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Hyperfine Interactions

, Volume 185, Issue 1–3, pp 103–110 | Cite as

Conformational changes in hemoglobin triggered by changing the iron charge

  • S. Croci
  • K. Achterhold
  • I. Ortalli
  • F. G. Parak
Article

Abstract

In this work the hemoglobin conformational changes induced by changing the iron charge have been studied and compared with Myoglobin. Mössbauer spectroscopy was used to follow the change of the iron conformation. In order to compare the conformational relaxation of hemoglobin and myoglobin, and to study a possible influence of the quaternary structure, an intermediate metastable state of hemoglobin has been created by low temperature X-ray irradiation of methemoglobin. The irradiation reduces the Fe(III) of the heme groups to Fe(II) Low Spin, where the water is still bound on the sixth coordination. Heating cycles performed at temperatures from 140 K to 200 K allow the molecules to overcome an activation energy barrier and to relax into a stable conformation such as deoxy-hemoglobin or carboxy-hemoglobin, if CO is present. Slightly different structures (conformational substates) reveal themselves as a distribution of energy barriers (ΔG#). The distribution of the activation energy, for the decay of the Fe(II) Low Spin intermediate, has been fitted with a Gaussian. For comparison, published myoglobin data were re-analysed in the same way. The average energy value at characteristic temperature is very similar in case of myoglobin and hemoglobin. The larger Gaussian energy distribution for myoglobin with respect to hemoglobin shows that more conformational substates are available. This may be caused by a larger area exposed to water. In hemoglobin, part of the surface of the chains is not water accessible due to the quaternary structure.

Keywords

Conformational changes Hemoglobin Free activation energy 

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Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • S. Croci
    • 1
  • K. Achterhold
    • 2
  • I. Ortalli
    • 1
  • F. G. Parak
    • 2
  1. 1.Departments of Public Health, INBB ParmaUniversity of ParmaParmaItaly
  2. 2.Physik-Department E17GarchingGermany

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