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Hydrobiologia

, Volume 632, Issue 1, pp 167–175 | Cite as

Composition of native and introduced mtDNA lineages in Coregonus sp. in two Austrian lakes: evidence for spatio-temporal segregation of larvae?

  • B. Pamminger-LahnsteinerEmail author
  • S. Weiss
  • K. A. Winkler
  • J. Wanzenböck
Primary research paper

Abstract

We hypothesized that there is spatio-temporal genetic (mtDNA) structure of native and introduced larval whitefish (Coregonus sp.) in two Austrian lakes (Traunsee and Hallstättersee). Larval whitefish were sampled from 12 sites in each lake and screened for variation in the mtDNA NADH-1 gene. Based on the sequencing of adult fish together with existing GenBank sequences, an RFLP protocol was developed to assign haplotypes from larval samples into one of two divergent lineages. All but one site (pelagic) in Traunsee contained both haplotypes, thus there was no support for spatial segregation of mtDNA groups in that lake. However, weekly sampling from December to May in Traunsee revealed a temporal pattern, with the native haplotypes dominating in December and January before the appearance of the introduced Baltic clade. In Hallstättersee, only three of the 12 sites sampled revealed haplotypes from the introduced clade and thus spatial segregation seems operative on that lake. Our results imply that differences in the spawning ecology of the two groups maintain sufficient reproductive isolation to be reflected in distinct larval occurrence in space and time highly consistent with genetic differences on the mtDNA level. If the two lineages were highly introgressed, we would expect to find little or no correspondence between spatio-temporal patterns in larval distribution and the differentiated mtDNA lineages.

Keywords

Coregonids Coregonus renke Coregonus maraena RFLP NADH1-gene NADH3-gene 

Notes

Acknowledgements

The Institute for Limnology in Mondsee—director T. Weisse—provided the initial financial support and spatial facilities. Additional support was provided by The Austria Commission for Interdisciplinary Ecological Studies (KIÖS) of the Austrian Academy of Sciences, the Stiftungs- und Förderungsgesellschaft of the Paris-Lodron-University Salzburg, and the Austrian Science Fund (FWF L229-B12). The genetic aspects of the project were completed at the Institute of Zoology, KF-University Graz. The authors are grateful to the staff of both institutes especially to K. Maier, J. Schmidt, M. Pöckl, S. Sušnik, B. Egger, N. Duftner, St. Kölblmüller, E. Lerceteau, T.G. Kopun for their never-ending helpfulness and patience.

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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • B. Pamminger-Lahnsteiner
    • 1
    Email author
  • S. Weiss
    • 2
  • K. A. Winkler
    • 2
  • J. Wanzenböck
    • 1
  1. 1.Austrian Academy of SciencesInstitute for LimnologyMondsee Austria
  2. 2.Institute for ZoologyKarl-Franzens-UniversityGrazAustria

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