Beneficial Effect of Interleukin-1 Receptor Antagonist Protein on Spinal Cord Injury Recovery in the Rat
- 303 Downloads
We assessed the effect of treatment with the interleukin-1 receptor antagonist protein (IRAP) on morphological and functional recovery in a rat model of SCI. All sections were processed for immunohistochemistry, hematoxylin–eosin, and Nissl staining. Rats were assessed for hind limb motor function using the Basso, Beattie, and Bresnahan (BBB) hind limb locomotor rating scale and the inclined plane test. At 1, 48, and 72 h after operation, there was a significant increase in neurofilament proteins and brain-derived neurotrophic factor expression in the IRAP group I when compared with the saline group I and the sham-operated group I (P < 0.05). The mean inclined plane scores and BBB scores for the IRAP group II were higher than the saline group II at 1, 2, 3, and 4 weeks post-injury (P < 0.05). In conclusion, treatment with IRAP enhanced neuronal survival after SCI.
KEY WORDSinterleukin-1 receptor antagonist protein spinal cord injury rat inflammation behavior
This study was supported by Dr. Start Fund of Guangxi Medical University (308010) and Research Foundation of Guangxi Education Department (Gui Education200710LX063).
Conflict of Interest Statement
The authors declare that there are no conflicts of interest.
- 13.Maharajh, G.S., E.A. Pascoe, W.C. Halliday, H.P. Grocott, D.B. Thiessen, L.G. Girling, M.S. Cheang, and W.A. Mutch. 1996. Neurological outcome in a porcine model of descending thoracic aortic surgery. Left atrial-femoral artery bypass versus clamp/repair. Stroke 27: 2095–2100.PubMedCrossRefGoogle Scholar
- 19.Rapalino, O., O. Lazarov-Spiegler, E. Agranov, G.J. Velan, E. Yoles, M. Fraidakis, A. Solomon, R. Gepstein, A. Katz, M. Belkin, M. Hadani, and M. Schwartz. 1998. Implantation of stimulated homologous macrophages results in partial recovery of paraplegic rats. Nat Med 4: 814–821.PubMedCrossRefGoogle Scholar
- 21.Sasaki, M., C. Radtke, A.M. Tan, P. Zhao, H. Hamada, K. Houkin, O. Honmou, and J.D. Kocsis. 2009. BDNF-hypersecreting human mesenchymal stem cells promote functional recovery, axonal sprouting, and protection of corticospinal neurons after spinal cord injury. The Journal of Neuroscience 29: 14932–14941.PubMedCrossRefGoogle Scholar