507-9

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