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Inflammation

, Volume 30, Issue 6, pp 213–223 | Cite as

Inner Anti-inflammatory Mechanisms of Petroleum Ether Extract from Melilotus suaveolens Ledeb

  • Lei Zhao
  • Jun-Yan Tao
  • Shu-Ling Zhang
  • Ran Pang
  • Feng Jin
  • Ji-Hua Dong
  • Yuan-Jin Guo
Article

Abstract

Summary

Melilotus suaveolens Ledeb is a species of traditional medical plant for treating inflammation-related disease. This study is to explore the inner anti-inflammatory mechanism on petroleum ether extract from Melilotus suaveolens Ledeb.

Materials and Methods

Inflammatory cellular model was founded by intervention of lipopolysaccharide (LPS) on RAW264.7 cell line. Secretion of TNF-α, IL-1β, IL-6, NO and IL-10 in supernatant, mRNA expression of TNF-α, COX-2, iNOS and HO-1, protein expression of COX-2 and HO-1, activation of NF-κB and ingredients in the extract were assayed.

Results

The extract could not only reduce production of pro-inflammatory mediators by blocking NF-κB activation but promote release of anti-inflammatory mediator HO-1 significantly. The only active ingredient in the extract was coumarin and the concentration of coumarin in each 1 g extract was 0.27822 mg.

Conclusion

Compared to Dexamethasone, the extract not only has similar effects on antagonizing pro-inflammatory mediators and cytokines but has effects on promoting production of anti-inflammatory mediators.

Key words

Melilotus suaveolens Ledeb petroleum ether extract RAW 264.7 cell line NF-kappaB HO-1 

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Copyright information

© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  • Lei Zhao
    • 1
  • Jun-Yan Tao
    • 2
  • Shu-Ling Zhang
    • 1
  • Ran Pang
    • 1
  • Feng Jin
    • 3
  • Ji-Hua Dong
    • 4
  • Yuan-Jin Guo
    • 5
  1. 1.Department of Hepatology and Infectious Disease, Union Hospital, Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanPeople’s Republic of China
  2. 2.College of Life Science and TechnologyHuazhong University of Science and TechnologyWuhanPeople’s Republic of China
  3. 3.Department of Neurosurgery, Union Hospital, Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanPeople’s Republic of China
  4. 4.Central Lab, Union Hospital, Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanPeople’s Republic of China
  5. 5.Department of Neurology, Union Hospital, Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanPeople’s Republic of China

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