Hydrobiologia

, Volume 686, Issue 1, pp 147–156 | Cite as

Stable karyotypes: a general rule for the fish of the family Prochilodontidae?

  • Maria Leandra Terencio
  • Carlos Henrique Schneider
  • Maria Claudia Gross
  • Marcelo Ricardo Vicari
  • Eliana Feldberg
Primary Research Paper

Abstract

Cytogenetic studies involving the family Prochilodontidae have shown that these fish can be characterized by a constant diploid number and a conserved karyotypic macrostructure. This study focused on comparative physical chromosomal mapping using 18S and 5S rDNA to compare the species Semaprochilodus insignis and S. taeniurus. Our results indicated the conservation of large number of conventional chromosomal markers. The molecular cytogenetic analyses of the location of the 18S rDNA indicated the maintenance of a chromosome pair bearing these sites in both species analyzed, and it appears to be a conserved character among the majority of the species of this family. The stability of the number of 5S ribosomal DNA sites and their chromosomal localization as has been reported for the Prochilodontidae was not, however, confirmed for S. insignis and S. taeniurus, as these species showed multiple specific rDNA 5S sites. As such, and in spite of the fact that a number of studies indicate that the family Prochilodontidae has a conserved karyotypic structure, the utilization of molecular tools that use chromosomal segments as markers revealed that this presumed stability cannot be extended to the genome level for the species S. insignis and S. taeniurus.

Keywords

FISH 18S rDNA 5S rDNA Comparative cytogenetics Semaprochilodus spp. Amazon River basin 

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Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • Maria Leandra Terencio
    • 1
  • Carlos Henrique Schneider
    • 1
  • Maria Claudia Gross
    • 2
  • Marcelo Ricardo Vicari
    • 3
  • Eliana Feldberg
    • 1
  1. 1.Laboratório de Genética AnimalInstituto Nacional de Pesquisas da Amazônia—INPA/CPBA, Coordenação de Pesquisas em Biologia AquáticaManausBrazil
  2. 2.Departamento de BiologiaLaboratório de Citogenômica Animal, Universidade Federal do Amazonas, Instituto de Ciências BiológicasManausBrazil
  3. 3.Departamento de Biologia EstruturalLaboratório de Citogenética e Evolução, Universidade Estadual de Ponta Grossa, Molecular e GenéticaPonta GrossaBrazil

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