Hydrobiologia

, Volume 778, Issue 1, pp 105–120 | Cite as

Contrasting factors drive within-lake bacterial community composition and functional traits in a large shallow subtropical lake

  • Marla Sonaira Lima
  • David da Motta Marques
  • Ng Haig They
  • Katherine D. McMahon
  • Lúcia Ribeiro Rodrigues
  • Luciana de Souza Cardoso
  • Luciane Oliveira Crossetti
SHALLOW LAKES

Abstract

Lakes are considered as “islands” for assessing microbial biogeography, but bacterial community composition (BCC) and function may vary significantly within lakes, with the roles of scale and connectivity still unclear. This study investigated the spatial and temporal heterogeneity of the BCC (automated ribosomal intergenic spacer analysis) and functional traits (FT, carbon-source utilization), and the contribution of: (i) environmental variables, (ii) phytoplankton, (iii) season, and (iv) space, through variance partitioning in the large and well-mixed Lake Mangueira. The BCC and FT differed in time and space, with BCC being explained by environmental variables and phytoplankton, whereas FT was explained only by space. The smallest scale of variability detected by the BCC and FT (~49 km) was larger than scales found in the other studies, suggesting an effect of lake size (fetch and connectivity). Our results indicate that barriers to bacterial dispersal due to long distances are overcome by high connectivity, reinforcing the role of species sorting for BCC. FT were probably driven by gene dispersal and/or the effects of local conditions on migrant bacterial taxa and resuspended bacteria. Our results highlight the role of within-lake heterogeneity for ecosystem functioning and the implications for the appropriate scale to use in sampling bacterial communities.

Keywords

Automated ribosomal intergenic spacer analysis Ecoplate Within-lake biogeography Variance partitioning Species sorting Dispersal Lake Mangueira 

Supplementary material

10750_2015_2610_MOESM1_ESM.tif (1.5 mb)
Supplementary Fig. S1 Contour plots showing temporal variation of OTUs and carbon substrates that contributed most to BCC and FT dissimilarities, according to SIMPER. Black dots represent the sampling points along Lake Mangueira. (TIFF 1491 kb)
10750_2015_2610_MOESM2_ESM.tif (463 kb)
Supplementary Fig. S2 Contour plot showing spatial variation of OTUs and carbon substrates that contributed most to BCC and FT dissimilarities, according to SIMPER. Black dots represent the sampling points along the South, Center and North areas of Lake Mangueira. (TIFF 462 kb)
10750_2015_2610_MOESM3_ESM.doc (70 kb)
Supplementary material 3 (DOC 70 kb)
10750_2015_2610_MOESM4_ESM.doc (94 kb)
Supplementary material 4 (DOC 94 kb)
10750_2015_2610_MOESM5_ESM.doc (66 kb)
Supplementary material 5 (DOC 67 kb)

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Copyright information

© Springer International Publishing Switzerland 2016

Authors and Affiliations

  • Marla Sonaira Lima
    • 1
  • David da Motta Marques
    • 1
  • Ng Haig They
    • 2
  • Katherine D. McMahon
    • 3
  • Lúcia Ribeiro Rodrigues
    • 1
  • Luciana de Souza Cardoso
    • 4
  • Luciane Oliveira Crossetti
    • 5
  1. 1.Laboratório de Ecotecnologia e Limnologia Aplicada, Instituto de Pesquisas Hidráulicas - IPHUniversidade Federal do Rio Grande do Sul/UFRGSPorto AlegreBrazil
  2. 2.Laboratorio de Limnologia, Departamento de Oceanografia e LimnologiaUniversidade Federal do Rio Grande do Norte/UFRNNatalBrazil
  3. 3.Departments of Civil and Environmental Engineering and BacteriologyUniversity of Wisconsin-MadisonMadisonUSA
  4. 4.Departamento de Botânica, Instituto de BiociênciasUniversidade Federal do Rio Grande do Sul/UFRGSPorto AlegreBrazil
  5. 5.Departamento de Ecologia, Instituto de BiosciênciasUniversidade Federal do Rio Grande do Sul/UFRGSPorto AlegreBrazil

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