, Volume 550, Issue 1, pp 29–45

Effects of nutrient enrichments on primary production in the Ria Formosa coastal lagoon (Southern Portugal)



Small-scale, short-term enrichment experiments were conducted in the Ria Formosa coastal lagoon (southern Portugal), to assess the effects of nutrient availability on primary productivity, biomass (as chlorophyll a), and algal composition. Samples were collected from natural communities at three different sites in the western lagoon: Barra, oceanic inlet; Ramalhete, adjacent to a urban waste water treatment plant; and Ponte, an upstream channel used for recreation and bivalve farming. These samples were enriched separately with nitrogen and phosphorous during the extreme neap tides of the summer solstice at both high (HW) and low water (LW). The experiment was repeated during the autumnal equinox to test for seasonality, and during the following summer solstice to test for replication. The addition of nitrogen consistently stimulated the productivity and biomass during summer experiments at the two sites within the lagoon, identifying N as the most likely primary “potentially limiting nutrient” in the western part of the lagoon for this period. No stimulation of biomass and productivity occurred in September at the same two sites indicating the importance of other factors such as light, sedimentation or grazing pressure, as controlling the pelagic community. However, these outcomes were reversed at the oceanic inlet (Barra-HW) where there was no stimulation by nitrogen during the summer months, but there was in September, suggesting that there is a different nutritional requirement for the coastal community in comparison with the lagoon community. In samples where productivity was stimulated, diatoms were the group most sensitive to enrichment.


nutrient limitation primary production bioassay diatom stimulation coastal lagoon 


