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Feeding rates and prey selection of the invasive Asian clam, Corbicula fluminea, on microplankton in the Columbia River, USA

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Abstract

The Asian clam, Corbicula fluminea, was introduced into North America in the 1920s—first observed in the Columbia River—and has expanded its range across the continent and into South America and Europe, yet little is known about its ecology and potential to impact food webs. To evaluate prey selectivity and feeding rates of C. fluminea, we conducted laboratory feeding experiments using water from two distinct Columbia River environments (unimpounded river and reservoir) during July and October 2016. The mean clearance rate on microplankton was 270 (± 53.6 SE) ml water clam−1 h−1 and mean ingestion rate was 2.45 (± 0.83 SE) µg C clam−1 h−1, although rates varied with season and location. In the reservoir in July, clams preferred diatoms and showed an avoidance of dinoflagellates and flagellates; during October in the unimpounded river, clams preferred flagellates while showing a significant avoidance of cyanobacteria. Diatoms were dominant at both sites, and were ingested by clams; however, clams ingested cyanobacteria at very low rates. Substantial consumption of microplankton such as diatoms and rejection of cyanobacteria by C. fluminea may provide competitive advantages to cyanobacteria, leading to microplankton community composition shifts and other changes to food webs in the Columbia River.

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References

  • Ahlgren, G., L. Lundstedt, M. Brett & C. Forsberg, 1990. Lipid composition and food quality of some freshwater phytoplankton for cladoceran zooplankters. Journal of Plankton Research 12: 809–818.

    Article  CAS  Google Scholar 

  • Atkinson, C. L., M. R. First, A. P. Covich, S. P. Opsahl & S. W. Golladay, 2011. Suspended material availability and filtration-biodeposition processes performed by a native and invasive bivalve species in streams. Hydrobiologia 667: 191–204.

    Article  CAS  Google Scholar 

  • Baker, S. M., J. S. Levinton, J. P. Kurdziel & S. E. Shumway, 1998. Selective feeding and biodeposition by zebra mussels and their relation to changes in phytoplankton composition and seston load. Journal of Shellfish Research 17: 1207–1214.

    Google Scholar 

  • Bastviken, D. T. E., N. F. Caraco & J. J. Cole, 1998. Experimental measurements of zebra mussel (Dreissena polymorpha) impacts on phytoplankton community composition. Freshwater Biology 39: 375–386.

    Article  Google Scholar 

  • Beaver, J. R., T. L. Crisman & R. J. Brock, 1991. Grazing effects of an exotic bivalve (Corbicula fluminea) on hypereutrophic lake water. Lake and Reservoir Management 7: 45–51.

    Article  Google Scholar 

  • Bollens, S. M., J. R. Cordell, S. Avent & R. Hooff, 2002. Zooplankton invasions: a brief review, plus two case studies from the northeast Pacific Ocean. Hydrobiologia 480: 87–110.

    Article  Google Scholar 

  • Bollens, S., J. Breckenridge, J. Cordell, G. Rollwagen-Bollens & O. Kalata, 2012. Invasive copepods in the Lower Columbia River Estuary: seasonal abundance, co-occurrence and potential competition with native copepods. Aquatic Invasions 7: 101–109.

    Article  Google Scholar 

  • Boltovskoy, D., I. Izaguirre & N. Correa, 1995. Feeding selectivity of Corbicula fluminea (Bivalvia) on natural phytoplankton. Hydrobiologia 312: 171–182.

    Article  Google Scholar 

  • Boltovskoy, D., N. Correa, F. Bordet, V. Leites & D. Cataldo, 2013. Toxic Microcystis (cyanobacteria) inhibit recruitment of the bloom-enhancing invasive bivalve Limnoperna fortunei. Freshwater Biology 58: 1968–1981.

    Article  Google Scholar 

  • Bowen, A., G. Rollwagen-Bollens, S. M. Bollens & J. Zimmerman, 2015. Feeding of the invasive copepod Pseudodiaptomus forbesi on natural microplankton assemblages within the lower Columbia River. Journal of Plankton Research 37: 1089–1094.

