, 30:52 | Cite as

Ozonotherapy in an Induced Septic Shock. I. Effect of Ozonotherapy on Rat Organs in Evaluation of Free Radical Reactions and Selected Enzymatic Systems

  • Pawel Madej
  • Andrzej Plewka
  • Janusz A. Madej
  • Marcin Nowak
  • Danuta Plewka
  • Grzegorz Franik
  • Darek Golka


The confirmed advantageous effects of oxygen/ozone therapy in several clinical conditions stimulated experimental studies on effects of the therapy in rats with an induced septic shock. The studies were conducted on adult male rats of Wistar strain. Four groups of the animals, each of 15 rats, included: I—control group, (C); II—animals intraperitoneally administered with O2/O3 (CO), III—rats given of Escherichia coli endotoxin (lipopolysaccharide—LPS) (CL), IV—rats administered with the lipopolysaccharide plus administered with the oxygen/ozone mixture (OL). Activities of catalase and superoxide dismutase and of free radical reactions were estimated. The exposure to LPS augmented activities of SOD and of catalase in liver, lungs and heart. In all the examined organs LPS induced significant changes in levels of free radicals. Except of the lungs, parallel administration of the rats with LPS and ozone/oxygen revoked development of the alterations. The obtained results point to a strong, stabilizing and regenerative effect of ozonotherapy.

Key words

septic shock ozonotherapy oxidative stress free radicals rats 


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Copyright information

© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  • Pawel Madej
    • 1
  • Andrzej Plewka
    • 2
  • Janusz A. Madej
    • 3
  • Marcin Nowak
    • 3
  • Danuta Plewka
    • 4
  • Grzegorz Franik
    • 1
  • Darek Golka
    • 5
  1. 1.Department and Clinic of Gynaecological EndocrinologyMedical University of SilesiaKatowicePoland
  2. 2.Department of Protein Chemistry and EnzymologyMedical University of SilesiaSosnowiecPoland
  3. 3.Department of Pathological Anatomy and Forensic MedicineVeterinary AcademyWroclawPoland
  4. 4.Department of Histology and EmbryologyMedical University of SilesiaKatowicePoland
  5. 5.Department of PathomorphologyMedical University of SilesiaKatowicePoland

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