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Hydrobiologia

, Volume 641, Issue 1, pp 33–44 | Cite as

The role of interactions between Prorocentrum minimum and Heterosigma akashiwo in bloom formation

  • Y. YamasakiEmail author
  • S. Nagasoe
  • M. Tameishi
  • T. Shikata
  • Y. Zou
  • Z. Jiang
  • T. Matsubara
  • Y. Shimasaki
  • K. Yamaguchi
  • Y. Oshima
  • T. Oda
  • T. Honjo
Primary research paper

Abstract

We examined the growth and interactions between the bloom-forming flagellates Prorocentrum minimum and Heterosigma akashiwo using bi-algal culture experiments. When both species were inoculated at high cell densities, growth of H. akashiwo was inhibited by P. minimum. In other combinations of inoculation densities, the species first reaching the stationary phase substantially suppressed maximum cell densities of the other species, but the growth inhibition effect of P. minimum was stronger than that of H. akashiwo. We used a mathematical model to simulate growth and interactions of P. minimum and H. akashiwo in bi-algal cultures. The model indicated that P. minimum always out-competed H. akashiwo over time. Additional experiments showed that crude extracts from P. minimum and H. akashiwo cultures did not affect the growth of either species, but both strongly inhibited the growth of the bloom-forming diatom Skeletonema costatum. Further experiments showed that it was unlikely that reactive oxygen species produced by H. akashiwo were responsible for the inhibition of P. minimum growth.

Keywords

Allelopathy Growth and interaction Heterosigma akashiwo Prorocentrum minimum Reactive oxygen species Skeletonema costatum 

Notes

Acknowledgments

We are grateful to anonymous reviewers and the editor for thoughtful comments. This work was supported by the Grant-in-Aid for the Japan Society for the Promotion of Science (JSPS) Fellows (20-5980).

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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Y. Yamasaki
    • 1
    Email author
  • S. Nagasoe
    • 2
  • M. Tameishi
    • 3
  • T. Shikata
    • 3
  • Y. Zou
    • 1
  • Z. Jiang
    • 1
  • T. Matsubara
    • 3
  • Y. Shimasaki
    • 3
  • K. Yamaguchi
    • 1
  • Y. Oshima
    • 3
  • T. Oda
    • 1
  • T. Honjo
    • 3
  1. 1.Division of Biochemistry, Faculty of FisheriesNagasaki UniversityNagasakiJapan
  2. 2.Seikai National Fisheries Research InstituteNagasakiJapan
  3. 3.Laboratory of Marine Environmental Science, Division of Marine Biological Chemistry, Department of Bioscience and Biotechnology, Faculty of AgricultureKyushu UniversityHakozaki, FukuokaJapan

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