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  1. Affronti, L. F., Marshall, H. G. 1993Diel abundance and productivity patterns of autotrophic picoplankton in the lower Chesapeake BayJournal of Plankton Research1518Google Scholar
  2. Agawin, N. S. R., Duarte, C. M., Agusti, S. 2000Nutrient and temperature control of the contribution of picoplankton to phytoplankton biomass and productionLimnology and Oceanography45591600Google Scholar
  3. Asmus, R. M., Sprung, M., Asmus, H. 2000Nutrient fluxes in intertidal communities of a South European lagoon (Ria Formosa)-similarities and differences with a northern Wadden Sea bay (Sylt-Romo Bay)Hydrobiologia436217235CrossRefGoogle Scholar
  4. Ault, T., Velzeboer, R., Zammit, R. 2000Influence of nutrient availability on phytoplankton growth and community structure in the Port Adelaide River, Australia:bioassay assessment of potential nutrient limitationHydrobiologia42989103CrossRefGoogle Scholar
  5. Austen, M. C., Warwick, R. M., Rosado, M. C. 1989Meiobenthic and macrobenthic community structure along a putative pollution gradient in Southern PortugalMarine Pollution Bulletin20398405CrossRefGoogle Scholar
  6. Berdalet, E., Marrasé, C., Estrada, M., Arin, L., MacLean, M.L. 1996Microbial community responses to nitrogen- and phosphorus-deficient nutrient inputs: microplankton dynamics and biochemical characterizationJournal of Plankton Research1816271641Google Scholar
  7. Bishop, S. S., Emmanuele, K. A., Yoder, J. A. 1984Nutrient limitation of phytoplankton growth in Georgia Nearshore watersEstuaries7506512Google Scholar
  8. Bricker, S. B., Clement, C. G., Pirhalla, D. E., Orlando, S. P., Farrow, D. R. G. 1999National Estuarine Eutrophication Assessment: effects of nutrient enrichment in the Nation’s EstuariesNational Ocean ServiceSilver Spring, MD, USAGoogle Scholar
  9. Bryan, J. R., Riley, J. P., leB Williams, P. J. 1976A Winkler procedure for making precise measures of oxygen concentration for productivity and related studiesJournal of Experimental Marine Biology and Ecology21191197CrossRefGoogle Scholar
  10. Caraco, N., Tamse, A., Boutros, O., Valiela, I. 1987Nutrient limitation of phytoplankton growth in brackish coastal pondsCanadian Journal of Fisheries and Aquatic Sciences44473476Google Scholar
  11. Carlsson, P., Granéli, E. 1999Effects of N:P:Si ratios and zooplankton grazing on phytoplankton communities in the northern Adriatic Sea. II. Phytoplankton species compositionAquatic Microbial Ecology185565Google Scholar
  12. Clarke, K. R., Warwick, R. M. 2001Change in Marine Communities: An Approach to Statistical Analysis and Interpretation2 Primer-EPlymouth, UKGoogle Scholar
  13. Conley, D. J. 2000Biogeochemical nutrient cycles and nutrient management strategiesHydrobiologia4108796CrossRefGoogle Scholar
  14. Diário da República, 2001. Portaria no. 704/2001. DR 159 SÉRIE I-B, 2001-07–11Google Scholar
  15. De Baar, H. J. W., 1994. von Liebig’s Law of the Minimum and Plankton Ecology (1899–1991). Progress in Oceanography 33: 347–386Google Scholar
  16. Dufour, P., Berland, B. 1999Nutrient control of phytoplanktonic biomass in atoll lagoons and Pacific ocean waters: studies with factorial enrichment bioassaysJournal of Experimental Marine Biology and Ecology234147166CrossRefGoogle Scholar
  17. Edwards, V. R., Tett, P., Jones, K. J. 2003Changes in the yield of chlorophyll from dissolved available inorganic nitrogen after an enrichment event – applications for predicting eutrophication in coastal watersContinental Shelf Research2317711786CrossRefGoogle Scholar
  18. Edwards, V., J. Icely, A. Newton & R. Webster, In Press. The yield of chlorophyll from nitrogen: a comparison between the shallow Ria Formosa lagoon and the deep oceanic conditions at Sagres along the southern coast of Portugal. Estuarine, Coastal and Shelf ScienceGoogle Scholar
  19. Elser, J. J., Kimmel, B. L. 1986Alteration of phytoplankton phosphorus status during enrichment experiments: implications for interpreting nutrient enrichment bioassay resultsHydrobiologia133217222Google Scholar
  20. Falcão, M., Vale, C. 1990Study of the Ria Formosa ecosystem: benthic nutrient remineralization and tidal variability of nutrients in the waterHydrobiologia207137146CrossRefGoogle Scholar
  21. Falcão, M., Vale, C. 1998Sediment-water exchanges of ammonium and phosphate in intertidal and subtidal areas of a mesotidal coastal lagoon (Ria Formosa)Hydrobiologia373/374193201CrossRefGoogle Scholar
  22. Falcão, M., Vale, C. 2003Nutrient dynamics in a coastal lagoon (Ria Formosa, Portugal): the importance of lagoon-sea water exchanges on the biological productivityCiencias Marinas29425433Google Scholar
  23. Ferreira, J. G., Simas, T., Nobre, A., Silva, M.C., Shifferegger, K., Lencart-Silva, J. 2003Identification of sensitive areas and vulnerable zones in transitional and coastal Portuguese systemsINAG & IMARPortugalGoogle Scholar
