Inflammation

, Volume 35, Issue 3, pp 1119–1131 | Cite as

A Distinct Regulatory Role of Th17 Cytokines IL-17A and IL-17F in Chemokine Secretion from Lung Microvascular Endothelial Cells

  • Hitomi Fujie
  • Kaijun Niu
  • Michiru Ohba
  • Yoshihisa Tomioka
  • Haruki Kitazawa
  • Kengo Nagashima
  • Takashi Ohrui
  • Muneo Numasaki
Article

Abstract

Th17 cytokines IL-17A and IL-17F play a critical role in the activation and recruitment of neutrophils at airway inflammation mainly through the induction of CXC chemokines in the lungs. Vascular endothelial cells belong to the category of major CXC chemokine-producing cells. However, until now, the precise role of Th17 cytokines in CXC chemokine secretion in lung microvascular endothelial cells (LMVECs) has not been fully elucidated. In this study, we examined the biological effects of Th17 cytokines IL-17A and IL-17F on CXCL1, CXCL5, and CXCL8 release in LMVECs. Both IL-17 receptor A (IL-17RA) and IL-17RC are expressed on the surface of LMVECs. In contrast to IL-17F, IL-17A significantly upregulated CXCL1 mRNA expression and protein release, whereas both IL-17A and IL-17F did not have the ability to induce CXCL5 and CXCL8 secretion in LMVECs. IL-17A and IL-17F displayed positive regulatory effects on IL-1β-induced CXCL1, CXCL5, and CXCL8 secretion. On the other hand, IL-17A enhanced the upregulating effect of TNF-α on CXCL1, CXCL5, and CXCL8 release, whereas IL-17F had a negative regulatory effect on TNF-α-mediated secretion. Th2 cytokines IL-4 and IL-13 showed an inhibitory effect on IL-1β plus IL-17A-induced CXCL1, CXCL5, and CXCL8 secretion, but displayed a positive regulatory effect on TNF-α plus IL-17A-induced secretion. These results provide evidence that Th17 cytokines IL-17A and IL-17F have a distinct regulatory role in CXCL1, CXCL5, and CXCL8 expression in LMVECs stimulated either with IL-1β or with TNF-α. Our findings also suggest that CXC chemokine secretion in LMVECs may be complicatedly regulated by Th17 cytokines, Th2 cytokines, and macrophage-associated cytokines in pathological conditions such as bronchial asthma.

KEY WORDS

Th17 vascular endothelial cell chemokine 

ABBREVIATIONS

IL

Interleukin

TNF

Tumor necrosis factor

mAb

Monoclonal antibody

hr

Hour

mRNA

Messenger ribonucleic acid

GRO

Growth-related oncogene

GCP

Granulocyte chemotactic protein

ENA

Epithelial cell-derived neutrophil-activating protein

MCP-1

Monocyte chemotactic protein-1

S.D.

Standard deviation

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Hitomi Fujie
    • 1
  • Kaijun Niu
    • 2
  • Michiru Ohba
    • 1
  • Yoshihisa Tomioka
    • 3
  • Haruki Kitazawa
    • 4
  • Kengo Nagashima
    • 5
  • Takashi Ohrui
    • 6
  • Muneo Numasaki
    • 1
  1. 1.Department of Nutrition Physiology, Faculty of Pharmaceutical SciencesJosai UniversitySakadoJapan
  2. 2.Laboratory of Health & Sports Science, Division of Biomedical Engineering for Health & WelfareTohoku University Graduate School of Biomedical EngineeringSendaiJapan
  3. 3.Laboratory of Oncology, Pharmacy Practice and Sciences, Graduate School of Pharmaceutical SciencesTohoku UniversitySendaiJapan
  4. 4.Food Immunology Group, Laboratory of Animal Products Chemistry, Graduate School of Agricultural ScienceTohoku UniversitySendaiJapan
  5. 5.Laboratory of Biostatistics, Faculty of Pharmaceutical SciencesJosai UniversitySakadoJapan
  6. 6.Division of Geriatric Pharmacology, Institute of Development, Aging and CancerTohoku UniversitySendaiJapan

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