Hyperfine Interactions

, 239:6 | Cite as

Investigation of biogenic iron-containing nanoscale composite materials

  • Z. Cherkezova-Zheleva
  • D. Paneva
  • M. Shopska
  • R. Ilieva
  • M. Iliev
  • D. Kovacheva
  • G. Kadinov
Part of the following topical collections:
  1. Proceedings of the International Conference on the Applications of the Mössbauer Effect (ICAME 2017), Saint-Petersburg, Russia, 3-8 September 2017


Biogenic composites are interesting as green and sustainable precursors for preparation of advanced materials of various applications. Biomaterials from Leptothrix group bacteria cultivated in two feeding media of silicon-iron-glucose-peptone (SIGP) and Lieske were studied. Iron-containing biogenic powders and biofilms on silica gel covered aluminum plates were prepared. They were studied to elucidate the effect of the plates in the process of growing biogenic iron nanotubes. The cultivation period was varied from 4 to 30 days. Biomass phase composition and physicochemical properties were studied by Mössbauer spectroscopy at room and low temperature, as well as by means of powder XRD, FTIRS, and SEM methods. Mössbauer analysis offered us a unique possibility to register iron oxide species at the different steps of biogenic material formation. Tetrahedrally coordinated iron species were registered at an early stage of biofilm formation. So, important results on the mechanism of biomineralization process are obtained. The reaction of CO oxidation on prepared biomaterials was studied using in situ DRIFTS. Comparative analysis of the obtained materials and examination of spent samples showed differences in their phase composition, stability and dispersity. Changes of the phase composition were observed during catalytic tests with biomasses obtained in Lieske feeding media. No differences in biogenic powder and biofilm composition and dispersity were registered when SIGP media was used neither for studied bacteria cultivation period nor in spent catalysts. These results indicated the stabilizing effect of extra silicon content in the feeding media. Formation of iron oxihydroxide nanotubes was also established in this case.


Biogenic nanosized iron oxides Leptothrix genus of bacteria Mössbauer spectroscopy X-ray diffraction IR spectroscopy 



The authors are grateful to the Bulgarian Science Fund for financial support through project T02-17/2014. The authors thank Assoc. Prof. K. Starbova and Assoc. Prof. N. Starbov from the Institute of Solid State Physics, Bulgarian Academy of Sciences for SEM visualization.