  24. Fiúza, A. F. G., Macedo, M. E., Guerreiro, M. R. 1982Climatological space and time variation of the Portuguese coastal upwellingOceanologica Acta53140Google Scholar
  25. Fong, P., Zedler, J. B., Donohoe, R. M. 1993Nitrogen vs. phosphorus limitation of algal biomass in shallow coastal lagoonsLimnology and Oceanography38906923Google Scholar
  26. Gamito, S. 1997aSustainable management of a coastal lagoon system (Ria Formosa, Portugal): An ecological model for extensive aquacultureInternational Journal of Salt Lake Research6145173CrossRefGoogle Scholar
  27. Gamito, S. 1997bApplication of canonical correspondence analysis to environmental and benthic macrofauna data of four sites in the Ria Formosa (Portugal)Publicaciones Especiales Instituto Español de Oceanografia234152Google Scholar
  28. Gowen, R. J., Tett, P., Jones, K. J. 1992Predicting marine eutrophication: the yield of chlorophyll from nitrogen in Scottish coastal watersMarine Ecology Progress Series85153161Google Scholar
  29. Granéli, E., Sundbäck, K. 1985The response of planktonic and microbenthic algal assemblages to nutrient enrichment in shallow coastal waters, Southwest SwedenJournal of Experimental Marine Biology and Ecology85253268CrossRefGoogle Scholar
  30. Granéli, E., Granéli, W., Rydberg, L. 1986Nutrient limitation at the ecosystem and the phytoplankton community level in the Laholm Bay, South East KattegatOphelia26181194Google Scholar
  31. Granéli, E. 1987Nutrient limitation of phytoplankton Biomass in a brackish water bay highly influenced by river dischargedEstuarine Coastal and Shelf Science25555565CrossRefGoogle Scholar
  32. Granéli, E., Wallström, K., Larsson, U., Granéli, W., Elmgren, R. 1990Nutrient limitation of Primary Production in the Baltic Sea AreaAmbio19142151Google Scholar
  33. Grasshoff, K., Ehrhardt Kremling, M. K. 1983Methods of Seawater AnalysisVerlag Chemie. FRGermanyGoogle Scholar
  34. Healey, F. P. 1979Short-term responses of nutrient-deficient algae to nutrient additionJournal of Phycology15289299CrossRefGoogle Scholar
  35. Hecky, R. E., Kilham, P. 1988Nutrient limitation of phytoplankton in freshwater and marine environments: a review of recent evidence on the effects of enrichmentLimnology and Oceanography33796822Google Scholar
  36. Howarth, R. W. 1988Nutrient limitation of net primary production in marine ecosystemsAnnual Review of Ecology1989110CrossRefGoogle Scholar
  37. Jacquet, S., Havskum, H., Thingstad, T. F., Vaulot, D. 2002Effects of inorganic and organic nutrient addition on a coastal microbial community (Isejord, Denmark)Marine Ecology Progress Series228314Google Scholar
  38. JGOFS, 1994. Protocols for the joint global ocean flux study (JGOFS) core measurements. Intergovernmental Oceanographic Commission. Manuals and Guides 29Google Scholar
  39. Kudela, R. M., Dugdale, R. C. 2000Nutrient regulation of phytoplankton productivity in Monterey Bay, CaliforniaDeep-Sea Research4710231053CrossRefGoogle Scholar
  40. Liebig, J. 1840Organic Chemistry in its Application to Agriculture and PhysiologyTaylor & Walton LondonGoogle Scholar
  41. Lyngby, J. E. & S. M. Mortensen, 1995. Biomonitoring of eutrophication levels in shallow coastal ecosystems. In Elettheriou, A. (eds), Biology and Ecology of shallow coastal water. Proceedings 28th European Marine Biology Symposium, Iraklio, Crete, 1993. Olsen & Olsen, Fredensborg, International Symposium Series, 39–44Google Scholar
  42. Malone, T. C., Conley, D. J., Fisher, T. R., Glibert, P. M., Harding, L. W., Sellner, K. G. 1996Scales of nutrient-limited phytoplankton productivity in Chesapeake BayEstuaries19371385Google Scholar
  43. Maranon, E., Fernandez, E., Anadon, R. 1995Patterns of macromolecular synthesis by natural phytoplankton assemblages under changing upwelling regimes: in situ observations and microcosm experimentsJournal of Experimental Marine Biology and Ecology188128CrossRefGoogle Scholar
  44. Marques, A., Coimbra, R., Sprung, M., Andrade, J. P. 1996Plankton and its environment: studies in shallow tidal creeks in a Ria Formosa salt marshCiência Biológica Ecology and Systematics (Portugal)163543Google Scholar
  45. Marshall, H. G. 1995Autotrophic picoplankton distribution and abundance in the Chesapeake Bay, U.S.AMarine Nature43342Google Scholar
  46. Newton, A. & J. D. Icely, 2002. Impact of Coastal Engineering on the Water Quality of the Ria Formosa Lagoon, Portugal. In Eurocoast (eds), Littoral 2002, The Changing Coast. Ed. Eurocoast, Porto, Portugal, 417–421Google Scholar
  47. Newton, A., Icely, J., Falcão, M., Nobre, A., Nunes, J. P., Ferreira, J. G., Vale, C. 2003Evaluation of Eutrophication in the Ria Formosa Coastal Lagoon, PortugalContinental Shelf Research2319451961CrossRefGoogle Scholar
  48. Newton, A., Mudge, S. 2003Temperature and salinity regimes in a shallow, mesotidal lagoon, the Ria Formosa, PortugalEstuarine Coastal and Shelf Science56113CrossRefGoogle Scholar
  49. Nixon, S. W. 1981

