Inflammation

, Volume 35, Issue 3, pp 1023–1030 | Cite as

Fractalkine Upregulates Inflammation through CX3CR1 and the Jak–Stat Pathway in Severe Acute Pancreatitis Rat Model

  • Li-ya Huang
  • Ping Chen
  • Ling-xiao Xu
  • Yu-fen Zhou
  • Yong-ping Zhang
  • Yao-zong Yuan
Article

Abstract

Based on the function of chemokine fractalkine (FKN), acting as both adhesion and chemoattractant, FKN plays a role in acute inflammatory response. In this study, we investigated the mechanism of FKN mediated upregulation inflammation in severe acute pancreatitis (SAP) rat models. Western blot, reverse transcriptase-polymerase chain reaction, and immunofluorescence demonstrated that FKN and its receptor CX3CR1 were overexpressed in cerulein-stimulated AR42J cells. AG490 and FKN-siRNA inhibited activation of Janus kinase/signal transducers and activators of transcription (Jak/Stat) in cerulein-stimulated AR42J cells. Following exposure AG490 and FKN-siRNA inhibited tumor necrosis factor-alpha expression by enzyme-linked immunosorbent assay and immunohistochemistry in vivo the SAP rat models. These results showed FKN and CX3CR1 were involved inflammatory response in cerulein-stimulated AR42J cells. FKN upregulates inflammation through CX3CR1 and the Jak/Stat pathway in SAP rat models.

KEY WORDS

chemokine fractalkine CX3CR1 severe acute pancreatitis Jak/Stat 

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Li-ya Huang
    • 2
  • Ping Chen
    • 1
  • Ling-xiao Xu
    • 1
  • Yu-fen Zhou
    • 1
  • Yong-ping Zhang
    • 1
  • Yao-zong Yuan
    • 1
  1. 1.Department of GastroenterologyRuijin Hospital Shanghai Jiaotong University School of MedicineShanghaiChina
  2. 2.Department of GastroenterologyAffiliated Hospital of Ningxia Medical UniversityYinchuanChina

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