    Google Scholar 

  • Breckenridge, J. K., S. M. Bollens, G. Rollwagen-Bollens & G. C. Roegner, 2015. Plankton assemblage variability in a river-dominated temperate estuary during late spring (high-flow) and late summer (low-flow) periods. Estuaries and Coasts 38: 93–103.

    Article  CAS  Google Scholar 

  • Caraco, N. F., J. J. Cole, P. A. Raymond, D. L. Strayer, M. L. Pace, S. E. G. Findlay & D. T. Fischer, 1997. Zebra mussel invasion in a large, turbid river: phytoplankton response to increased grazing. Ecology 78: 588–602.

    Article  Google Scholar 

  • Cataldo, D., A. Vinocur, I. O´Farrell, E. M. Paolucci, V. Leites & D. Boltovskoy, 2012. The introduced bivalve Limnoperna fortunei boosts Microcystis growth in Salto Grande Reservoir (Argentina): evidence from mesocosm experiments. Hydrobiologia 680: 25–38.

    Article  CAS  Google Scholar 

  • Cohen, R. R. H., P. V. Dresler, E. J. P. Phillips & R. L. Cory, 1984. The effect of the Asiatic clam, Corbicula fluminea, on phytoplankton of the Potomac River, Maryland. Limnology and Oceanography 29: 170–180.

    Article  Google Scholar 

  • Confer, J. L. & M. V. Moore, 1987. Interpreting selectivity indices calculated from field data or conditions of prey replacement. Canadian Journal of Fisheries and Aquatic Sciences 44: 1529–1533.

    Article  Google Scholar 

  • Cordell, J. R., S. M. Bollens, R. Draheim & M. Systma, 2008. Asian copepods on the move: recent invasions in the Columbia-Snake River system, USA. ICES Journal of Marine Science 65: 753–758.

    Article  Google Scholar 

  • Counts III, C. L., 1981. Corbicula fluminea (Bivalvia: sphaeriacea) in British Columbia. Nautilus 95: 12–13.

    Google Scholar 

  • Dexter, E., S. M. Bollens, G. Rollwagen-Bollens, J. Emerson & J. Zimmerman, 2015. Persistent vs. ephemeral invasions: 8.5 years of zooplankton community dynamics in the Columbia River. Limnology and Oceanography 60: 527–539.

    Article  Google Scholar 

  • Dexter, E., S. M. Bollens, J. Cordell, H. Y. Soh, G. Rollwagen-Bollens, S. Pfeifer, J. Goudet & S. Vuilleumier, 2018. A genetic reconstruction of the invasion of the calanoid copepod Pseudodiaptomus inopinus across the North American Pacific Coast. Biological Invasions 20: 1577–1595.

    Article  Google Scholar 

  • Dionisio Pires, L. M. & E. Van Donk, 2002. Comparing grazing by Dreissena polymorpha on phytoplankton in the presence of toxic and non-toxic cyanobacteria. Freshwater Biology 47: 1855–1865.

    Article  Google Scholar 

  • Dionisio Pires, L. M., R. R. Jonker, E. Van Donk & H. J. Laanbroek, 2004. Selective grazing by adults and larvae of the zebra mussel (Dreissena polymorpha (Pallas)): application of flow cytometry to natural seston. Freshwater Biology 49: 116–126.

    Article  Google Scholar 

  • Emerson, J. E., S. M. Bollens & T. D. Counihan, 2015. Seasonal dynamics of zooplankton in Columbia-Snake River reservoirs, with special emphasis on the invasive copepod Pseudodiaptomus forbesi. Aquatic Invasions 10: 25–40.

    Article  Google Scholar 

  • Fahnenstiel, G., S. Pothoven, H. Vanderploeg, D. Klarer, T. Nalepa & D. Scavia, 2010. Recent changes in primary production and phytoplankton in the offshore region of southeastern Lake Michigan. Journal of Great Lakes Research 36: 20–29.

    Article  Google Scholar 

  • Gosselain, V., J.-P. Descy & E. Everbecq, 1994. The phytoplankton community of the River Meuse, Belgium: seasonal dynamics (year 1992) and the possible incidence of zooplankton grazing. Hydrobiologia 289: 179–191.

