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Hydrobiologia

, Volume 644, Issue 1, pp 245–259 | Cite as

Evaluating the need for acid treatment prior to δ13C and δ15N analysis of freshwater fish scales: effects of varying scale mineral content, lake productivity and CO2 concentration

  • M. VenturaEmail author
  • E. Jeppesen
Primary research paper

Abstract

In order to evaluate the need for using scale acidification to remove carbonates prior to stable isotope analysis, we compared acidified and non-acidified scales of six freshwater fish species (perch, roach, rudd, pike, tench and bream) with contrasting mineral content in their scales. Fish samples were taken from six lakes with variable trophic conditions, ranging from oligotrophic to hypertrophic, and differing in CO2 concentrations. The scale mineral content of the six species studied ranged between 31.8 and 61.3% dry weight (DW) in tench and perch, respectively. The elemental composition was characterised by high amounts of phosphorus, varying from 4.5 to 9.1% DW. The mineral fraction was dominated by apatite (range 24.4–49.2% DW), carbonates constituted a very small proportion of the total carbon content (average ± SD: 5.5 ± 1.7%). The average effect of acidification was very small for all species (average ± SD: 0.181 ± 0.122 and −0.208 ± 0.243 for carbon and nitrogen, respectively), albeit significant for five out of the six species (excepting tench that had the lowest mineral content). Linear regression slopes between acidified and untreated scales did not differ significantly from one for almost all the species and isotopes. The effects of acidification on the two isotopes were correlated with the relative carbonate content as well as with the CO2 concentration for carbon and total phosphorus for nitrogen. We conclude that the need for scale acidification depends on the different species and on the system studied, although in most cases the acidification effect will be biologically irrelevant. However, dual analysis of acidified and untreated scales may provide useful information on differences in stable isotope composition of dissolved inorganic carbon and on phytoplankton carbon fractionation generated by varying levels of CO2 availability.

Keywords

Carbon isotope Nitrogen isotope Fish scales Acidification De-calcification Stoichiometry 

Notes

Acknowledgements

We are grateful to D. Harris at the University of California for stable isotope analysis. K. Jensen, K.L. Thomsen and the late J. Stougaard-Pedersen are acknowledged for their assistance in sample collection. We are grateful to T. Buchaca, M. Rennie and two anonymous reviewers for very helpful comments on the manuscript. A.M. Poulsen assisted in editing the manuscript. M.V. was supported by a Marie Curie post-doctoral grant (MEIF-CT-2005-010554) and a Ramon y Cajal grant (Spanish Ministry of Education and Science). We also acknowledge the EU WISER project and “CLEAR” (a Villum Kann Rasmussen Centre of Excellence Project).

