, Volume 35, Issue 4, pp 1477–1486 | Cite as

Inhibitory Effects of Ginkgo biloba Extract on Inflammatory Mediator Production by Porphyromonas gingivalis Lipopolysaccharide in Murine Macrophages via Nrf-2 Mediated Heme Oxygenase-1 Signaling Pathways

  • Eun Yeon Ryu
  • Ah Jeong Park
  • Sun Young Park
  • Sung Hae Park
  • Hye Won Eom
  • Young Hun Kim
  • Geuntae Park
  • Sang-Joon LeeEmail author


Periodontitis is an oral chronic inflammatory disease that influences systemic diseases. Heme oxygenase-1 has several beneficial abilities through Nrf-2 regulation. Ginkgo biloba has been reported to have anti-inflammatory effects associated with heme oxygenase-1 (HO-1) expression. In this study, we investigated whether the anti-inflammatory effects of G. biloba were involved with Nrf-2-mediated HO-1 expression in Porphyromonas gingivalis LPS-stimulated RAW264.7 macrophage cells. G. biloba was extracted with ethyl acetate (EGB). EGB exhibited anti-inflammatory activities, which suppressed the production of pro-inflammatory mediators, the activation of mitogen-activated protein kinases, and nuclear translocation of transcription factors. EGB also up-regulated the HO-1 expression, and the Nrf-2 level in the nucleus and its transactivity. Furthermore, reduced pro-inflammatory mediator levels by EGB were inverted in the presence of SnPP. The collective results suggest that the anti-inflammatory effects of EGB are due to the HO-1 expression via up-regulation of Nrf-2 in RAW 264.7 cells stimulated by P. gingivalis LPS.


Ginkgo biloba extract heme oxygenase (HO)-1 inflammation NF-E2 related factor (Nrf)-2 Porphyromonas gingivalis lipopolysaccharide 


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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Eun Yeon Ryu
    • 1
  • Ah Jeong Park
    • 2
  • Sun Young Park
    • 1
  • Sung Hae Park
    • 2
  • Hye Won Eom
    • 2
  • Young Hun Kim
    • 1
  • Geuntae Park
    • 3
  • Sang-Joon Lee
    • 2
    Email author
  1. 1.BIO-IT Fusion Technology Research InstitutePusan National UniversityBusanSouth Korea
  2. 2.Department of MicrobiologyPusan National UniversityBusanSouth Korea
  3. 3.Institute for Research & Industry CooperationPusan National UniversityBusanSouth Korea

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