    Article  Google Scholar 

  • Havens, K. E., 2008. Cyanobacteria blooms: effects on aquatic ecosystems. Advances in Experimental Medicine and Biology 619: 733–747.

    Article  Google Scholar 

  • Hakenkamp, C. C., S. G. Ribblett, M. A. Palmer, C. M. Swan, J. W. Reid & M. R. Goodison, 2001. The impact of an introduced bivalve (Corbicula fluminea) on the benthos of a sandy stream. Freshwater Biology 46: 491–501.

    Article  Google Scholar 

  • Hassett, W., S. M. Bollens, T. D. Counihan, G. Rollwagen-Bollens, J. Zimmerman & J. Emerson, 2017. Veligers of the invasive Asian clam Corbicula fluminea in the Columbia River Basin: broadscale distribution, abundance and ecological associations. Lake and Reservoir Management 33: 234–248.

    Article  Google Scholar 

  • Holz, J. C., K. D. Hoagland, R. L. Spawn, A. Popp & J. L. Andersen, 1997. Phytoplankton community response to reservoir aging, 1968–92. Hydrobiologia 346: 183–192.

    Article  CAS  Google Scholar 

  • Horgan, M. J. & E. L. Mills, 1997. Clearance rates and filtering activity of zebra mussel (Dreissena polymorpha): implications for freshwater lakes. Canadian Journal of Fisheries and Aquatic Sciences 54: 249–255.

    Article  Google Scholar 

  • Ituarte, C. F., 1994. Corbicula and Neocorbicula (Bivalvia: Corbiculidae) in the Paraná, Uruguay, and Rio de la Plata Basins. Nautilus 107: 129–135.

    Google Scholar 

  • Jacoby, J. M. & J. Kann, 2007. The occurrence and response to toxic cyanobacteria in the Pacific Northwest, North America. Lake and Reservoir Management 23: 123–143.

    Article  Google Scholar 

  • Juhel, G., J. Davenport, J. O’Halloran, S. C. Culloty, R. M. O’Riordan, K. F. James, A. Furey & O. Allis, 2006. Impacts of microcystins on the feeding behavior and energy balance of zebra mussels, Dreissena polymorpha: a bioenergetics approach. Aquatic Toxicology 79: 391–400.

    Article  CAS  Google Scholar 

  • Karatayev, A. Y., L. E. Burlakova & D. K. Padilla, 1997. The effects of Dreissena polymorpha (Pallas) invasion on aquatic communities in Eastern Europe. Journal of Shellfish Research 16: 187–203.

    Google Scholar 

  • Knoll, L. B., O. Sarnelle, S. K. Hamilton, C. E. H. Kissman, A. E. Wilson, J. B. Rose & M. R. Morgan, 2008. Invasive zebra mussels (Dreissena polymorpha) increase cyanobacterial toxin concentrations in low-nutrient lakes. Canadian Journal of Fisheries and Aquatic Sciences 64: 448–455.

    Article  Google Scholar 

  • Komaru, A., M. Yamada & S. Houki, 2013. Relationship between two androgenetic clam species, Corbicula leana and Corbicula fluminea, inferred from mitochondrial cytochrome b and nuclear 28S rRNA markers. Zoological Science 30: 360–365.

    Article  Google Scholar 

  • Lauritsen, D. D., 1986. Filter-feeding in Corbicula fluminea and its effect on seston removal. Journal of the North American Benthological Society 5: 165–172.

    Article  Google Scholar 

  • Lee, T. A., G. Rollwagen-Bollens, S. M. Bollens & J. Faber-Hammond, 2015a. Environmental influence on cyanobacteria abundance and microcystin toxin production in a shallow temperate lake. Ecotoxicology and Environmental Safety 114: 318–325.

    Article  CAS  Google Scholar 

  • Lee, T. A., G. C. Rollwagen-Bollens & S. M. Bollens, 2015b. The influence of water quality variables on cyanobacteria blooms and phytoplankton community composition in a shallow temperate lake. Environmental Monitoring and Assessment 187: 315.

