Possible Protective Role of Chloramphenicol in TSST-1 and Coagulase-Positive Staphylococcus aureus-Induced Septic Arthritis with Altered Levels of Inflammatory Mediators
Chloramphenicol is mostly used against coagulase-negative Staphylococcus aureus, and its protective role against coagulase-positive S. aureus is not well studied. In our study, arthritis was induced in mice by S. aureus (Apollo Gleneagles 33 (AG-33) or American Type Culture Collection 25923 (ATCC-25923)) infection. Chloramphenicol was administered after 2 h of infection. Mice were killed at 1, 3, 5 days post-infection. Mice inoculated with pathogenic Staphylococci (AG-33) expressing coagulase and Toxic shock syndrome toxin-1 (TSST-1), displayed severe arthritis with enhanced bacterial burden in the spleen, cytokine production in serum and synovial tissue, neutrophil recruitment, and cyclooxegenase-2 expression in synovial tissue compared with ATCC-25923-infected groups. Severity of arthritis was regulated by chloramphenicol treatment. Our study suggests that alteration in the inflammatory cytokine levels and pronounced production of cyclooxygenase-2 play important roles in progression of arthritis which is regulated by application of chloramphenicol.
KEY WORDSbacterial arthritis chloramphenicol inflammatory cytokine Staphylococcus aureus toxic shock syndrome toxin-1
The author (Biswadev Bishayi) thanks the University of Calcutta for providing the fellowship to Ms. Sayantani Majumdar (Sanction No UGC/1000/Fellow (Univ) date 13 Oct 2009) under the scheme of University Research Fellowship (URF), University of Calcutta from University Grants Commission, New Delhi, India. The corresponding author also thanks Professor Dhrubojyoti Chattopadhyay, Department of Biochemistry, University of Calcutta for providing facilities for PCR.
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