Hydrogen Peroxide in Exhaled Breath Condensate (EBC) vs Eosinophil Count in Induced Sputum (IS) in Parenchymal vs Airways Lung Diseases"
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We compared exhaled breath condensate (EBC) and induced sputum (IS) for assessing inflammation in pulmonary diseases in patients with obstructive lung disease (n = 20), persistent cough >6 months (n = 20), interstitial lung disease (n = 25) and controls (n = 10). EBC was collected by suspending a Teflon perfluoroalkoxy tube installed in an ice-filled container and connected to a polypropylene test tube. IS was recovered after 20’ inhalation of 3% saline with an ultrasonic nebulizer, and 300 cells were differentially counted in cytospin Giemsa-stained slides. H202 was measured by a method based on oxidation of phenolsulfonphthalein (phenol red) mediated by horseradish peroxidases and H202. Pulmonary function tests were performed by conventional methods. H202 levels in EBC and % eosinophils in IS were significantly different between groups. A positive and significant correlation was found between % eosinophils in IS and the levels of H202 in EBC for each group and for all patients combined.
Key wordsexhaled breath condensate induced sputum chronic inflammatory lung diseases biomarker
Esther Eshkol is thanked for editorial assistance.
- 4.Pizzichini, E., M. M. Pizzichini, A. Efthimiadis, S. Evans, M. M. Morris, D. Squillace, G. J. Gleich, J. Dolovich, F. E. Hargreave. 1996. Indices of airway inflammation in induced sputum: reproducibility and validity of cell and fluid-phase measurements. Am. J. Respir. Crit. Care Med. 154:308–317.PubMedGoogle Scholar
- 5.Fahy, J. V., H. A. Boushey, S. C. Lazarus, E. A. Mauger, R. M. Cherniack, V. M. Chinchilli, T. J. Craig, J. M. Drazen, J. G. Ford, J. E. Fish, E. Israel, M. Kraft, R. F. Lemanske, R. J. Martin, D. McLean, S. P. Peters, C. Sorkness, S. J. Szefler. NHLBI Asthma Clinical Research Network. 2001. Safety and reproducibility of sputum induction in asthmatic subjects in a multicenter study. Am. J. Respir. Crit. Care Med. 163:1470–1475.PubMedGoogle Scholar
- 6.Horvath, I., J. Hunt, P. J. Barnes, K. Alving, A. Antczak, E. Baraldi, G. Becher, W. J. van Beurden, M. Corradi, R. Dekhuijzen, R. A. Dweik, T. Dwyer, R. Effros, S. Erzurum, B. Gaston, C. Gessner, A. Greening, L. P. Ho, J. Hohlfeld, Q. Jobsis, D. Laskowski, S. Loukides, D. Marlin, P. Montuschi, A. C. Olin, A. E. Redington, P. Reinhold, E. L. van Rensen, I. Rubinstein, P. Silkoff, K. Toren, G. Vass, C. Vogelberg, H. Wirtz, and ATS/ERS Task Force on Exhaled Breath Condensate. 2005. Exhaled breath condensate: methodological recommendations and unresolved questions. Eur. Respir. J. 26:371–372.CrossRefGoogle Scholar
- 10.National Heart, Lung, and Blood Institute. 1995. Global strategy for asthma management and prevention. In: Global initiative for asthma. National Institutes of Health. Bethesda, MD: Publication No. 95–3659.Google Scholar
- 11.Pauwels, R. A., A. S. Buist, P. M. Calverley, C. R. Jenkins, S. S. Hurd, and GOLD Scientific Committee. 2001. Global strategy for the diagnosis, management, and prevention of chronic obstructive pulmonary disease. NHLBI/WHO Global Initiative for Chronic Obstructive Lung Disease (GOLD) Workshop summary. Am. J. Respir. Crit. Care Med. 163:1256–1276.PubMedGoogle Scholar
- 12.American Thoracic Society (statement). 1987. Standardization of spirometry–1987. Update. Am. Rev. Respir. Dis. 136:1285–1298Google Scholar
- 24.Fireman, E., B. Boikaner, and I. Priel. 2006. Combined CD4/CD8 ratio in induced sputum and pulmonary function testing for non-invasive identification of sarcoidosis. Transl Research (Past J. Lab. Clin. Med.) 148(2):87–95.Google Scholar
- 28.Kharitonov, S. A., and P. J. Barnes. 2003. Exhaled nitric oxide, carbon monoxide, and breath condensate. In: M. Nandor (ed). Lung Biology in Health and Disease Markers in Exhaled Breath vol 170. Portland, Marcel Dekker, pp 199–218.Google Scholar
- 30.Horvath, I., L .E. Donnelly, A. Kiss, P. Paredi, S. A. Kharitonov, and P. J. Barnes. 1998. Raised levels of exhaled carbon monoxide are associated with an increased expression of heme oxygenase-1 in airway macrophages in asthma: a new marker of oxidative stress. Thorax 53:668–672.PubMedCrossRefGoogle Scholar