, Volume 805, Issue 1, pp 221–230 | Cite as

Occurrence of the planktonic bloom-forming marine diatom Chaetoceros tenuissimus Meunier and its infectious viruses in western Japan

  • Yuji Tomaru
  • Kensuke Toyoda
  • Kei Kimura
Primary Research Paper


The genus Chaetoceros is among the most species-rich marine planktonic diatoms. Most Chaetoceros are considered important primary producers in various marine environments, but because of their small size, we know little about their ecology and distribution. Therefore, from 2008 to 2012, we examined the occurrence of C. tenuissimus Meunier, one of the smallest members in the genus, and its infectious viruses in western Japanese coastal waters. Using real-time quantitative PCR, we found that C. tenuissimus was widely detected throughout our study sites, with a maximum concentration of 2.4 × 107 cells/l in May 2012. Sediment analysis revealed that C. tenuissimus resting-stage cells were present at potentially high levels, despite its infectious viruses being detected in the same region. The present study suggests that C. tenuissimus remains highly productive even when surrounded by its infectious viruses. This tolerance to viral infection, along with the diatom’s fast growth rate, suggests that C. tenuissimus might play an important role in maintaining the growth of important filter feeders.


Algal viruses Primary producers Marine algae Real-time quantitative PCR Resting-stage cells 



This study was supported by KAKENHI (16K14963) and grants for Scientific Research on Innovative Areas (16H06429, 16K21723, 16H06437) from the Ministry of Education, Culture, Science, Sports and Technology (MEXT) of Japan.

Author contributions

Y.T. wrote the main text and prepared all figures and tables. All authors collected data, conducted experiments, and reviewed the manuscript.

Compliance with ethical standard

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

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Supplementary material 1 (PPTX 304 kb)
10750_2017_3306_MOESM2_ESM.docx (51 kb)
Supplementary material 2 (DOCX 50 kb)


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Copyright information

© Springer International Publishing AG 2017

Authors and Affiliations

  1. 1.Japan Fisheries Research and Education AgencyNational Research Institute of Fisheries and Environment of the Inland SeaHatsukaichiJapan
  2. 2.Department of BiologyNippon Dental UniversityTokyoJapan
  3. 3.Ariake Sea Research Project/Institute of Lowland and Marine ResearchSaga UniversitySagaJapan

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