  1. 1.
    Barsani, L., Gualteri, P., Evangelista, V. (eds.): Molecular Electronics: Bio-Sensors and Bio-computers (NATO Science Series, II. Mathematics, Physics and Chemistry, Vol. 96),. Kluwer Acad. Publ., Norwell (2002)Google Scholar
  2. 2.
    Hennebel, T., Gusseme, B., Boon, N., Verstraete, W.: Biogenic metals in advanced water treatment. Trends Biotechnol. 27, 90–98 (2008)CrossRefGoogle Scholar
  3. 3.
    Gupta, A.K., Gupta, M.: Synthesis and surface engineering of iron oxide nanoparticles for biomedical applications. Biomaterials 26, 3995–4021 (2005)CrossRefGoogle Scholar
  4. 4.
    Ellis, D.: Microbiology of the iron-depositing bacteria. Wexford College Press, Palm Springs CA (2003)Google Scholar
  5. 5.
    Kappler, A., Straub, K.L.: Geomicrobiological cycling of iron. Rev. Miner. Geochem. 59, 85–108 (2005)CrossRefGoogle Scholar
  6. 6.
    Sawayama, M., Suzuki, T., Hashimoto, H., Kasai, T., Furutani, M., Miyata, N., Kunoh, H., Takada, J.: Isolation of a Leptothrix strain, OUMS1, from ocherous deposits in groundwater J. Curr. Microbiol. 63, 173– 180 (2011)CrossRefGoogle Scholar
  7. 7.
    Hashimoto, H., Yokoyama, S., Asaoka, H., Kusano, Y., Ikeda, Y., Seno, M., Takada, J., Fujii, T., Nakanishi, M., Murakami, R.: Characteristics of hollow microtubes consisting of amorphous iron oxide nanoparticles produced by iron oxidizing bacteria, Leptothrix ochracea. J. Magnet. Magnet. Mater. 310, 2405–2407 (2007)ADSCrossRefGoogle Scholar
  8. 8.
    Hashimoto, H., Fujii, T., Kohara, S., Nakanishi, K., Yogi, C., Peterlik, H., Nakanishi, M., Takada, J.: Structural transformations of heat-treated bacterial iron oxide. Mater. Chem. Phys. 155, 67–75 (2015)CrossRefGoogle Scholar
  9. 9.
    Wang, X., Zhu, M., Lan, S., Ginder-Vogel, M., Liu, F., Feng, X.: Formation and secondary mineralization of ferrihydrite in the presence. of silicate and Mn (II). Chem. Geol. 415, 37–46 (2015)ADSCrossRefGoogle Scholar
  10. 10.
    Cherkezova-Zheleva Z., Shopska M., Paneva D., Kovacheva D., Kadinov G., Mitov I.: Comparative Study of Biogenic and Abiotic Iron-containing Materials. Hyperfine Interact. vol. 237. paper 56 (2016)Google Scholar
  11. 11.
    Sakai, T., Miyazaki, Y., Murakami, A., Sakamoto, N., Ema, T., Hashimoto, H., Furutani, M., Nakanishi, M., Fujiia, T., Takada, J.: Chemical modification of biogenous iron oxide to create an excellent enzyme scaffold. Org. Biomol. Chem. 8, 336–338 (2010)CrossRefGoogle Scholar
  12. 12.
    Francisco, P.C., Sato, T., Otake, T., Kasama, T.: Kinetics of Fe3+ mineral crystallization from ferrihydrite in the presence of Si at alkaline conditions and implications for nuclear waste disposal. Am. Mineral. 101, 2057–2069 (2016)ADSCrossRefGoogle Scholar
  13. 13.
    Rentz, J.A.: Phosphorous Removal Potential Using Biogenic Iron Oxides. WERF, Alexandria, Co-published by IWA Publishing, London (2010)Google Scholar
  14. 14.
    Pragnesh D., Lakhan C.: J. Nanotechnol. Article ID 398569 (2014)Google Scholar
  15. 15.
    Shopska, M., Paneva, D., Kadinov, G., Todorova, S., Fabian, M., Yordanova, I., Cherkezova-Zheleva, Z., Mitov, I.: Composition and catalytic behaviour in CO oxidation of biogenic iron-containing materials. Reac. Kinet. Mech. Catal. 118, 179–198 (2016)CrossRefGoogle Scholar
  16. 16.
    Shopska, M., Paneva, D., Kadinov, G., Cherkezova-Zheleva, Z., Mitov, I., Iliev, M.: Study on the composition of biogenic iron-containing materials obtained under cultivation of the Leptothrix sp. on different media. Appl. Biochem. Biotechnol. 181, 867–883 (2017)CrossRefGoogle Scholar
  17. 17.
    Cornell R., Schwertmann, U.: The Iron Oxides: Structure, Properties, Reactions, Occurrences and Uses, (2006), Science.Google Scholar
  18. 18.
    Lieske, R.: Zur ernährungsphysiologie der Eisenbakterien. Z. Bakt. Parasitenk. Infektionskr. Hyg. Abt. II(49), 413–425 (1919)Google Scholar
  19. 19.
    Niemantsverdriet, J.W., Van der Kraan, A.M., Delgass, W.N., Vannice, M.A.: Small-particle effects in Mössbauer spectra of a carbon-supported iron catalyst. J. Phys. Chem. 89, 67–72 (1985)CrossRefGoogle Scholar
  20. 20.
    Gotić, M., Music, S.: Mossbauer, FT-IR and FE SEM investigation of iron oxides precipitated from FeSO4 solutions. J. Molec. Struct. 834–836, 445–453 (2007)ADSCrossRefGoogle Scholar
  21. 21.
    Gehring, A.U., Hofmeister, A.M.: The transformation of lepidocrocite during heating: a magnetic and spectroscopic study. Clays Clay Miner. 42, 409–415 (1994)ADSCrossRefGoogle Scholar
  22. 22.
    Lazaroff, N., Sigal, W., Wasserman, A.: Iron Oxidation and Precipitation of Ferric Hydroxysulfates by Resting Thiobacillus ferrooxidans Cells. Appl. Environ. Microbiol. 43, 924–938 (1982)Google Scholar
  23. 23.
    Weckler, B., Lutz, H.D.: Lattice vibration spectra. Part XCV. Infrared spectroscopic studies on the iron oxide hydroxides goethite (α), akaganéite (β), lepidocrocite (γ), and feroxyhite (δ). Eur. J. Solid State Inorg. Chem. 35, 531–544 (1998)CrossRefGoogle Scholar
  24. 24.
    Little, L.H.: Infrared spectra of adsorbed species. Academic Press Inc., London New York (1966)Google Scholar
  25. 25.
    Belami, L.: Infrared Spectra of Complex Molecules, J. Wiley, New York (1961)Google Scholar
  26. 26.
    Rubio, C., Ott, C., Amiel, C., Dupont-Moral, I., Travert, J., Mariey, L.: Sulfato/thiosulfato reducing bacteria characterization by FT-IR spectroscopy: a new approach to biocorrosion control. J. Microbiol. Methods. 64, 287–296 (2006)CrossRefGoogle Scholar

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© Springer International Publishing AG, part of Springer Nature 2017

Authors and Affiliations

  1. 1.Institute of CatalysisBulgarian Academy of Sciences1113 SofiaBulgaria
  2. 2.Faculty of BiologySt. Kliment Ohridski University of Sofia1164 SofiaBulgaria
  3. 3.Institute of General and Inorganic ChemistryBulgarian Academy of Sciences1113 SofiaBulgaria

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