References

  1. Blanco, A., S. Deudero & A. Box, 2009. Muscle and scale isotopic offset of three fish species in the Mediterranean Sea: Dentex dentex, Argyrosomus regius and Xyrichtys novacula. Rapid Communications in Mass Spectrometry 23: 2321–2328.CrossRefPubMedGoogle Scholar
  2. Cerling, T. E., G. Wittemyer, H. B. Rasmussen, F. Vollrath, C. E. Cerling, T. J. Robinson & I. Douglas-Hamilton, 2006. Stable isotopes in elephant hair document migration patterns and diet changes. Proceedings of the National Academy of Sciences USA 103: 371–373.CrossRefGoogle Scholar
  3. Chandra, S., M. J. Vander Zanden, A. C. Heyvaert, B. C. Richards, B. C. Allen & C. R. Goldman, 2005. The effects of cultural eutrophication on the coupling between pelagic primary producers and benthic consumers. Limnology and Oceanography 50: 1368–1376.CrossRefGoogle Scholar
  4. Chasar, L. C., J. P. Chanton, C. C. Koenig & F. C. Coleman, 2005. Evaluating the effect of environmental disturbance on the trophic structure of Florida Bay, USA: multiple stable isotope analyses of contemporary and historical specimens. Limnology and Oceanography 50: 1059–1072.Google Scholar
  5. Chisholm, B. S., D. F. Nelson, K. A. Hobson, H. P. Schwarcz & M. Knyf, 1983. Carbon isotope measurement techniques for bone-collagen – notes for the archaeologist. Journal of Archaeological Science 10: 355–360.CrossRefGoogle Scholar
  6. Ehleringer, J. R., G. J. Bowen, L. A. Chesson, A. G. West, D. W. Podlesak & T. E. Cerling, 2008. Hydrogen and oxygen isotope ratios in human hair are related to geography. Proceedings of the National Academy of Sciences USA 105: 2788–2793.CrossRefGoogle Scholar
  7. Elliott, J. C., 2002. Calcium phosphate biominerals. Phosphates: Geochemical, Geobiological, and Materials Importance 48: 427–453.Google Scholar
  8. Estep, M. L. F. & S. Vigg, 1985. Stable carbon and nitrogen isotope tracers of trophic dynamics in natural-populations and fisheries of the Lahontan lake system, Nevada. Canadian Journal of Fisheries and Aquatic Sciences 42: 1712–1719.CrossRefGoogle Scholar
  9. Estrada, J. A., M. Lutcavage & S. R. Thorrold, 2005. Diet and trophic position of Atlantic bluefin tuna (Thunnus thynnus) inferred from stable carbon and nitrogen isotope analysis. Marine Biology 147: 37–45.CrossRefGoogle Scholar
  10. Fountoulakis, M. & H. W. Lahm, 1998. Hydrolysis and amino acid composition analysis of proteins. Journal of Chromatography A 826: 109–134.CrossRefPubMedGoogle Scholar
  11. Garcia-Berthou, E., C. Alcaraz, Q. Pou-Rovira, L. Zamora & G. Coenders, 2005. Introduction pathways and establishment rates of invasive aquatic species in Europe. Canadian Journal of Fisheries and Aquatic Sciences 62: 453–463.CrossRefGoogle Scholar
  12. Gerdeaux, D. & M. E. Perga, 2006. Changes in whitefish scales δ13C during eutrophication and reoligotrophication of subalpine lakes. Limnology and Oceanography 51: 772–780.Google Scholar
  13. Gorokhova, E., S. Hansson, H. Hoglander & C. M. Andersen, 2005. Stable isotopes show food web changes after invasion by the predatory cladoceran Cercopagis pengoi in a Baltic Sea bay. Oecologia 143: 251–259.CrossRefPubMedGoogle Scholar
  14. Grasshoff, K., 1983. Methods of Seawater Analysis. Verlag Chemie, Weinheim: 419 pp.Google Scholar
  15. Grey, J., G. CT, J. R. Britton & C. Harrod, 2009. Stable isotope analysis of archived roach (Rutilus rutilus) scales for retrospective study of shallow lake responses to nutrient reduction. Freshwater Biology 54: 1663–1670.CrossRefGoogle Scholar
  16. Hutchinson, J. J. & C. N. Trueman, 2006. Stable isotope analyses of collagen in fish scales: limitations set by scale architecture. Journal of Fish Biology 69: 1874–1880.CrossRefGoogle Scholar
  17. Jeppesen, E., M. Meerhoff, B. A. Jacobsen, R. S. Hansen, J. P. Jensen, T. L. Lauridsen, N. Mazzeo & W. C. Branco, 2007. Restoration of shallow lakes by nutrient control and biomanipulation – the successful strategy varies with lake size and climate. Hydrobiologia 581: 269–285.CrossRefGoogle Scholar
  18. Kelly, M. H., W. G. Hagar, T. D. Jardine & R. A. Cunjak, 2006. Nonlethal sampling of sunfish and slimy sculpin for stable isotope analysis: how scale and fin tissue compare with muscle tissue. North American Journal of Fisheries Management 26: 921–925.CrossRefGoogle Scholar
  19. Kennedy, B. P., C. P. Chamberlain, J. D. Blum, K. H. Nislow & C. L. Folt, 2005. Comparing naturally occurring stable isotopes of nitrogen, carbon, and strontium as markers for the rearing locations of Atlantic salmon (Salmo salar). Canadian Journal of Fisheries and Aquatic Sciences 62: 48–57.CrossRefGoogle Scholar
  20. Lee-thorp, J. A., J. C. Sealy & N. J. Vandermerwe, 1989. Stable carbon isotope ratio differences between bone-collagen and bone apatite, and their relationship to diet. Journal of Archaeological Science 16: 585–599.CrossRefGoogle Scholar
  21. Legendre, P. & L. Legendre, 1998. Numerical Ecology. Elsevier Science, Amsterdam: 853 pp.Google Scholar
  22. Morbey, Y. E., K. Vascotto & B. J. Shuter, 2007. Dynamics of piscivory by lake trout following a smallmouth bass invasion: a historical reconstruction. Transactions of the American Fisheries Society 136: 477–483.CrossRefGoogle Scholar
