Mossbauer study of different factors influence on donated blood quality
- 54 Downloads
Packed red blood cells (RBC) are main blood components used in transfusiology. During storage, preserved blood cells undergo progressive structural and functional changes that may reduce red-cell function and viability after transfusion. As the hemoglobin is the main and active component of erythrocytes, we consider that oxidized hemoglobin derivatives formation cause those changes. With the aim of developing the new criteria for donated blood suitability for transfusion, we studied storage-related changes in red blood cells hemoglobin by means of analytical fitting of Mössbauer spectra. We analyzed Mössbauer spectra of set RBC’s samples from volunteer donors every few days during their storage. Using those data, we built kinetic curves of various hemoglobin derivatives changes during RBC storage for different hemoglobin forms concentration in RBC samples. We found that kinetic curves are specific for each donor. They also depend on the donor’s age and health condition at the time of donation. Based on our spectroscopy results we suggested an objective criteria for duration of storage of regular donors RBC.
KeywordsMössbauer spectroscopy Blood donation Red blood cells Hemoglobin derivatives
The authors are grateful to donor volonteers, Moscow Government Departament of Health, Gematology scintific center of Russian Academy of Science and Moscow State University Program of Development.
- 1.Russian State Standard No. 52938 2008: Donated Blood and Its Components, March 1, 2009Google Scholar
- 2.Basran, S., Frumento, R.J., Cohen, A., et al: The association between duration of storage of transfused red blood cells and morbidity and mortality after reoperative cardiac surgery. Anesth Analg 103(1), 15–20 (2006). https://doi.org/10.1213/01.ane.0000221167.58135.3d CrossRefGoogle Scholar
- 3.Koch, C.G., Li, L., Sessler, D.S., Figueroa, P., et al: Duration of red-cell storage and complications after cardiac surgery. England J. Med. 358, 1229–39 (2008).Google Scholar
- 4.Klenova, N.A.: Biochemical mechanisms of erythrocytes disintegration in different operating conditions Extended Abstract of Doctoral Sci. (Biol.) Dissertation, Samara State Univ. (2003)Google Scholar
- 5.Moshkovskii, Yu.Sh.: Mössbauer spectroscopy in biology. In: Goldanskii, V.I. (ed.) Chemical Applications of Mössbauer Spectroscopy, pp 414–437. Mir, Moscow (1970)Google Scholar
- 6.Maling, J.E., Weisbluth, M.: The application of Mossbauer spectroscopy to the Study of Iron in Heme Protein. In: Wyard, S.J. (ed.) Solid State Physics in Biology and Medicine. Ch.10. McGrandHill&Co (1968)Google Scholar
- 8.Semin, B.K, Novakova, A.A, Aleksandrov, A.Yu, Ivanov, I.I, Rubin, A.B, Kuzmin, R.N.: Mossbauer spectroscopy of iron metabolism and iron intercellular distribution in liver of rat. Biochimica et Biophysica Acta - General Subjects. 715(1), 52–56 (1982). https://doi.org/10.1016/0304-4165(82)90048-4 CrossRefGoogle Scholar
- 13.Novakova, A.A., Kiseleva, T.Y.u., Potapkin, V.B., et al.: Mossbauer diagnostics of intravenous laser irradiation influence on hemoglobin state in human erythrocytes. Almanac J. Clin. Med. 17–2, 119–122 (2008)Google Scholar
- 14.Khvastunov, S.M., Novakova, A.A., Kiseleva, T.Y.u.: Mössbauer diagnostics of hemoglobin in red blood cells during its storage. J. Med. Phys. (Russian) 52(4), 38–42 (2011)Google Scholar
- 15.Kiseleva, T.Y.u., Novakova, A.A., Khvastunov, S.M.: Mössbauer study of the individual donor factors influence on the donor blood state during its storage. In: Proceedings of the 5th Troitsk Conf.“Medical Physics and Innovat. in Medicine” (in Russian), vol. 2, 127. Moscow (2012)Google Scholar
- 16.Khvastunov, S.M., Novakova, A.A., Kiseleva, T.Y.u.: Application of Mössbauer spectroscopy to study of donor’s blood hemoglobin. Izv. Ran. Ser. Phys. 77(6), 804–807 (2013)Google Scholar