Inflammation

, Volume 31, Issue 2, pp 91–98

The Treatment with Antibody of TNF-α Reduces the Inflammation, Necrosis and Fibrosis in the Non-alcoholic Steatohepatitis Induced by Methionine- and Choline-deficient Diet

  • Suleyman Serdar Koca
  • Ibrahim Halil Bahcecioglu
  • Orhan Kursat Poyrazoglu
  • Ibrahim Hanifi Ozercan
  • Kazim Sahin
  • Bilal Ustundag
Article

Abstract

To assess the effects of anti-TNF-α antibody (infliximab) in experimental steatohepatitis induced by methionine- and choline-deficient (MCD) diet. The study included thirty rats. One group received normal rat food, and two groups received MCD diet. The treatment group received a single dose intra-peritoneal infliximab (4 mg/kg), at week 8. MCD diet increased levels of AST, ALT, TNF-α, TGF-β1, tissue and plasma MDA (p < 0.05 for each). Moreover, it led to steatosis, ballooning degeneration, inflammation, fibrosis and increased actin expression, histopathologically (p < 0.05 for each). In this experimental steatohepatitis anti-TNF-α antibody decreased the levels of AST, ALT, TGF-β1 and plasma and tissue MDA (p < 0.05 for each). Moreover, inflammation, necrosis, actin expression and fibrosis decreased in anti-TNF-α group compared to placebo group (p < 0.05 for each). This study indicates that anti-TNF-α antibody is effective on necrosis, inflammation and fibrosis in the experimental model of non-alcoholic steatohepatitis, induced by MCD diet.

Key words

steatohepatitis methionine- and choline-deficient diet infliximab 

Copyright information

© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  • Suleyman Serdar Koca
    • 1
    • 6
  • Ibrahim Halil Bahcecioglu
    • 2
  • Orhan Kursat Poyrazoglu
    • 2
  • Ibrahim Hanifi Ozercan
    • 3
  • Kazim Sahin
    • 4
  • Bilal Ustundag
    • 5
  1. 1.Faculty of Medicine, Department of Internal Medicine and RheumatologyFırat UniversityElazigTurkey
  2. 2.Faculty of Medicine, Department of GastroenterologyFırat UniversityElazigTurkey
  3. 3.Faculty of Medicine, Department of PathologyFırat UniversityElazigTurkey
  4. 4.Faculty of Veterinary, Department of Animal NutritionFırat UniversityElazigTurkey
  5. 5.Faculty of Medicine, Department of BiochemistryFırat UniversityElazigTurkey
  6. 6.Tıp FakultesiFırat universitesiElazigTurkey

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