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Inflammation

, Volume 35, Issue 2, pp 409–419 | Cite as

Dynamic Mobility of Immunological Cells Expressing S100A8 and S100A9 in vivo: A Variety of Functional Roles of the two Proteins as Regulators in Acute Inflammatory Reaction

  • Akiko KoikeEmail author
  • Satoshi Arai
  • Sachiko Yamada
  • Akiko Nagae
  • Naoko Saita
  • Hiroshi Itoh
  • Shinji Uemoto
  • Masayuki Totani
  • Masaki Ikemoto
Article

Abstract

The immunological properties of rat S100A8 (r-S100A8) and S100A9 (r-S100A9) in immune cells are poorly understood. Enzyme-linked immunosorbent assay (ELISA) for r-S100A9 enabled us to discuss the differential functional roles of the two proteins, and their localization in the cells was observed microscopically. Recombinant human S100A8 (rh-S100A8) or S100A9 (rh-S100A9) were intravenously administrated into rats with LPS-induced liver damage. ELISA was used to measure the serum concentration of S100A9 in the rats. Western blotting and a preparative ELISA were used to prove specificity and avidity of monoclonal antibodies for r-S100A8 and r-S100A9. Immunohistochemical staining was carried out to visualize intracellular localization of the two proteins in the immune cells using the antibodies. When rh-S100A8 was intravenously injected in the rats (B group), the serum concentration of r-S100A9 apparently decreased as compared with that of the positive control rats (A group). The activities of AST, ALT, and LD in the rat sera (B group) also significantly went down in comparison with those of the rats (A group). Although both the S100A8 and S100A9 were abundantly expressed in activated immune cells, quite difference of not only their intracellular localization but also distribution of the cells expressing the two proteins was microscopically observed. In the rats (B group), less number of the immune cells or less amount of r-S100A8 and r-S100A9 in the cells than those of the rats (A group) was also seen. The r-S100A8 could serve as a regulator of acute inflammatory reaction in the rats with LPS-induced damage.

KEY WORDS

S100A8 S100A9 acute inflammation neutrophils macrophages lipopolysaccharide 

ABBREVIATIONS

Rat S100A8

r-S100A8

Rat S100A9

r-S100A9

Recombinant human S100A8

rh-S100A8

Recombinant human S100A9

rh-S100A9

LPS

Lipopolysaccharide

STA

Streptavidin

HRP

Horseradish peroxidase

FITC

Fluorescein 5-isothiocyanate

SDS-PAGE

Polyacrylamide gel electrophoresis in the presence of SDS

Notes

ACKNOWLEDGEMENTS

We thank Dr Hiroshi Murayama (Yamasa Shoyu Co., Choshi, Chiba, Japan) for the gift of ELISA plates, and Dr Kyoichi Matsumoto (Mikuri Immunological Laboratory Co., Osaka, Japan) for his technical teaching in the preparation of monoclonal antibody. This work was supported by a Grant-in-Aid for Scientific Research (C: 20590567) from the Ministry of Education, Science, Sports and Culture of Japan (to M.I.)

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Akiko Koike
    • 1
    Email author
  • Satoshi Arai
    • 2
  • Sachiko Yamada
    • 1
  • Akiko Nagae
    • 1
  • Naoko Saita
    • 1
  • Hiroshi Itoh
    • 1
  • Shinji Uemoto
    • 3
  • Masayuki Totani
    • 4
  • Masaki Ikemoto
    • 1
  1. 1.Human Health Sciences, Graduate School of MedicineKyoto UniversitySakyo-kuJapan
  2. 2.Immunological Laboratory, Diagnostic Division, Yamasa Shoyu Co., LtdChoshiJapan
  3. 3.Department of Transplantation UnitKyoto University HospitalKyotoJapan
  4. 4.Graduate School of Human Life ScienceShowa Women’s UniversityTokyoJapan

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