    Remineralization and nutrient cycling in coastal marine ecosystems

    Neilson, B. J.Cronin, L. E. eds. Estuaries and nutrientsThe Humana PressNew Jersey, USA111137
    Google Scholar
  50. Nixon, S. W., 1982. Nutrient dynamics, primary production and fisheries yields of lagoons. Proceedings of the International Symposium Coastal Lagoons, SCOR/IABO/UNESCO, Bordeaux, France, 8–14 Sep., 1981. Oceanologica Actal. 357–371Google Scholar
  51. Officer, C. B., Ryther, J. H. 1980The possible importance of Silicon in marine eutrophicationMarine Ecology Progress Series38391Google Scholar
  52. Oviatt, C., Doering, P., Nowicki, B., Reed, L., Cole, J., Frithsen, J. 1995An ecosystem level experiment on nutrient limitation in temperate coastal marine environmentsMarine Ecology Progress Series116171179Google Scholar
  53. Pennock, J. R., Sharp, J. H. 1994Temporal alteration between light- and nutrient-limitation of phytoplankton in a coastal plain estuaryMarine Ecology Progress Series142261272Google Scholar
  54. Pitcher, G. C., Bolton, J. J., Brown, P. C., Hutchings, L. 1993The development of phytoplankton blooms in upwelled waters of the southern Benguela upwelling system as determined by microcosm experimentsJournal of Experimental Marine Biology and Ecology165171189CrossRefGoogle Scholar
  55. Reis, C. & M. Sprung, 1995. Biomassa e distribuição macroalgal na Ria Formosa. 8° Congresso do Algarve: 911–917Google Scholar
  56. Robertson A. I., 1999. Limiting nutrient workshop 1997. LWRRDC, Occasional Paper 7/99Google Scholar
  57. Sanders, J. G., Cibik, S. T., D’Elia, C. F., Boynton, W. R. 1987Nutrient enrichment studies in a coastal plain estuary: changes in phytoplankton species compositionCanadian Journal of Fisheries and Aquatic Sciences448390Google Scholar
  58. Schindler, D. W. 1974Eutrophication and recovery in experimental lakes: implications for lake managementScience184897899Google Scholar
  59. Schülter, L. 1998The influence of nutrient addition on growth rates of phytoplankton groups, and microzooplankton grazing rates in a mesocosm experimentJournal of Experimental Marine Biology and Ecology2285371CrossRefGoogle Scholar
  60. Smith, S. V. 1984Phosphorus versus nitrogen limitation in the marine environmentLimnology and Oceanography2911491160Google Scholar
  61. Sprung, M. 1994Macrobenthic secondary production in the intertidal zone of the Ria Formosa – a Lagoon in Southern PortugalEstuarine Coastal and Shelf Science38539558CrossRefGoogle Scholar
  62. Strickland, J. D. H., T. R. Parsons, 1972. A pratical handbook of seawater analysis. 2nd edn. Ottawa: Fisheries Research Board of Canada Bulletin 167, 311 ppGoogle Scholar
  63. Taylor, D. I., Nixon, S. W., Granger, S. L., Buckley, B. A. 1999Responses of Coastal Lagoon plant communities to levels of nutrient enrichment: a Mesocosm StudyEstuaries2210411056Google Scholar
  64. Tett, P., Gilpin, L., Svendsen, H., Erlandsson, C. P., Larsson, U., Kratzer, S., Fouilland, E., Janzen, C., Lee, J. Y., Grenz, C., Newton, A., Ferreira, J. G., Fernandes, T., Scory, S. 2003Eutrophication and some European waters of restricted exchangeContinental Shelf Research2316351671CrossRefGoogle Scholar
  65. Tomas, C. R. 1997Identifying Marine PhytoplanktonAcademic PressLondonGoogle Scholar
  66. Tyrrell, T., Law, C. S. 1997Low nitrate:phosphate ratios in the global oceanNature387793796CrossRefGoogle Scholar
  67. Tyrrell, T. 1999The relative influences of nitrogen and phosphorus on oceanic primary productionNature400525531CrossRefGoogle Scholar
  68. Utermöhl, H. 1958Zur Vervolkommnung der quantitativen Phytoplankton-Methodik. Mitteilungen der Internationalen Vereinigung fur Theoretische und AngewandteLimnologie9138Google Scholar
  69. Vale, P., Sampayo, M. A. M. 1999Esters of okadaic acid and dinophysistoxin-2 in Portuguese bivalves related to human poisoningsToxicon3711091121CrossRefPubMedGoogle Scholar
  70. Vallejo, S. M. A., 1982. Development and management of coastal lagoons. Proceedings of the International Symposium Coastal Lagoons, SCOR/IABO/UNESCO, Bordeaux, France, 8–14 September, 1981. Oceanologica Acta: 397–401Google Scholar

Copyright information

© Springer 2005

Authors and Affiliations

  1. 1.CMQA/FCTUniversidade do Algarve FaroPortugal
  2. 2.IMAR/FCTUniversidade do AlgarveFaroPortugal
  3. 3.Sagremarisco LdaVila do BispoPortugal

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