    Article  Google Scholar 

  • Lee, T. A., S. M. Bollens, G. Rollwagen-Bollens & J. E. Emerson, 2016. The effects of eutrophication and invasive species on zooplankton community dynamics in a shallow temperate lake. Fundamental and Applied Limnology 188: 215–231.

    Article  Google Scholar 

  • Li, J., S. Dong, S. Liu, Z. Yang, M. Peng & C. Zhao, 2013. Effects of cascading hydropower dams on the composition, biomass and biological integrity of phytoplankton assemblages in the middle Lancang-Mekong River. Ecological Engineering 60: 316–324.

    Article  Google Scholar 

  • Ludyanskiy, M. L., D. McDonald & D. MacNeill, 1993. Impact of the zebra mussel, a bivalve invader. BioScience 43: 533–544.

    Article  Google Scholar 

  • Marin, V., M. E. Huntley & B. Frost, 1986. Measuring feeding rates of pelagic herbivores: analysis of experimental design and methods. Marine Biology 93: 49–58.

    Article  Google Scholar 

  • McMahon, R. F., 1983. Ecology of an invasive pest Bivalve, Corbicula. The Mollusca 6: 505–561.

    Google Scholar 

  • Menden-Deuer, S. & E. J. Lessard, 2000. Carbon to volume relationships for dinoflagellates, diatoms, and other protist plankton. Limnology and Oceanography 45: 569–579.

    Article  CAS  Google Scholar 

  • Moal, J., V. Martin-Jezequel, R. P. Harris, J.-F. Samain & S. A. Poulet, 1987. Interspecific and intraspecific variability of the chemical composition of marine phytoplankton. Oceanologica Acta 10: 339–346.

    CAS  Google Scholar 

  • Moustaka-Gouni, M., E. Vardaka, E. Michaloudi, K. A. Kormas, E. Tryfon, H. Mihalatou, S. Gkelis & T. Lanaras, 2006. Plankton food web structure in a eutrophic polymictic lake with a history of toxic cyanobacterial blooms. Limnology and Oceanography 51: 715–727.

    Article  Google Scholar 

  • Mouthon, J., 1981. Sur la présence en France et au Portugal de Corbicula (Bivalvia, Corbiculidae) originaire d’Asie. Basteria 45: 109–116.

    Google Scholar 

  • Naddafi, R., K. Pettersson & P. Eklöv, 2007. The effect of seasonal variation in selective feeding by zebra mussels (Dreissena polymorpha) on phytoplankton community composition. Freshwater Biology 52: 823–842.

    Article  Google Scholar 

  • Olson, M., E. Lessard, C. Wong & M. Bernhardt, 2006. Copepod feeding selectivity on microplankton, including the toxigenic diatoms Pseudo-nitzschia spp., in the coastal Pacific Northwest. Marine Ecology Progress Series 326: 207–220.

    Article  Google Scholar 

  • Paerl, H. W. & T. G. Otten, 2013. Harmful cyanobacterial blooms: causes, consequences and controls. Microbial Ecology 65: 995–1010.

    Article  CAS  Google Scholar 

  • Paolucci, E. M., E. V. Thuesen, D. H. Cataldo & D. Boltovskoy, 2010. Veligers of an introduced bivalve, Limnoperna fortunei, are a new food resource that enhances growth of larval fish in the Paraná River (South America). Freshwater Biology 55: 1831–1844.

    Article  Google Scholar 

  • Payne, J. T., A. W. Wood, A. F. Hamlet, R. N. Palmer & D. P. Lettenmaier, 2004. Mitigating the effects of climate change on the water resources of the Columbia River basin. Climatic Change 62: 233–256.

    Article  Google Scholar 

  • Pham, T.-L., K. Shimizu, T.-S. Dao, L.-C. Hong-Do & M. Utsumi, 2015. Microcystin uptake and biochemical responses in the freshwater clam Corbicula leana P. exposed to toxic and non-toxic Microcystis aeruginosa: evidence of tolerance to cyanotoxins. Toxicology Reports 2: 88–98.

