Journal of Molecular Histology

, Volume 50, Issue 3, pp 179–188 | Cite as

Expression of CPNE7 during mouse dentinogenesis

  • Yeoung-Hyun Park
  • Yoon Seon Lee
  • Jung-Su Park
  • Seung Hee Kim
  • Hyun Sook Bae
  • Joo-Cheol ParkEmail author
Original Paper


Interactions between the ectodermal and mesenchymal tissues are the basis of the central mechanism regulating tooth development. Based on this epithelial-mesenchymal interaction (EMI), we demonstrated that copine-7 (CPNE7) is secreted by preameloblasts and regulates the differentiation of mesenchymal cells of dental or non-dental origin into odontoblasts. However, the precise expression patterns of CPNE7 in the stages of tooth development have not yet been elucidated. The aim of the present study was to establish the spatiotemporal expression pattern of CPNE7 during mouse tooth development. To examine the spatiotemporal expression patterns of CPNE7 during mouse tooth development, we investigate the distribution of CPNE7 in the embryonic and postnatal developing mouse tooth. Immunohistochemistry, in situ hybridization, real-time PCR, and western blot analysis are performed to investigate the CPNE7 expression pattern during tooth development of the mandibular mouse first molar. During the initiation stage (bud stage), CPNE7 protein expression is observed in the dental epithelium but not yet in the dental mesenchyme. At E18 (bell stage), expression of CPNE7 protein and mRNA is primarily observed in ectomesenchymal cells of dental papilla. At P7 (crown formation stage), CPNE7 is localized in differentiating odontoblasts but weak expression is detected in mature ameloblasts. These findings suggest that CPNE7 secreted by dental epithelium induces the differentiation of ectomesenchymal cells into preodontoblast in concert with EMI. CPNE7 is clearly expressed in differentiating odontoblasts and the odontoblast process during dentinogenesis, but is no longer expressed in fully differentiated odontoblasts. Furthermore, CPNE7 is expressed in the Hertwig’s epithelial root sheath (HERS) and in the facing preodontoblasts during root dentin formation. Taken together, these results illustrate the dynamic expression of CPNE7 during tooth development and suggest its important function in entire stages of tooth development.


CPNE7 Epithelial-mesenchymal interaction Odontoblast Odontoblast process 



This work was supported by Basic Science Research Program through the Ministry of Education of the Republic of Korea and National Research Foundation (Grant Nos. NRF-2016R1A2B3006584 and NRF-2018R1A5A2024418).

Compliance with ethical standards

Conflict of interest

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be constructed as a potential conflict of interest.


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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.Laboratory for the Study of Regenerative Dental Medicine, Department of Oral Histology-Developmental Biology, School of Dentistry and Dental Research InstituteSeoul National UniversitySeoulRepublic of Korea
  2. 2.Department of Dental HygieneSeoyeong UniversityGwang-juRepublic of Korea
  3. 3.Department of Dental HygieneGwangju Health UniversityGwang-juRepublic of Korea
  4. 4.Department of Oral HygieneNamseoul UniversityCheonanRepublic of Korea

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