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Hydrobiologia

, Volume 741, Issue 1, pp 33–49 | Cite as

Using an online phycocyanin fluorescence probe for rapid monitoring of cyanobacteria in Macau freshwater reservoir

  • Yijun Kong
  • Inchio LouEmail author
  • Yiyong Zhang
  • Chong U. Lou
  • Kai Meng Mok
CHALLENGES IN AQUATIC SCIENCES

Abstract

Monitoring of cyanobacteria and their toxins are traditionally conducted by cell counting, chlorophyll-a (chl-a) determination and cyanotoxin measurements, respectively. These methods are tedious, costly, time consuming, and insensitive to rapid changes in water quality and cyanobacterial abundance. We have applied and tested an online phycocyanin (PC) fluorescence probe for rapid monitoring of cyanobacteria in the Macau Storage Reservoir (MSR) that is experiencing cyanobacterial blooms. The relationships among cyanobacterial abundance, biovolume, cylindrospermopsin concentration, and PC fluorescence were analyzed using both laboratory and in-the-field studies. The performance of the probe was compared with traditional methods, and its advantages and limitations were assessed in pure and mixed cyanobacterial cultures in the laboratory. The proposed techniques successfully estimated the species including Microcystis and Cylindrospermopsis, two toxic species recently observed in the MSR. During February–November, 2010, the PC probe detected high correlations between PC and cell numbers (R 2 = 0.71). Unlike the chl-a content, which indicates only the total algal biomass, the PC pigment specifically indicates cyanobacteria. These results support the PC parameter as a reliable estimate of cyanobacterial cell number, especially in freshwater bodies where the phytoplankton community and structure are stable. Thus, the PC probe is potentially applicable to online monitoring of cyanobacteria.

Keywords

Phycocyanin fluorescence probe Online measurement Cyanobacteria Freshwater reservoir 

Notes

Acknowledgments

We thank the technical staff at Macao Water Co. Ltd. for collecting water samples, counting cyanobacterial cells and measuring chlorophyll-a concentration. Financial support from the Fundo para o Desenvolvimento das Ciências e da Tecnologia (FDCT) (grant # FDCT/016/2011/A) and Research Committee at University of Macau are gratefully acknowledged.

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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Yijun Kong
    • 1
  • Inchio Lou
    • 2
    Email author
  • Yiyong Zhang
    • 1
  • Chong U. Lou
    • 1
  • Kai Meng Mok
    • 2
  1. 1.Laboratory & Research CenterMacao Water Co. Ltd.Macau SARChina
  2. 2.Department of Civil and Environmental Engineering, Faculty of Science and TechnologyUniversity of MacauMacau SARChina

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