  23. Perga, M. E. & D. Gerdeaux, 2003. Using the delta C-13 and delta N-15 of whitefish scales for retrospective ecological studies: changes in isotope signatures during the restoration of Lake Geneva, 1980–2001. Journal of Fish Biology 63: 1197–1207.CrossRefGoogle Scholar
  24. Perga, M. E. & D. Gerdeaux, 2004. Changes in the δ13C of pelagic food webs: the influence of lake area and trophic status on the isotopic signature of whitefish (Coregonus lavaretus). Canadian Journal of Fisheries and Aquatic Sciences 61: 1485–1492.CrossRefGoogle Scholar
  25. Peterson, B. J. & B. Fry, 1987. Stable isotopes in ecosystem studies. Annual Review of Ecology and Systematics 18: 293–320.CrossRefGoogle Scholar
  26. Pruell, R. J., B. K. Taplin & K. Cicchelli, 2003. Stable isotope ratios in archived striped bass scales suggest changes in trophic structure. Fisheries Management and Ecology 10: 329–336.CrossRefGoogle Scholar
  27. Rennie, M. D., W. G. Sprules & T. B. Johnson, 2009. Resource switching in fish following a major food web disruption. Oecologia 159: 789–802.CrossRefPubMedGoogle Scholar
  28. Satterfield, F. R. & B. P. Finney, 2002. Stable isotope analysis of Pacific salmon: insight into trophic status and oceanographic conditions over the last 30 years. Progress in Oceanography 53: 231–246.CrossRefGoogle Scholar
  29. Seshaiya, R. V., P. Ambujabai & M. Kalyani, 1963. Amino acid composition of ichtylepidin from fish scales. In Ramachandran, G. N. (ed.), Aspects of Protein Structure. Proceedings of a Symposium Held in Madras, 14–18 January, University of Madras, Madras. Academic Press, New York: 343–349.Google Scholar
  30. Sinnatamby, R. N., J. E. Bowman, J. B. Dempson & M. Power, 2007. An assessment of de-calcification procedures for delta C-13 and delta N-15 analysis of yellow perch, walleye and Atlantic salmon scales. Journal of Fish Biology 70: 1630–1635.CrossRefGoogle Scholar
  31. Sinnatamby, R. N., J. B. Dempson & M. Power, 2008. A comparison of muscle- and scale-derived delta C-13 and delta N-15 across three life-history stages of Atlantic salmon, Salmo salar. Rapid Communications in Mass Spectrometry 22: 2773–2778.CrossRefPubMedGoogle Scholar
  32. Solomon, C. T., P. K. Weber, J. J. Cech, B. L. Ingram, M. E. Conrad, M. V. Machavaram, A. R. Pogodina & R. L. Franklin, 2006. Experimental determination of the sources of otolith carbon and associated isotopic fractionation. Canadian Journal of Fisheries and Aquatic Sciences 63: 79–89.CrossRefGoogle Scholar
  33. Syväranta, J., S. Vesala, M. Rask, J. Ruuhijärvi & R. I. Jones, 2008. Evaluating the utility of stable isotope analyses of archived freshwater sample materials. Hydrobiologia 600: 121–130.CrossRefGoogle Scholar
  34. Tohse, H. & Y. Mugiya, 2008. Sources of otolith carbonate: experimental determination of carbon incorporation rates from water and metabolic CO2, and their diel variations. Aquatic Biology 1: 259–268.CrossRefGoogle Scholar
  35. Vadeboncoeur, Y., E. Jeppesen, M. J. Vander Zanden, H.-H. Scierup, K. Christoffersen & D. M. Lodge, 2003. From Greenland to green lakes: cultural eutrophication and the loss of benthic pathways in lakes. Limnology and Oceanography 48: 1408–1418.CrossRefGoogle Scholar
  36. Vander Zanden, M. J., C. R. Goldman, S. Chandra, B. C. Allen, J. E. Reuter & C. R. Goldman, 2003. Historical food web structure and restoration of native aquatic communities in the Lake Tahoe (California-Nevada) basin. Ecosystems 6: 274–288.CrossRefGoogle Scholar
  37. Vander Zanden, M. J., Y. Vadeboncoeur, M. W. Diebel & E. Jeppesen, 2005. Primary consumer stable nitrogen isotopes as indicators of nutrient source. Environmental Science & Technology 39: 7509–7515.CrossRefGoogle Scholar
  38. Ventura, M., 2006. Linking biochemical and elemental composition of freshwater and marine crustacean zooplankton. Marine Ecology Progress Series 327: 233–246.CrossRefGoogle Scholar
  39. Ventura, M. & E. Jeppesen, 2009. Effects of fixation on freshwater invertebrate carbon and nitrogen isotope composition and its arithmetic correction. Hydrobiologia 632: 297–308.CrossRefGoogle Scholar
  40. Ventura, M., L. Liboriussen, T. Lauridsen, M. Søndergaard, M. Søndergaard & E. Jeppesen, 2008. Effects of increased temperature and nutrient enrichment on the stoichiometry of primary producers and consumers in temperate shallow lakes. Freshwater Biology 53: 1434–1452.CrossRefGoogle Scholar
  41. Wainright, S. C., M. J. Fogarty, R. C. Greenfield & B. Fry, 1993. Long-term changes in the Georges Bank food web – trends in stable isotopic compositions of fish scales. Marine Biology 115: 481–493.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  1. 1.National Environmental Research InstituteAarhus UniversitySilkeborgDenmark
  2. 2.Biogeodynamics and Biodiversity Group (CSIC-UB), Centre for Advanced Studies of Blanes (CEAB)Spanish Research Council (CSIC)GironaSpain
  3. 3.Institut de Recerca de l’AiguaUniversitat de BarcelonaBarcelonaSpain
  4. 4.Institute of Plant BiologyAarhus UniversityAarhus CDenmark

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