Hydrobiologia

, Volume 635, Issue 1, pp 147–155 | Cite as

Influence of Daphnia infochemicals on functional traits of Microcystis strains (Cyanobacteria)

  • Ineke van Gremberghe
  • Pieter Vanormelingen
  • Katleen Van der Gucht
  • Antoniya Mancheva
  • Sofie D’hondt
  • Luc De Meester
  • Wim Vyverman
Primary research paper

Abstract

We conducted a laboratory experiment to investigate the influence of Daphnia infochemicals on growth rate, microcystin production, colony formation and cell size of eight Microcystis strains isolated from two lakes. The strains were characterized genetically by their 16S-23S rDNA ITS sequence. The experiment was composed of four treatments: (1) a control using filtered WC medium, (2) addition of Scenedesmus obliquus culture medium filtrate, (3) addition of Daphnia magna culture medium filtrate and (4) addition of sodium octyl sulphate, a commercially available Daphnia infochemical. Our results showed that sympatric strains differed strongly for the measured functional traits, while no correlations between traits were found. Between-strain differences in growth rate, microcystin production, colony formation and cell size were generally larger than the differences in phenotypes observed between treatments. Despite this, several strains reacted to the infochemicals by changing functional trait values. Daphnia culture medium filtrate and, to a lesser extent, sodium octyl sulphate had a negative influence on the growth rate of half of the strains and stimulated microcystin production in one strain, but the latter effect was not Daphnia-specific as Scenedesmus culture medium filtrate had the same effect. Daphnia culture medium filtrate also induced colony formation in one strain. Our data suggest that Daphnia infochemicals generally have a weak influence on growth rate, microcystin production and colony formation of Microcystis strains as compared to the inter-strain variability, while existing inducible effects are highly strain-specific.

Keywords

Colony formation Daphnia Functional traits Infochemicals Microcystins Microcystis 

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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Ineke van Gremberghe
    • 1
  • Pieter Vanormelingen
    • 1
  • Katleen Van der Gucht
    • 1
  • Antoniya Mancheva
    • 1
  • Sofie D’hondt
    • 1
  • Luc De Meester
    • 2
  • Wim Vyverman
    • 1
  1. 1.Laboratory of Protistology and Aquatic EcologyGhent UniversityGhentBelgium
  2. 2.Laboratory of Aquatic Ecology and Evolutionary BiologyKatholieke Universiteit LeuvenLeuvenBelgium

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