The study of antagonistic interactions among pelagic bacteria: a promising way to coin environmental friendly antifouling compounds
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Ten strains of marine bacteria (SCH0401–SCH0410) were isolated from Ayajin, the east coast of South Korea. In spectrophotometer based chemotaxis assay the ethyl acetate extract (300 μg) of SCH0402 decreased the optical density (OD) of the motile target strains SCH0401, SCH0402, SCH0407 and SCH0408 by two to six times when compared to control. Tributyltin oxide (TBTO) decreased the OD of all target strains by only two times. The most active strain SCH0402 was identified as Shewanella oneidensis by using 16S rDNA gene sequence analysis. Similarly, the target motile strains SCH0401, SCH0402, SCH0407 and SCH0408 were identified as Alteromonas marina, Shewanella oneidensis, Roseobacter gallaeciensis and Bacillus atrophaeus, respectively. The growth inhibition zone produced by the test bacterial extracts against the target strains were three to eight times smaller when compared to that of TBTO. Even though, SCH0402 showed six times weaker antibacterial activity, the repellent activity was three times stronger than TBTO. Therefore, the higher negative chemotactic activity would be better to select eco-friendly antifouling compounds than the other antibacterial activities.
KeywordsAlteromonas marina bacterial repellent biofilm secondary metabolites Shewanella oneidensis 16S rDNA
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- Chun J. 1995. Computer-assisted classification and identification of Actinomycetes. Ph.D. Thesis, University of Newcastle, Newcastle upon Tyne, UKGoogle Scholar
- Felsenstein J. (1993). PHYLIP (Phylogeny Inference Package), version 3.5c. Department of Genetics, University of Washington, Seattle, WA, USAGoogle Scholar
- Giovannoni S. and Rappé M. (2000). Evolution, diversity and molecular ecology of marine prokaryotes. In: Kirchman, D.L. (eds) Microbial ecology of the ocean, pp 47–84. Wiley-Liss, New YorkGoogle Scholar
- Giovannoni S. J. (1991). The polymerase chain reaction. In: Stackebrandt, E. and Goodfellow, M. (eds) Nucleic Acid Techniques in Bacterial Systematic, pp 177–201. John Wiley & Sons, New YorkGoogle Scholar
- Ista L. K., Callow M. E., Finlay J. A., Coleman S. E., Nolasco A. C., Simons R. H., Callow J. A. and Lopez G. P. (2004). Effects of substratum surface chemistry and surface energy on attachment of marine bacteria and algal spores. Applied and Environmental Microbiology 70: 4151–4157PubMedCrossRefGoogle Scholar
- Kwon K. K, Lee H. S., Jung S. Y., Yim J. H., Lee J. H. and Lee H. K. (2002). Isolation and identification of biofilm-forming marine bacteria on glass surfaces in Dae-Ho Dike, Korea. Journal of Microbiology and Biotechnology 40: 260–266Google Scholar
- Lewis J. A. (1998). Marine biofouling and its prevention on underwater surface. Material Forum 22: 41–46Google Scholar
- Pesci E. C., Milbank J. B., Pearson J. P., McKnight S., Kende A. S., Greenberg E. P. and Iglewski B. H. (1999). Quinolone signaling in the cell-to-cell communication system of Pseudomonas aeruginosa. Proceedings of the National Academy of Sciences of the United States of America 96: 11229–11234PubMedCrossRefGoogle Scholar
- Shewan, J. M., 1977. The bacteriology of fresh and spoiling fish and the biochemical changes induced by bacterial action. In Proceedings of the Conference of Handling, Processing and Marketing of Tropical Fish, Tropical Product Institute, London, 51–66Google Scholar
- Venkateswaran K., Moser D. P., Dolhopf M. E., Lies D. P., Saffarini D. A., MacGregor B. J., Ringelberg D. B., White D. C., Nishijima M., Sano H., Burghardt J., Stackebrandt E. and Nealson K. H. (1999). Polyphasic taxonomy of the genus Shewanella and description of Shewanella oneidensis sp. nov. International Journal of Systematic Bacteriology 49: 705–724PubMedCrossRefGoogle Scholar