Hydrobiologia

, Volume 569, Issue 1, pp 129–133 | Cite as

Molecular characterization of proteinaceous material in the Florida coastal Everglades

  • Vera Jones
  • Kathleen Parish
  • Anu Thomson
  • George A. Wolff
  • Nagamitsu Maie
  • Rudolf Jaffé

Abstract

Dissolved organic nitrogen (DON) is the least known component of the nitrogen cycle, in part as a result of the lack of adequate analytical methods for its molecular characterization. In this study proteinaceous material in DON, collected at six geomorphologically different sites in the Florida coastal Everglades, was characterized by amino acid analysis and protein gel electrophoresis. The amino acid composition of the samples suggests that the canal DON was more degraded and subject to higher microbial inputs than the mangrove marshwater and marine end-member stations. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) results supported this observation as distinctly different protein profiles were obtained for the canal waters compared to samples collected at other stations. These preliminary results highlight the potential of combining amino acid and intact protein analysis to fingerprint the sources of DON in different aquatic environments, and show SDS-PAGE as a potentially useful method to characterize DON.

Keywords

amino acids electrophoresis dissolved organic nitrogen microbial proteins 

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Copyright information

© Springer 2006

Authors and Affiliations

  • Vera Jones
    • 1
  • Kathleen Parish
    • 2
  • Anu Thomson
    • 1
  • George A. Wolff
    • 1
  • Nagamitsu Maie
    • 2
  • Rudolf Jaffé
    • 2
  1. 1.Atkins WaterChadwick HouseBirchwood Science Park, Warrington, CheshireUK
  2. 2.Southeast Environmental Research Center and Department of ChemistryFlorida International UniversityMiamiUSA

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