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Inflammation

, Volume 35, Issue 4, pp 1308–1313 | Cite as

Downregulation of miR-27a* and miR-532-5p and Upregulation of miR-146a and miR-155 in LPS-induced RAW264.7 Macrophage Cells

  • Ying Cheng
  • Wenhua Kuang
  • Yongchang Hao
  • Donglin Zhang
  • Ming Lei
  • Li Du
  • Hanwei Jiao
  • Xiaoru Zhang
  • Fengyang Wang
Article

Abstract

MicroRNAs (miRNAs) are short non-coding RNAs that are involved in the epigenetic regulation of cellular processes. To identify more miRNAs which are involved in the macrophage inflammatory response to lipopolysaccharide (LPS) stimulation and dissect the mechanisms more clearly, microRNA profiling of LPS-treated RAW264.7 macrophage cells was performed by initial high-throughput array-based screen and further real-time RT-PCR validation; bioinformatics approaches were used to analyze the target genes of the differentially expressed miRNAs. Compared to the untreated control, two microRNAs (miR-146a and miR-155) with more than twofold higher expression and two microRNAs (miR-27a* and miR-532-5p) with twofold lower expression were detected by array-based screen, which can be validated by qRT-PCR, and more than 1,000 candidate target genes were detected by at least of one of four different algorithms (TargetScan, PicTar, miRDB, and microRNA.org); with gene ontology classification, we were able to correlate the upregulation and downregulation of miRNA to the differential expression of inflammation-related candidate target gene during LPS-induced inflammation. Our findings may provide the basic information for the precise roles of miRNAs in the macrophage inflammatory response to LPS stimulation in the future.

KEY WORDS

LPS RAW264.7 microarray microRNAs target gene 

Notes

ACKNOWLEDGMENTS

This study was financially supported by the Major National Science and Technology Specific Projects (no. 2009ZX08007-009B) and the National High Technology Research and Development Program of China (2011AA100302).

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Ying Cheng
    • 1
  • Wenhua Kuang
    • 1
  • Yongchang Hao
    • 1
  • Donglin Zhang
    • 1
  • Ming Lei
    • 1
  • Li Du
    • 1
  • Hanwei Jiao
    • 1
  • Xiaoru Zhang
    • 1
  • Fengyang Wang
    • 1
  1. 1.College of Agriculture, Hainan Key Lab of Tropical Animal Reproduction & Breeding and Epidemic Disease Research (Construction Period), Animal Genetic Engineering Key Lab of HaikouHainan UniversityHaikouPeople’s Republic of China

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