Inflammation

, Volume 35, Issue 6, pp 1860–1866 | Cite as

Protective Effect of Magnolol on Lipopolysaccharide-Induced Acute Lung Injury in Mice

  • Yun Feng Ni
  • Tao Jiang
  • Qing Shu Cheng
  • Zhong Ping Gu
  • Yi Fang Zhu
  • Zhi Pei Zhang
  • Jian Wang
  • Xiao Long Yan
  • Wu Ping Wang
  • Chang Kang Ke
  • Yong Han
  • Xiao Fei Li
Article

Abstract

Magnolol, a tradition Chinese herb, displays an array of activities including antifungal, antibacterial, and antioxidant effects. To investigate the protective effect of magnolol on lipopolysaccharide (LPS)-induced acute lung injury (ALI) in mice. ALI was induced in mice by intratracheal instillation of LPS (1 mg/kg). The mice received intratracheal instillation of magnolol (5 μg/kg) 30 min before LPS administration. Pulmonary histological changes were evaluated by hematoxylin-eosin stain and lung wet/dry weight ratios were observed. Concentrations of tumor necrosis factor (TNF)-α and interleukin (IL)-1β, and myeloperoxidase (MPO) activity were measured by enzyme-linked immunosorbent assay. Expression of cyclooxygenase (COX)-2 in lung tissues was determined by Western blot analysis. Magnolol pretreatment significantly attenuated the severity of lung injury and inhibited the production of TNF-α and IL-1β in mice with ALI. After LPS administration, the lung wet/dry weight ratios, as an index of lung edema, and MPO activity were also markedly reduced by magnolol pretreatment. The expression of COX-2 was significantly suppressed by magnolol pretreatment. Magnolol potently protected against LPS-induced ALI and the protective effects of magnolol may attribute partly to the suppression of COX-2 expression.

KEY WORDS

lipopolysaccharide acute lung injury magnolol cyclooxygenase-2 tumor necrosis factor-α interleukin-1β 

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Yun Feng Ni
    • 1
  • Tao Jiang
    • 1
  • Qing Shu Cheng
    • 1
  • Zhong Ping Gu
    • 1
  • Yi Fang Zhu
    • 1
  • Zhi Pei Zhang
    • 1
  • Jian Wang
    • 1
  • Xiao Long Yan
    • 1
  • Wu Ping Wang
    • 1
  • Chang Kang Ke
    • 1
  • Yong Han
    • 1
  • Xiao Fei Li
    • 1
  1. 1.Department of Thoracic Surgery, Tangdu HospitalThe Fourth Military Medical UniversityXi’anChina

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