    Article  CAS  Google Scholar 

  • Pigneur, L.-M., E. Falisse, K. Roland, E. Everbecq, J.-F. Deliège, J. S. Smitz, K. Doninck & J.-P. Descy, 2014. Impact of invasive Asian clams, Corbicula spp., on a large river ecosystem. Freshwater Biology 59: 573–583.

    Article  Google Scholar 

  • Rahel, F. J., 2002. Homogenization of freshwater fauna. Annual Review of Ecology and Systematics 33: 291–315.

    Article  Google Scholar 

  • Rojas Molina, F. & S. J. de Paggi, 2008. Zooplankton in the Paraná River floodplain (South America) before and after the invasion of Limnoperna fortunei (Bivalvia). Wetlands 28: 695–702.

    Article  Google Scholar 

  • Rojas Molina, F., S. J. de Paggi & D. Frau, 2012. Impacts of the invading golden mussel Limnoperna fortunei on zooplankton: a mesocosm experiment. Zoological Studies 51: 733–744.

    Google Scholar 

  • Rollwagen-Bollens, G., S. M. Bollens, A. Gonzalez, J. Zimmerman, T. Lee & J. Emerson, 2013. Feeding dynamics of the copepod Diacyclops thomasi before, during and following filamentous cyanobacteria blooms in a large, shallow temperate lake. Hydrobiologia 705: 101–118.

    Article  CAS  Google Scholar 

  • Rollwagen-Bollens, G., T. Lee, V. Rose & S. M. Bollens, 2018. Beyond eutrophication: Vancouver Lake, WA, USA as a model system for assessing multiple, interacting biotic and abiotic drivers of harmful cyanobacterial blooms. Water 10: 757.

    Article  Google Scholar 

  • Rose, V. J., W. M. Forney, R. A. Norton & J. A. Harrison, 2019. Catchment characteristics, water quality, and cyanobacterial blooms in Washington and Oregon lakes. Lake & Reservoir Management 5: 4. https://doi.org/10.1080/10402381.2018.1518940.

    Article  CAS  Google Scholar 

  • Sabater, S., J. Artigas, C. Durán, M. Pardos, A. M. Romaní, E. Tornés & I. Ylla, 2008. Longitudinal development of chlorophyll and phytoplankton assemblages in a regulated large river (the Ebro River). Science of the Total Environment 404: 196–206.

    Article  CAS  Google Scholar 

  • Sala, O. E., F. S. Chapin III, J. J. Armesto, E. Berlow, J. Bloomfield, R. Dirzo, E. Huber-Sanwald, L. F. Huenneke, R. B. Jackson, A. Kinzig, R. Leemans, D. M. Lodge, H. A. Mooney, M. Oesterheld, N. L. Poff, M. T. Sykes, B. H. Walker, M. Walker & D. H. Wall, 2000. Global biodiversity scenarios for the year 2100. Science 287: 1770–1774.

    Article  CAS  Google Scholar 

  • Simenstad, C. A., L. F. Small, C. D. McIntire, D. A. Jay & C. Sherwood, 1990. Columbia River estuary studies: an introduction to the estuary, a brief history, and prior studies. Progress in Oceanography 25: 1–13.

    Article  Google Scholar 

  • Smits, A., A. Litt, J. R. Cordell, O. Kalata & S. M. Bollens, 2013. Non-native cladoceran Bosmina coregoni established on the Pacific coast of North America. Bioinvasion Records 2: 281–286.

    Article  Google Scholar 

  • Sousa, R., L. Guilhermino & C. Antunes, 2005. Molluscan fauna in the freshwater tidal area of the River Minho estuary, NW of Iberian Peninsula. Annales de Limnologie 41: 141–147.

    Article  Google Scholar 

  • Strickland, J. D. H. & T. R. Parsons, 1972. A practical manual for seawater analysis. Fisheries Research Board of Canada Bulletin 167.

