, Volume 30, Issue 1–2, pp 44–51 | Cite as

Hydrogen Peroxide in Exhaled Breath Condensate (EBC) vs Eosinophil Count in Induced Sputum (IS) in Parenchymal vs Airways Lung Diseases"

  • Elizabeth Fireman
  • Moshe Shtark
  • Israel E. Priel
  • Robert Shiner
  • Ram Mor
  • Shmuel Kivity
  • Zvi Fireman


We compared exhaled breath condensate (EBC) and induced sputum (IS) for assessing inflammation in pulmonary diseases in patients with obstructive lung disease (n = 20), persistent cough >6 months (n = 20), interstitial lung disease (n = 25) and controls (n = 10). EBC was collected by suspending a Teflon perfluoroalkoxy tube installed in an ice-filled container and connected to a polypropylene test tube. IS was recovered after 20’ inhalation of 3% saline with an ultrasonic nebulizer, and 300 cells were differentially counted in cytospin Giemsa-stained slides. H202 was measured by a method based on oxidation of phenolsulfonphthalein (phenol red) mediated by horseradish peroxidases and H202. Pulmonary function tests were performed by conventional methods. H202 levels in EBC and % eosinophils in IS were significantly different between groups. A positive and significant correlation was found between % eosinophils in IS and the levels of H202 in EBC for each group and for all patients combined.

Key words

exhaled breath condensate induced sputum chronic inflammatory lung diseases biomarker 



Esther Eshkol is thanked for editorial assistance.


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Copyright information

© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  • Elizabeth Fireman
    • 1
    • 7
  • Moshe Shtark
    • 1
  • Israel E. Priel
    • 2
    • 3
  • Robert Shiner
    • 4
  • Ram Mor
    • 1
  • Shmuel Kivity
    • 1
  • Zvi Fireman
    • 5
    • 6
  1. 1.Institute of Pulmonary and Allergic DiseasesTel-Aviv Sourasky Medical Center and National Laboratory Service for ILD, Sakler Faculty of Medicine, Tel-Aviv UniversityTel-AvivIsrael
  2. 2.The Department of Pulmonary Medicine, Sackler Faculty of MedicineTel-Aviv UniversityTel-AvivIsrael
  3. 3.The Edith Wolfson Medical Center, Sackler Faculty of MedicineTel-Aviv UniversityTel-AvivIsrael
  4. 4.NHLIImperial College School of MedicineLondonUK
  5. 5.Gastroenterology DepartmentHillel Yaffe Medical CenterHaderaIsrael
  6. 6.Rappapot Faculty of MedicineTechnionHaifaIsrael
  7. 7.Institute of Pulmonary and Allergic DiseasesTel-Aviv Sourasky Medical Center and Laboratory Service for ILDTel-AvivIsrael

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