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Hydrobiologia

, Volume 758, Issue 1, pp 235–242 | Cite as

Comparison of different methods used for phosphorus determination in aquatic organisms

  • Gergely Boros
  • Attila Mozsár
Primary Research Paper

Abstract

The reliable determination of the total phosphorus (P) content stored in aquatic biota is essential for studies on nutrient stoichiometry, as well as for effective lake management measures. However, a variety of methods are found in the literature for sample P content determination, which renders it necessary to assess whether the data reported in different studies are comparable. We used different combinations of combustion durations, acid types and acid concentrations for sample digestion, and measured P concentrations subsequently with the standard colorimetric method. In addition, P contents of samples were assayed by ICP–OES and MP–AES methods. Our results confirmed that the variability among studies using different methods may explain some of the reported intraspecific and interspecific variation. We found that duration of combustion exerted the most important influence on the P retrieval, while acid type and acidity of the hydrolysing solution did not substantially influence the efficiency of sample digestion. We recommend using 8 h of combustion and 0.3 N HCl for acid hydrolysis prior to the colorimetric P analysis, and urge standardisation in the P analyses of biotic samples so as to obtain reliable results and data comparable among different studies.

Keywords

Fish Benthic invertebrate Zooplankton Macrophyte Phosphorus Sample digestion 

Notes

Acknowledgments

The authors would like to thank M. J. Vanni, S. Palmer, S. Harangi, E. Baranyai, Z. Vital and Z. Poller for help with laboratory work and manuscript preparation. We acknowledge the contribution of Agilent Technologies and the Novo-Lab Ltd. (Hungary) for providing the ICP-OES 720 and the MP-AES 4100.