  • Tang, H., H. A. Vanderploeg, T. H. Johengen & J. R. Liebig, 2014. Quagga mussel (Dreissena rostriformis bugensis) selective feeding of phytoplankton in Saginaw Bay. Journal of Great Lakes Research 40: 83–94.

    Article  Google Scholar 

  • Urrutia-Cordero, P., M. K. Ekvall & L. Hansson, 2015. Responses of cyanobacteria to herbivorous zooplankton across predator regimes: who mows the bloom? Freshwater Biology 60: 960–972.

    Article  Google Scholar 

  • Vanderploeg, H. A. & D. Scavia, 1979. Two electivity indices for feeding with special reference to zooplankton grazing. Journal of the Fisheries Research Board of Canada 36: 362–365.

    Article  Google Scholar 

  • Vanderploeg, H. A., J. R. Liebig, W. W. Carmichael, M. A. Agy, T. H. Johengen, G. L. Fahnenstiel & T. F. Nalepa, 2001. Zebra mussel (Dreissena polymorpha) selective filtration promoted toxic Microcystis blooms in Saginaw Bay (Lake Huron) and Lake Erie. Canadian Journal of Fisheries and Aquatic Sciences 58: 1208–1221.

    Article  CAS  Google Scholar 

  • Viergutz, C., C. Linn & M. Weitere, 2012. Intra- and interannual variability surpasses direct temperature effects on the clearance rates of the invasive clam Corbicula fluminea. Marine Biology 159: 2379–2387.

    Article  Google Scholar 

  • Vohmann, A., J. Borcherding, A. Kureck, A. Vaate, A. de Bij, H. Arndt & M. Weitere, 2010. Strong body mass decrease of the invasive clam Corbicula fluminea during summer. Biological Invasions 12: 53–64.

    Article  Google Scholar 

  • Way, C. M., D. J. Hornbach, T. Deneka & R. A. Whitehead, 1989. A description of the ultrastructure of the gills of freshwater bivalves, including a new structure, the frontal cirrus. Canadian Journal of Zoology 67: 357–362.

    Article  Google Scholar 

  • Way, C. M., D. J. Hornbach, C. A. Miller-Way, B. S. Payne & A. C. Miller, 1990. Dynamics of filter feeding in Corbicula fluminea (Bivalvia: Corbiculidae). Canadian Journal of Zoology 68: 115–120.

    Article  Google Scholar 

  • Wehr, J. D. & R. G. Sheath (eds), 2002. Freshwater Algae of North America: Ecology and Classification. Academic Press, San Diego.

    Google Scholar 

  • Wong, W. H., T. Tietjen, S. Gerstenberger, G. C. Holdren, S. Mueting, E. Loomis, P. Roefer, B. Moore, K. Turner & I. Hannoun, 2010. Potential ecological consequences of invasion of the quagga mussel (Dreissena bugensis) into Lake Mead, Nevada-Arizona. Lake and Reservoir Management. 26: 306–331.

    Article  CAS  Google Scholar 

  • Zar, J., 1996. Biostatistical Analysis, 3rd ed. Prenctice Hall, New Jersey.

    Google Scholar 

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Acknowledgements

This research was supported in part by the National Science Foundation’s Research Experience for Undergraduates program (grant # DBI-1461057 to GRB and SMB) and the Murdock Charitable Trust’s Partners in Science program (grant # PIS-2016337 to SMB).

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  1. School of the Environment, Washington State University, 14204 NE Salmon Creek Avenue, Vancouver, WA, 98686-9600, USA

    Benjamin A. Bolam, Gretchen Rollwagen-Bollens & Stephen M. Bollens

  2. School of Biological Sciences, Washington State University, 14204 NE Salmon Creek Avenue, Vancouver, WA, 98686-9600, USA

    Gretchen Rollwagen-Bollens & Stephen M. Bollens

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  1. Benjamin A. Bolam
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Bolam, B.A., Rollwagen-Bollens, G. & Bollens, S.M. Feeding rates and prey selection of the invasive Asian clam, Corbicula fluminea, on microplankton in the Columbia River, USA. Hydrobiologia 833, 107–123 (2019). https://doi.org/10.1007/s10750-019-3893-z

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