Patterns of biofilm formation in two streams from different bioclimatic regions: analysis of microbial community structure and metabolism
This study evaluates the sequences of biofilm colonization at two stream sites located in different bioclimatic regions (Mediterranean and Central European). Despite of the ecoregional differences, the two selected streams mainly differed by their hydrology and nutrient condition. A range of structural [microbial biomass, nutrient content, extracellular polymeric substances (EPS)-polysaccharide content] and metabolic (extracellular enzyme activities) descriptors were analyzed during the colonization period of 60 days. The succession of bacterial communities was investigated through the 16S rDNA gene analysis and taxonomical identification was used in diatom communities. Differences in algal biomass were not significant between the two stream biofilms, but the bacterial density and aminopeptidase and β-glucosidase activities were higher in the Mediterranean biofilms, probably due to greater coarse particulate organic matter (CPOM) accumulation in the latter. The colonization sequences of algae and bacteria in the biofilms were faster in the Mediterranean stream biofilms and slower and gradual in the Central European stream biofilms. Floods caused disruption in the microbial succession and re-colonization, favouring the re-appearance of early colonizing taxa and increasing the community diversity. Biofilms in the Mediterranean stream had a higher amount of early colonizing species (the diatoms Ulnaria ulna and Karayevia clevei and the β-proteobacteria group), as well as higher polysaccharide development in the extracellular polymeric substances (EPS) matrix. These differences could be an adaptation to the marked hydrological changes characteristic of Mediterranean streams. In contrast, the microbial community complexity gradually increased and nutrients and proteins largely accumulated in the Central European biofilms, reflecting the higher hydrological stability as well as the higher nutrient availability.
KeywordsBacteria Diatom Biofilm Central European Mediterranean Stream
This study has been funded by the projects CGL2007-65549/BOS, CGL2008-05618-C02-01 and Consolider-Ingenio CSD2009-00065 (SCARCE) of the Spanish Ministry of Science and Technology, and Deutscher Akademischer Austauschdienst (DAAD; Spanien-Acciones Integradas 2005).
- Aboal, M., M. A. Puig & G. Soler, 1996. Diatom assemblages in some Mediterranean temporary streams in southeastern Spain. Archiv für Hydrobiologie 136: 509–527.Google Scholar
- American Public Health Association, 1989. Standard Methods for the Examination of Water and Wastewater, 17th ed. APHA, Washington, DC.Google Scholar
- Gosselain, V., S. Campeau, M. Gevrey, M. Coste, L. Ector, F. Rimet, J. Tison, F. Delmas, Y. S. Park, S. Lek & J.-P. Descy, 2005. Diatom typology of low-impacted conditions at a multi-regional scale: combined results of multivariate analyses and SOM. In Lek, S., M. Scardi, P. F. M. Verdonschot, J.-P. Descy & Y.-S. Park (eds), Modelling Community Structure in Freshwater Ecosystems. Springer, Berlin: 317–342.CrossRefGoogle Scholar
- Grasshoff, K., M. Ehrhardt & K. Kremling (eds), 1983. Methods of Seawater Analysis. Verlag Chemie, Weinheim.Google Scholar
- Jeffrey, S. W. & G. F. Humphrey, 1975. New spectrophotometric equations for determining chlorophylls a, b, c1 and c2 in higher plants, algae and natural phytoplankton. Biochemie und Physiologie der Pflanzen 167: 191–194.Google Scholar
- Krammer, K. & H. Lange-Bertalot, 1991–1997. Bacillariophyceae, 2 (1-4). In Ettl, H., J. Gerloff, H. Heynig & D. Mollenhauer (eds), Süsswasserflora von Mitteleuropa. Fischer, Stuttgart.Google Scholar
- Lange-Bertalot, H., 2001. Navicula sensu stricto, 10 genera separated from Navicula sensu lato, Frustulia. In Lange-Bertalot, H. (ed.), Diatoms of Europe, Vol. 2. A.R.G. Gantner Verlag K.G, Ruggell, Liechtenstein: 526 pp.Google Scholar
- Manz, W., K. Wendt-Potthoff, T. R. Neu, U. Szewzyk & J. R. Lawrence, 1999. Phylogenetic composition, spatial structure, and dynamics of lotic bacterial biofilms investigated by fluorescent in situ hybridization and confocal laser scanning microscopy. Microbial Ecology 37: 225–237.PubMedCrossRefGoogle Scholar
- Muyzer, G., T. Brinkhoff, U. Nübel, C. Santegoeds, H. Schäfer & C. Wawer, 1997. Denaturing gradient gel electrophoresis (DGGE) in microbial ecology, Chap. 3.4.4. In Akkermans, A. D. L., J. D. van Elsas & F. J. de Bruijn (eds), Molecular Microbial Ecology Manual. Kluwer Academic Publishers, Dordrecht: 1–27.Google Scholar
- Romaní, A. M. & S. Sabater, 2001. Structure and activity of rock and sand biofilms in a Mediterranean stream. Ecology 82: 3232–3245.Google Scholar