Strong genetic divergence indicates that congeneric damselflies Coenagrion puella and C. pulchellum (Odonata: Zygoptera: Coenagrionidae) do not hybridise
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Coenagrionid damselflies are in general decline in the British Isles. Numerous factors have been implicated in the loss of these species including recent speculation that hybridisation between congeners may result in species decline. Here we use a panel of 12 microsatellite loci to examine levels of genetic divergence and the likely occurrence of hybridisation in five populations of Coenagrion puella and C. pulchellum using samples from four sites in south-east England. Coenagrion puella and C. pulchellum were highly genetically divergent, and there was no evidence of hybridisation between any of the populations examined, even where C. puella and C. pulchellum were sympatric. There was some suggestion that C. pulchellum was less genetically diverse than C. puella, though this may have been a result of ascertainment bias associated with cross-species application of microsatellite markers. We conclude that there is no evidence that hybridisation between C. puella/pulchellum could be responsible for the on-going demographic decline in C. pulchellum. Nevertheless, further genetic studies such as this one are likely to provide estimates of diversity, population structure and dispersal capacity that will be invaluable in future conservation management strategies for coenagrionid damselflies.
KeywordsIntrogression Conservation Habitat-loss Population structure
- Amos, W., 1999. A comparative approach to the study of microsatellite evolution. In Goldstein, D. B. & C. Schlotterer (eds), Microsatellites: evolution and application. Oxford University Press, USA.Google Scholar
- Askew, R. R., 1988. The dragonflies of Europe. Harley Books, Essex, England.Google Scholar
- Chippindale, P. T., V. K. Davé, D. H. Whitmore & J. V. Robinson, 1999. Phylogenetic relationships of North American damselflies of the genus Ischnura (Odonata: Zygoptera: Coenagrionidae) based on sequences of three mitochondrial genes. Molecular Phylogenetics and Evolution 11: 854–860.CrossRefGoogle Scholar
- Corbet, P. S., 1999. Dragonflies: behaviour and ecology of Odonata, revised edition. Harley Books, Essex, England.Google Scholar
- Dijkstra, K. D. B. & R. Lewington, 2006. Field guide to the dragonflies of Britain and Europe including western Turkey and north-western Africa, British Wildlife Publishing, Milton on Stour.Google Scholar
- Goudet, J., 1995. fstat (vers. 1.2): a computer program to calculate F-statistics. Journal of Heredity 86:485–486.Google Scholar
- Goudet, J., 1999. pca-general for Windows, ver. 1.2. http://www2.unil.ch/izea/softwares/pcagen.html.
- Miller, M. N. & O. M. Fincke, 2004. Mistakes in sexual recognition among sympatric Zygoptera vary with time of day and colour polymorphism (Odonata: Coenagrionidae). International Journal of Odonatology 7: 471–491.Google Scholar
- Pritchard, J. K. & W. Wen, 2003. Documentation for structure software: version 2. http://pritch.bsd.uchicago.edu.
- Smallshire, D. & A. Swash, 2004. Britain’s dragonflies. Wild Guides Ltd, Hampshire, UK.Google Scholar
- Tynkkynen, K., A. Grapputo, J. S. Kotiaho, M. J. Rantala, S. Vaananen & J. Suhonen, 2007. Hybridization in Calopteryx damselflies: the role of males. Animal Behaviour. doi: 10.1016/j.anbehav.2007.09.017.
- Van Tol, J. & M. Verdonk, 1988. Protection des libellules (Odonates) et de leurs biotopes. Conseil de l’Europe, comité européen pour la sauvegarde de la nature et des ressources naturelles, Strasbourg. Collection Sauvegarde de la Nature 38: 188.Google Scholar
- Watts, P. C., D. J. Thompson & S. J. Kemp, 2004a. Cross-species amplification of microsatellite loci in some European zygopteran species (Odonata: Coenagrionidae). International Journal of Odonatology 7: 87–96.Google Scholar
- Wright, S., 1951. The genetical structure of populations. Annals of Eugenics 15: 323–354.Google Scholar