References

  1. Benstead, J. P., J. M. Hood, N. V. Whelan, M. R. Kendrick, D. Nelson, A. F. Hanninen & L. M. Demi, 2014. Coupling of dietary phosphorus and growth across diverse fish taxa: a meta-analysis of experimental aquaculture studies. Ecology 95: 2768–2777.CrossRefGoogle Scholar
  2. Boros, G., I. Tátrai & S. A. Nagy, 2009. Using high-pressure Teflon bomb digestion in phosphorus determination of aquatic animals. International Journal of Limnology 45: 55–58.CrossRefGoogle Scholar
  3. Boros, G., J. Jyväsjärvi, P. Takács, A. Mozsár, I. Tátrai, M. Søndergaard & R. I. Jones, 2012. Between–lake variation in the elemental composition of roach (Rutilus rutilus L.). Aquatic Ecology 46: 385–394.CrossRefGoogle Scholar
  4. Brönmark, C. & L. A. Hansson, 2005. The Biology of Lakes and Ponds. Oxford University Press, Oxford.Google Scholar
  5. Carpenter, S. R., K. L. Cottingham & D. E. Schindler, 1992. Biotic feedbacks in lake phosphorus cycles. Trends in Ecology and Evolution 7: 332–336.PubMedCrossRefGoogle Scholar
  6. Claeson, S. M., J. L. Li, J. E. Compton & P. A. Bisson, 2006. Response of nutrients, biofilm, and benthic insects to salmon carcass addition. Canadian Journal of Fisheries and Aquatic Sciences 63: 1230–1241.CrossRefGoogle Scholar
  7. Czamanski, M., A. Nugraha, P. Pondaven, M. Lasbleiz, A. Masson, N. Caroff, R. Bellail & P. Tréguer, 2011. Carbon, nitrogen and phosphorus elemental stoichiometry in aquacultured and wild–caught fish and consequences for pelagic nutrient dynamics. Marine Biology 158: 2847–2862.CrossRefGoogle Scholar
  8. Dodson, S. I., 2005. Introduction to Limnology. McGraw Hill, New York.Google Scholar
  9. El–Sabaawi, R. W., T. J. Kohler, E. Zandoná, J. Travis, M. C. Marshall, S. A. Thomas, D. N. Reznick, M. Walsh, J. F. Gilliam, C. Pringle & A. S. Flecker, 2012. Environmental and organismal predictors of intraspecific variation in the stoichiometry of a neotropical freshwater fish. Plos One 7: 1–12.Google Scholar
  10. Fehér, M., E. Baranyai, E. Simon, P. Bársony, I. Szücs, J. Posta & L. Stündl, 2013. The interactive effect of cobalt enrichment in Artemia on the survival and larval growth of barramundi, Lates calcarifer. Aquaculture 414–415: 92–99.CrossRefGoogle Scholar
  11. Frost, P. C., J. P. Benstead, W. F. Cross, H. Hillebrand, J. H. Larson, M. A. Xenopoulos & T. Yoshida, 2006. Threshold elemental ratios of carbon and phosphorus in aquatic consumers. Ecology Letters 9: 774–779.PubMedCrossRefGoogle Scholar
  12. Griffiths, D., 2006. The direct contribution of fish to lake phosphorus cycles. Ecology of Freshwater Fish 15: 86–95.CrossRefGoogle Scholar
  13. Hendrixson, H. A., R. W. Sterner & A. D. Kay, 2007. Elemental stoichiometry of freshwater fishes in relation to phylogeny, allometry and ecology. Journal of Fish Biology 70: 121–140.CrossRefGoogle Scholar
  14. Kufel, L. & I. Kufel, 2002. Chara beds acting as nutrient sinks in shallow lakes – a review. Aquatic Botany 72: 249–260.CrossRefGoogle Scholar
  15. Pai, S.-C., C.-C. Yang & J. P. Riley, 1990. Effects of acidity and molybdate concentration on the kinetics of the formation of the phosphoantimonylmolybdenum blue complex. Analitica Chimica Acta 229: 115–120.CrossRefGoogle Scholar
  16. Parmenter, R. R. & V. A. Lamarra, 1991. Nutrient cycling in a freshwater marsh–the decomposition of fish and waterfowl carrion. Limnology and Oceanography 36: 976–987.CrossRefGoogle Scholar
  17. Pilati, A. & M. J. Vanni, 2007. Ontogeny, diet shifts, and nutrient stoichiometry in fish. Oikos 116: 1663–1674.CrossRefGoogle Scholar
  18. Rodushkin, I., T. Ruth & A. Huhtasaari, 1999. Comparison of two digestion methods for elemental determinations in plant material by ICP techniques. Analytica Chimica Acta 378: 191–200.CrossRefGoogle Scholar
  19. Rønsholdt, B., 1995. Effect of size/age and feed composition on body composition and phosphorus content of rainbow trout Oncorhynchus mykiss. Water Science and Technology 31: 175–183.CrossRefGoogle Scholar
  20. Shearer, K. D., 1984. Changes in elemental composition of hatchery-reared rainbow trout, Salmo gairdneri, associated with growth and reproduction. Canadian Journal of Fisheries and Aquatic Sciences 41: 1592–1600.CrossRefGoogle Scholar
  21. Sterner, R. W., 2008. On the phosphorus limitation paradigm for lakes. International Review of Hydrobiology 93: 433–445.CrossRefGoogle Scholar
  22. Sterner, R. W. & J. J. Elser, 2002. Ecological Stoichiometry: The Biology of Elements from Molecules to the Biosphere. Princeton University Press, Princeton.Google Scholar
  23. Sterner, R. W. & N. B. George, 2000. Carbon, nitrogen and phosphorus stoichiometry of cyprinid fishes. Ecology 81: 127–140.CrossRefGoogle Scholar
  24. Strickland, J. D. H. & T. R. Parsons, 1972. A Practical Handbook of Seawater Analysis. Fisheries Research Board of Canada, Ottawa.Google Scholar
  25. Tanner, D. K., E. N. Leonard & J. C. Brazner, 1999. Microwave digestion method for phosphorus determination of fish tissue. Limnology and Oceanography 44: 708–709.CrossRefGoogle Scholar
  26. Tanner, D. K., J. C. Brazner & V. J. Brady, 2000. Factors influencing carbon, nitrogen, and phosphorus content of fish from a Lake Superior coastal wetland. Canadian Journal of Fisheries and Aquatic Sciences 57: 1243–1251.CrossRefGoogle Scholar
  27. Tarvainen, M., J. Sarvala & H. Helminen, 2002. The role of phosphorus release by roach (Rutilus rutilus L.) in the water quality changes of a biomanipulated lake. Freshwater Biology 47: 2325–2336.CrossRefGoogle Scholar
  28. Vrede, T., S. Drakare, P. Eklöv, A. Hein, A. Liess, J. Olsson, J. Persson, M. Quevedo, R. Stabo & R. Svenback, 2011. Ecological stoichiometry of Eurasian perch – intraspecific variation due to size, habitat and diet. Oikos 120: 886–896.CrossRefGoogle Scholar
  29. Walve, J. & U. Larsson, 1999. Carbon, nitrogen and phosphorus stoichiometry of crustacean zooplankton in the Baltic Sea: implications for nutrient recycling. Journal of Plankton Research 21: 2309–2321.CrossRefGoogle Scholar

Copyright information

© Springer International Publishing Switzerland 2015

Authors and Affiliations

  1. 1.Balaton Limnological Institute, MTA Centre for Ecological ResearchTihanyHungary

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