Comparison of different methods used for phosphorus determination in aquatic organisms
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The reliable determination of the total phosphorus (P) content stored in aquatic biota is essential for studies on nutrient stoichiometry, as well as for effective lake management measures. However, a variety of methods are found in the literature for sample P content determination, which renders it necessary to assess whether the data reported in different studies are comparable. We used different combinations of combustion durations, acid types and acid concentrations for sample digestion, and measured P concentrations subsequently with the standard colorimetric method. In addition, P contents of samples were assayed by ICP–OES and MP–AES methods. Our results confirmed that the variability among studies using different methods may explain some of the reported intraspecific and interspecific variation. We found that duration of combustion exerted the most important influence on the P retrieval, while acid type and acidity of the hydrolysing solution did not substantially influence the efficiency of sample digestion. We recommend using 8 h of combustion and 0.3 N HCl for acid hydrolysis prior to the colorimetric P analysis, and urge standardisation in the P analyses of biotic samples so as to obtain reliable results and data comparable among different studies.
KeywordsFish Benthic invertebrate Zooplankton Macrophyte Phosphorus Sample digestion
The authors would like to thank M. J. Vanni, S. Palmer, S. Harangi, E. Baranyai, Z. Vital and Z. Poller for help with laboratory work and manuscript preparation. We acknowledge the contribution of Agilent Technologies and the Novo-Lab Ltd. (Hungary) for providing the ICP-OES 720 and the MP-AES 4100.
- Brönmark, C. & L. A. Hansson, 2005. The Biology of Lakes and Ponds. Oxford University Press, Oxford.Google Scholar
- Dodson, S. I., 2005. Introduction to Limnology. McGraw Hill, New York.Google Scholar
- El–Sabaawi, R. W., T. J. Kohler, E. Zandoná, J. Travis, M. C. Marshall, S. A. Thomas, D. N. Reznick, M. Walsh, J. F. Gilliam, C. Pringle & A. S. Flecker, 2012. Environmental and organismal predictors of intraspecific variation in the stoichiometry of a neotropical freshwater fish. Plos One 7: 1–12.Google Scholar
- Sterner, R. W. & J. J. Elser, 2002. Ecological Stoichiometry: The Biology of Elements from Molecules to the Biosphere. Princeton University Press, Princeton.Google Scholar
- Strickland, J. D. H. & T. R. Parsons, 1972. A Practical Handbook of Seawater Analysis. Fisheries Research Board of Canada, Ottawa.Google Scholar